Structural and biochemical insights into the 2′‐<i>O</i>‐methylation of pyrimidines 34 in <scp>tRNA</scp>

Pang, Panjiao; Deng, Xiaogeng; Wang, Zhong; Xie, Wei

doi:10.1111/febs.14120

Cited by 6 publications

(3 citation statements)

References 39 publications

(74 reference statements)

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“…As a consequence of its pervasive presence, MTA regulates, directly or indirectly, expression, activity or both, of enzymes involved in methionine, AdoMet or polyamine biosynthesis (Anashkin et al ., ). Being similar to AdoMet, but smaller, MTA often interferes with AdoMet synthesis or other processes (see for example Pang et al ., ), with a variety of consequences on methionine availability, including inhibition of virulence of pathogens and production of antibiotics (Tojo et al ., ; Wang et al ., ; Bourgeois et al ., ). The outcome of this constraint is that the level of the molecule is generally kept low in cells.…”

Section: General Features Of the Standard Aerobic Mspmentioning

confidence: 99%

Revisiting the methionine salvage pathway and its paralogues

Sekowska

Ashida

Danchin

2018

Microbial Biotechnology

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SummaryMethionine is essential for life. Its chemistry makes it fragile in the presence of oxygen. Aerobic living organisms have selected a salvage pathway (the MSP) that uses dioxygen to regenerate methionine, associated to a ratchet‐like step that prevents methionine back degradation. Here, we describe the variation on this theme, developed across the tree of life. Oxygen appeared long after life had developed on Earth. The canonical MSP evolved from ancestors that used both predecessors of ribulose bisphosphate carboxylase oxygenase (RuBisCO) and methanethiol in intermediate steps. We document how these likely promiscuous pathways were also used to metabolize the omnipresent by‐products of S‐adenosylmethionine radical enzymes as well as the aromatic and isoprene skeleton of quinone electron acceptors.

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Section: General Features Of the Standard Aerobic Mspmentioning

confidence: 99%

Revisiting the methionine salvage pathway and its paralogues

Sekowska

Ashida

Danchin

2018

Microbial Biotechnology

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“…This is indeed the case for the bacterial TrmD, a member of the SPOUT trefoil knotted fold family (for SpoU-TrmD), and for the eukaryotic and archaeal class I MTases Trm5, which all catalyze formation of m 1 G 37 (Goto-Ito et al, 2017). The same is true for the bacterial TrmL SPOUT MTase and for the eukaryotic FTSJ1/ WDR6 complex, which both catalyze the S-adenosyl-L-methionine (SAM)-dependent formation of Nm at position 34 in tRNA Leu (Guy et al, 2012;Pang et al, 2017). Even more striking is the case of the m 5 U 54 (or ribothymidine) tRNA modification.…”

Section: Introductionmentioning

confidence: 94%

Division of labor in epitranscriptomics: What have we learnt from the structures of eukaryotic and viral multimeric RNA methyltransferases?

Graille

2021

WIREs RNA

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The translation of an mRNA template into the corresponding protein is a highly complex and regulated choreography performed by ribosomes, tRNAs and translation factors. Most RNAs involved in this process are decorated by multiple chemical modifications (known as epitranscriptomic marks) contributing to the efficiency, the fidelity and the regulation of the mRNA translation process. Many of these epitranscriptomic marks are written by holoenzymes made of a catalytic subunit associated with an activating subunit. These holoenzymes play critical roles in cell development.Indeed, several mutations being identified in the genes encoding for those proteins are linked to human pathologies such as cancers and intellectual disorders for instance. This review describes the structural and functional properties of RNA methyl-

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“…The tRNA methyltransferase family is involved in these reactions. Thus, TrmL is the writer that catalyzes the transfer of a methyl group to the 2′-hydroxyl group of the pyrimidines at the wobble position 34 in two tRNA isoacceptors [ 115 , 116 , 117 ]. However, MS analysis has revealed that METTL2 is the enzyme catalyzing 3-methylcytosine (m 3 C) methylation in total tRNA and that METTL6 is the enzyme catalyzing m 3 C in seryl-tRNA [ 118 ].…”

Section: Rna Modificationsmentioning

confidence: 99%

Above the Epitranscriptome: RNA Modifications and Stem Cell Identity

Morena

Argentati

Bazzucchi

et al. 2018

Genes

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Sequence databases and transcriptome-wide mapping have revealed different reversible and dynamic chemical modifications of the nitrogen bases of RNA molecules. Modifications occur in coding RNAs and noncoding-RNAs post-transcriptionally and they can influence the RNA structure, metabolism, and function. The result is the expansion of the variety of the transcriptome. In fact, depending on the type of modification, RNA molecules enter into a specific program exerting the role of the player or/and the target in biological and pathological processes. Many research groups are exploring the role of RNA modifications (alias epitranscriptome) in cell proliferation, survival, and in more specialized activities. More recently, the role of RNA modifications has been also explored in stem cell biology. Our understanding in this context is still in its infancy. Available evidence addresses the role of RNA modifications in self-renewal, commitment, and differentiation processes of stem cells. In this review, we will focus on five epitranscriptomic marks: N6-methyladenosine, N1-methyladenosine, 5-methylcytosine, Pseudouridine (Ψ) and Adenosine-to-Inosine editing. We will provide insights into the function and the distribution of these chemical modifications in coding RNAs and noncoding-RNAs. Mainly, we will emphasize the role of epitranscriptomic mechanisms in the biology of naïve, primed, embryonic, adult, and cancer stem cells.

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Structural and biochemical insights into the 2′‐O‐methylation of pyrimidines 34 in tRNA

Abstract: The atomic coordinates and structural factors have been deposited in the Protein Data Bank with accession number 5CO4.

Cited by 6 publications

References 39 publications

Revisiting the methionine salvage pathway and its paralogues

Revisiting the methionine salvage pathway and its paralogues

Division of labor in epitranscriptomics: What have we learnt from the structures of eukaryotic and viral multimeric RNA methyltransferases?

Above the Epitranscriptome: RNA Modifications and Stem Cell Identity

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