Esophageal cancer (EC) was a kind of disease with vague symptoms, such as difficult to swallow some solid foods, pain with swallowing, or unexplained weight loss. 1 It ranked 7th in incidence and 6th in mortality worldwide, 2 while in China, the incidence rate and mortality of EC were 17.9/100,000 and 13.7/100,000, respectively, 3 and its five-year survival rate was 19%, 4 all of which caused great burden to patients, families, and society. Therefore, it is of great importance to early detection and find new biomarkers to diagnose EC for timely prevention and treatment. In clinical, it was divided into two main histological subtypes: esophageal adenocarcinoma (EAC) and esophageal squamous cell carcinoma (ESCC), 5 while it is estimated that the people in central and southeastern Asia mostly occurred