Sequence analysis of the hypervariable region in &lt;i&gt;hmtp210&lt;/i&gt; of &lt;i&gt;Avibacterium paragallinarum&lt;/i&gt;

Araya-Hidalgo, Edward; Gutiérrez-Jiménez, Cristina; Chaves-Ramírez, Mónica; Suárez-Esquivel, Marcela; Guzmán-Verri, Caterina; Barquero-Calvo, Elías

doi:10.1292/jvms.16-0530

Cited by 7 publications

(2 citation statements)

References 18 publications

(29 reference statements)

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“…Sequences were not clustered according to Page's serovar scheme of classification. This result was in agreement with findings reported by other researchers (2,3,23).…”

Section: Detection Of a Paragallinarum And Viral Pathogens By Pcr Rsupporting

confidence: 94%

First report on the molecular detection, phylogeny, virological and pathological investigations of Avibacterium paragallinarum in chickens in Turkey

Umar¹,

Öngör²,

Bayraktar³

et al. 2020

Medycyna Weterynaryjna

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Avibacterium paragallinarum is an important pathogen affecting the respiratory tract of chickens. There is a paucity of information on the molecular characteristics and pathology of A. paragallinarum in Turkish poultry flocks. In the present study, broiler and layer flocks (n = 2) suspected of viral infections with serious respiratory signs and significant mortality were visited. Chickens showed various disease signs and necropsy lesions, including purulent nasal discharge, respiratory distress, facial edema, sticky eyes, mucoid tracheitis, hemorrhagic inflammation of the infraorbital sinuses along with fibrinous mass and conjunctivitis. Histopathological lesions included loss of cilia along with necrosis and exfoliation of the superficial mucosal epithelium of the trachea, facial cellulitis, dermatitis, fibrinous plasmatic edema and infiltration of inflammatory cells, especially heterophils. A. paragallinarum was detected in tissue samples by species-specific polymerase chain reaction (PCR). The sequencing and phylogenetic analysis of the core region of the hemagglutinin (HA) gene revealed that Turkish strains detected here belonged to serotype A (serovar A1). They were related to strains reported from India (VRDC), the United States (0083), and Japan (0221), which are representatives of serovar A1. A homology of 88-90% was found between Indian strains and the Turkish strains detected in this study. Surprisingly, only vaccine strains of infectious bronchitis virus (IBV) were detected as a co-infecting agent in all samples positive for A. paragallinarum. Our findings suggest that A. paragallinarum may be an emerging pathogen in Turkish poultry flocks, and direct PCR may facilitate rapid diagnosis of infectious coryza. These results will also help to develop control strategies for A. paragallinarum.

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“…Sequences were not clustered according to Page's serovar scheme of classification. This result was in agreement with findings reported by other researchers (2,3,23).…”

Section: Detection Of a Paragallinarum And Viral Pathogens By Pcr Rsupporting

confidence: 94%

First report on the molecular detection, phylogeny, virological and pathological investigations of Avibacterium paragallinarum in chickens in Turkey

Umar¹,

Öngör²,

Bayraktar³

et al. 2020

Medycyna Weterynaryjna

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show abstract

“…Furthermore, the TAA HMTp210 is the major hemagglutinin antigen of Avibacterium paragallinarum, which can cause infectious coryza in poultry. A variable region within HMTp210 is proposed as a candidate for recombinant vaccine production [247][248][249]. Lastly, Actinobacillus pleuropneumoniae adhesin 1 (Apa1) and 2 (Apa2) are two TAAs expressed on the bacterial surface of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia.…”

Section: Other Taasmentioning

confidence: 99%

Immunogenicity of trimeric autotransporter adhesins and their potential as vaccine targets

Thibau

Dichter

Vaca

et al. 2019

Med Microbiol Immunol

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The current problem of increasing antibiotic resistance and the resurgence of numerous infections indicate the need for novel vaccination strategies more than ever. In vaccine development, the search for and the selection of adequate vaccine antigens is the first important step. In recent years, bacterial outer membrane proteins have become of major interest, as they are the main proteins interacting with the extracellular environment. Trimeric autotransporter adhesins (TAAs) are important virulence factors in many Gram-negative bacteria, are localised on the bacterial surface, and mediate the first adherence to host cells in the course of infection. One example is the Neisseria adhesin A (NadA), which is currently used as a subunit in a licensed vaccine against Neisseria meningitidis. Other TAAs that seem promising vaccine candidates are the Acinetobacter trimeric autotransporter (Ata), the Haemophilus influenzae adhesin (Hia), and TAAs of the genus Bartonella. Here, we review the suitability of various TAAs as vaccine candidates.

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Identification and characterization of DutchAvibacterium paragallinarumisolates and the implications for diagnostics

et al. 2019

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Sequence analysis of the hypervariable region in <i>hmtp210</i> of <i>Avibacterium paragallinarum</i>

Cited by 7 publications

References 18 publications

First report on the molecular detection, phylogeny, virological and pathological investigations of Avibacterium paragallinarum in chickens in Turkey

First report on the molecular detection, phylogeny, virological and pathological investigations of Avibacterium paragallinarum in chickens in Turkey

Immunogenicity of trimeric autotransporter adhesins and their potential as vaccine targets

Identification and characterization of DutchAvibacterium paragallinarumisolates and the implications for diagnostics

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