Solar UV-B radiation modulates chemical defenses against Anticarsia gemmatalis larvae in leaves of field-grown soybean

Dillon, Francisco M.; Chludil, Hugo D.; Zavala, Jorge A.

doi:10.1016/j.phytochem.2017.05.006

Cited by 18 publications

(19 citation statements)

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“…Chlorogenic acid was also related to susceptibility but has been related to insect resistance in other plant species ( e.g. Leiss et al 2009 ; Dillon et al 2017 ). In our study, chlorogenic acid was particularly abundant in one of our most susceptible C. annuum accessions in the flowering stage (RU 43).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses

et al. 2019

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The development of pesticide resistance in insects and recent bans on pesticides call for the identification of natural sources of resistance in crops. Here, we used natural variation in pepper ( Capsicum spp.) resistance combined with an untargeted metabolomics approach to detect secondary metabolites related to thrips ( Frankliniella occidentalis ) resistance. Using leaf disc choice assays, we tested 11 Capsicum accessions of C. annuum and C. chinense in both vegetative and flowering stages for thrips resistance. Metabolites in the leaves of these 11 accessions were analyzed using LC-MS based untargeted metabolomics. The choice assays showed significant differences among the accessions in thrips feeding damage. The level of resistance depended on plant developmental stage. Metabolomics analyses showed differences in metabolomes among the Capsicum species and plant developmental stages. Moreover, metabolomic profiles of resistant and susceptible accessions differed. Monomer and dimer acyclic diterpene glycosides (capsianosides) were pinpointed as metabolites that were related to thrips resistance. Sucrose and malonylated flavone glycosides were related to susceptibility. To our knowledge, this is the first time that dimer capsianosides of pepper have been linked to insect resistance. Our results show the potential of untargeted metabolomics as a tool for discovering metabolites that are important in plant – insect interactions. Electronic supplementary material The online version of this article (10.1007/s10886-019-01074-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses

et al. 2019

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“…The wavelength of the UV detector was set at λ 254, 270, and 360 nm. Quantification of flavonoids was done as rutin or genistin equivalents, as described in Dillon et al (). Analytical and HPLC degree solvents used for determination of leaf phenolic compounds were purchased from Sintorgan (Argentina).…”

Section: Methodsmentioning

confidence: 99%

“…Plant constitutive and inducible defenses are modulated by variations in light quality generated by the canopy and associated to plant density (Ballaré, Mazza, Austin, & Pierik, ). Solar ultraviolet‐B (UV‐B; λ 280–315 nm) radiation is not only used by plants to sense competition (Boccalandro, Mazza, Mazzella, Casal, & Ballaré, ) but also increases plant resistance to herbivorous insects under field conditions (Ballaré, Cadlwell, Flint, Robinson, & Bornman, ), including soybean crops (Dillon, Chludil, & Zavala, ; Mazza, Giménez, Kantolic, & Ballaré, ; Zavala, Mazza, Dillon, Chludil, & Ballaré, ; Zavala, Scopel, & Ballaré, ). One of the main defenses induced typically by solar UV‐B radiation in leaves are the phenolic compounds (Ballaré et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Solar UV‐B radiation induced transcription of chalcone synthase , flavanone 3 β‐hydroxylase , and flavonol synthase genes in soybean, increasing flavonol content in leaves (Jenkins, ; Kim, Kim, Kim, Lee, & Ahn, ). Moreover, other type of flavonoids, such as isoflavonoids can be induced in damaged soybean leaves by lepidopteran larvae, which negatively affected larval growth (Dillon et al, ; Murakami et al, ; O'Neill et al, ; Piubelli, Hoffmann‐Campo, Moscardi, Miyakubo, & Neves De Oliveira, ). Isoflavonoid production in soybean is mainly regulated by isoflavone synthase (IFS) enzymes, and although expression of the IFS2 gene is induced by biotic and abiotic stresses, IFS1 expression is not affected by these environmental stresses (Dhaubhadel, McGarvey, Williams, & Gijzen, ; Gutierrez‐Gonzalez, Sleper, & Nguyen, ).…”

Section: Introductionmentioning

confidence: 99%

“…Two types of TPI have been characterized in soybean (Kunitz, ; Birk, Gertler, & Khalef, ). TPI are induced in soybean leaves after A. gemmatalis attack and other chewing insects, such as Spodoptera litura and Popillia japonica , which induced the expression of Kunitz type and Bowman‐Birk type inhibitors (Casteel et al, ; da Silva Fortunato et al, ; Dillon et al, ; Wang, Wang, Fan, Yang, & Yu, ). TPI induction in response to damage or to methyl jasmonate (MeJA) applications is enhanced by solar UV‐B radiation in Nicotiana sp.…”

Section: Introductionmentioning

confidence: 99%

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SolarUV‐Bradiation and ethylene play a key role in modulating effective defenses againstAnticarsia gemmatalislarvae in field‐grown soybean

Dillon

Tejedor

Ilina

et al. 2017

Plant Cell & Environment

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Solar UV-B radiation has been reported to enhance plant defenses against herbivore insects in many species. However, the mechanism and traits involved in the UV-B mediated increment of plant resistance are unknown in crops species, such as soybean. Here, we studied defense-related responses in undamaged and Anticarsia gemmatalis larvae-damaged leaves of two soybean cultivars grown under attenuated or full solar UV-B radiation. We determined changes in jasmonates, ethylene (ET), salicylic acid, trypsin protease inhibitor activity, flavonoids, and mRNA expression of genes related with defenses. ET emission induced by Anticarsia gemmatalis damage was synergistically increased in plants grown under solar UV-B radiation and was positively correlated with malonyl genistin concentration, trypsin proteinase inhibitor activity and expression of IFS2, and the pathogenesis protein PR2, while was negatively correlated with leaf consumption. The precursor of ET, aminocyclopropane-carboxylic acid, applied exogenously to soybean was sufficient to strongly induce leaf isoflavonoids. Our results showed that in field-grown soybean isoflavonoids were regulated by both herbivory and solar UV-B inducible ET, whereas flavonols were regulated by solar UV-B radiation only and not by herbivory or ET. Our study suggests that, although ET can modulate UV-B-mediated priming of inducible plant defenses, some plant defenses, such as isoflavonoids, are regulated by ET alone.

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Leaf me alone:Medicago truncatuladefenses against foliar lepidopteran herbivores

Bede

2019

The Model Legume Medicago Truncatula

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Solar UV-B radiation modulates chemical defenses against Anticarsia gemmatalis larvae in leaves of field-grown soybean

Cited by 18 publications

References 58 publications

Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses

Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses

SolarUV‐Bradiation and ethylene play a key role in modulating effective defenses againstAnticarsia gemmatalislarvae in field‐grown soybean

Leaf me alone:Medicago truncatuladefenses against foliar lepidopteran herbivores

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