Zuotai and HgS differ from HgCl2 and methyl mercury in Hg accumulation and toxicity in weanling and aged rats

Zhang, Bin-Bin; Li, Wenkai; Hou, Wei-Yu; Luo, Yan; Shi, Jiannong; Li, Cen; Wei, Lixin; Liu, Jie

doi:10.1016/j.taap.2017.05.021

Cited by 28 publications

(19 citation statements)

References 35 publications

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“…Brie y, 70W at 1 to 5-fold clinical doses and Zuotai (30 mg/kg, po) for 7 days did not produce signi cant effects on the liver CYP450 gene and protein expressions; while HgCl 2 at equivalent Hg dose increased the expression of CYP1A, CYP2B, CYP2E1, and CYP7A at the transcription and/or protein levels, MeHg at 1/10 Hg dose produced similar effects, but to a lesser extent. These results further demonstrated that chemical forms of metals are a major determinant of their biological effects [24][25][26][27][29][30][31].…”

Section: Discussionmentioning

confidence: 63%

“…Mice were given oral administration for consecutive 7 days. The dose regimen selection was based on our prior publications [8,11,[24][25][26][27]. Livers were collected 24 h after the last dose and stored at -80°C prior to analysis.…”

Section: Animal Treatmentsmentioning

confidence: 99%

“…Zuotai is included in 70W in a small amount [2][3][4]8]. The chemical speciation, spatial distribution of mercury from Zuotai are different from that of HgCl 2 [7,18], resulting in differential toxicity when compared to environmental mercury compounds (HgCl 2 and MeHg) in experimental animals and cultured human cells [24][25][26][27][29][30][31]. In patients taking Zuotai-containing Tibetan medicines, mercury toxicity to the liver and kidney was mild, tolerable and reversible [10,[21][22][23].…”

Section: Zuotai In Tibetan Medicinesmentioning

confidence: 99%

“…A recent human study revealed that Zuotai-containing Tibetan medicines are safe at clinical doses [19,21,22], including 70W [23]. Indeed, Zuotai differs from HgCl 2 and MeHg in producing hepatotoxicity [24], nephrotoxicity [25,26], and intestinal toxicity with gut microbiome disruptions [27]. Whether Zuotaicontaining 70W has any effect on CYP450 in comparison with mercury compounds is worth of investigation.…”

Section: Introductionmentioning

confidence: 99%

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Zuotai (β-HgS)-containing 70 Wei Zhen-Zhu-Wan (Rannasangpei) differs from mercury chloride and methylmercury on hepatic cytochrome P450

Nie¹,

Xu²,

Lu³

et al. 2020

Preprint

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Background: Zuotai (mainly β-HgS)-containing 70 Wei-Zhen-Zhu-Wan (70W, Rannasangpei ) is a famous Tibetan medicine for cardiovascular and gastrointestinal diseases. We have shown that 70W protected against CCl 4 hepatotoxicity. CCl 4 is metabolized via cytochrome P450 (CYP450) to produce reactive metabolites. Whether 70W has any effect on CYP450 is unknown and such effects should be compared with mercury compounds for safety evaluation. Methods: Mice were given 70W (0.15-1.5 g/kg, po), Zuotai (30 mg/kg, po), HgCl 2 (33.6 mg/kg, po) and MeHg (3.1 mg/kg, po) for 7 days. Liver RNA and protein were isolated for qPCR and Western-blot analysis. Results: 70W and Zuotai had no effects on hepatic Aryl hydrocarbon receptor (AhR) and CYP1A2, but HgCl 2 and MeHg increased Cyp1a2 mRNA and CYP1A2 protein levels;70W and Zuotai had no effects on constitutive androstane receptor (CAR) ,CYP2B andCYP2E1 expressions, but HgCl 2 increased CAR and Cyp2b10 mRNA, HgCl 2 and MeHg increased CYP2B and CYP2E1 protein expressions; 70W and mercury compounds had no apparent effects on the expression of pregnane X receptor (PXR) and Cyp3a11 mRNA, as well as CYP3A proteins. 70W and mercury compounds had no apparent effects on the expression of peroxisome proliferator-activated receptor alpha (PPARα) and CYP4A; but HgCl 2 tended to increase Cyp4a10 mRNA and CYP4A protein expressions. 70W and Zuotai had no apparent effects on the expression of farnesoid X receptor (FXR) and Cyp7a1 , while HgCl 2 and MeHg increased CYP7A1 expression. Conclusions: Zuotai and Zuotai-containing 70W at clinical doses had minimal influence on hepatic CYPs, and the effects of 70W and Zuotai on CYP and corresponding nuclear receptors are different from HgCl 2 and MeHg.

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Section: Discussionmentioning

confidence: 63%

Section: Animal Treatmentsmentioning

confidence: 99%

Section: Zuotai In Tibetan Medicinesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Zuotai (β-HgS)-containing 70 Wei Zhen-Zhu-Wan (Rannasangpei) differs from mercury chloride and methylmercury on hepatic cytochrome P450

Nie¹,

Xu²,

Lu³

et al. 2020

Preprint

Self Cite

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“…According to the Pharmacopoeia of China (2015), ten kinds of minerals are listed, including cinnabar (HgS) and realgar (As 4 S 4 ). Cinnabar (96% as HgS) has long been used for its sedative and hypnotic effects in TCM prescriptions [ 1 , 2 ], such as Zhusha Anshen Wan (ZSASW) [ 3 ], An-Gong-Niu-Huang Wan [ 4 ], etc. Although cinnabar has many special medical effects, the safety of cinnabar is becoming of more and more concern to the public due to the toxic effects caused by the high mercury content [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

1H-NMR-Based Metabonomics of the Protective Effect of Coptis chinensis and Berberine on Cinnabar-Induced Hepatotoxicity and Nephrotoxicity in Rats

Wang

Gao

et al. 2017

Molecules

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Coptis chinensis Franch has been used in Traditional Chinese Medicine (TCM) for treating infectious and inflammatory diseases for over two thousand years. Berberine (BN), an isoquinoline alkaloid, is the main component of Coptis chinensis. The pharmacological basis for its therapeutic effects, which include hepatoprotective effects on liver injuries, has been studied intensively, yet the therapy of liver injuries and underlying mechanism remain unclear. We investigated the detoxification mechanism of Coptis chinensis and berberine using metabolomics of urine and serum in the present study. After the treatment with Coptis chinensis and berberine, compared with the cinnabar group, Coptis chinensis and berberine can regulate the concentration of the endogenous metabolites. PLS-DA score plots demonstrated that the urine and serum metabolic profiles in rats of the Coptis chinensis and berberine groups were similar those of the control group, yet remarkably apart from the cinnabar group. The mechanism may be related to the endogenous metabolites including energy metabolism, amino acid metabolism and metabolism of intestinal flora in rats. Meanwhile, liver and kidney histopathology examinations and serum clinical chemistry analysis verified the experimental results of metabonomics.

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Pharmacology, Toxicology, and Therapeutic Effects of Metals and Minerals Used in Traditional Medicine

Perera¹,

Dahanayake²,

Diddeniya³

2023

Medical Geology

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Zuotai and HgS differ from HgCl2 and methyl mercury in Hg accumulation and toxicity in weanling and aged rats

Cited by 28 publications

References 35 publications

Zuotai (β-HgS)-containing 70 Wei Zhen-Zhu-Wan (Rannasangpei) differs from mercury chloride and methylmercury on hepatic cytochrome P450

Zuotai (β-HgS)-containing 70 Wei Zhen-Zhu-Wan (Rannasangpei) differs from mercury chloride and methylmercury on hepatic cytochrome P450

1H-NMR-Based Metabonomics of the Protective Effect of Coptis chinensis and Berberine on Cinnabar-Induced Hepatotoxicity and Nephrotoxicity in Rats

Pharmacology, Toxicology, and Therapeutic Effects of Metals and Minerals Used in Traditional Medicine

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