Association models for binding of molecules to nanostructures

Ahumada, Manuel; Lissi, Eduardo; Montagut, Ana Maria; Valenzuela-Henriquez, Francisco; Pacioni, Natalia L.; Alarcon, Emilio I.

doi:10.1039/c7an00288b

Cited by 40 publications

(29 citation statements)

References 89 publications

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“…A wide range of host molecules have been developed to provide specific binding of therapeutic molecules for nanoparticlebased drug delivery (18)(19)(20)(21). DNA and proteins are of particular interest, since they can be readily conjugated for attachment to gold nanoparticle surfaces, and their structures can be tailored for uptake of drug molecules in a host-guest manner (22)(23)(24).…”

mentioning

confidence: 99%

Near-infrared remotely triggered drug-release strategies for cancer treatment

Goodman

Neumann

Nørregaard

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Remotely controlled, localized drug delivery is highly desirable for potentially minimizing the systemic toxicity induced by the administration of typically hydrophobic chemotherapy drugs by conventional means. Nanoparticle-based drug delivery systems provide a highly promising approach for localized drug delivery, and are an emerging field of interest in cancer treatment. Here, we demonstrate near-IR light-triggered release of two drug molecules from both DNA-based and protein-based hosts that have been conjugated to near-infrared-absorbing Au nanoshells (SiO 2 core, Au shell), each forming a light-responsive drug delivery complex. We show that, depending upon the drug molecule, the type of host molecule, and the laser illumination method (continuous wave or pulsed laser), in vitro light-triggered release can be achieved with both types of nanoparticle-based complexes. Two breast cancer drugs, docetaxel and HER2-targeted lapatinib, were delivered to MDA-MB-231 and SKBR3 (overexpressing HER2) breast cancer cells and compared with release in noncancerous RAW 264.7 macrophage cells. Continuous wave laser-induced release of docetaxel from a nanoshell-based DNA host complex showed increased cell death, which also coincided with nonspecific cell death from photothermal heating. Using a femtosecond pulsed laser, lapatinib release from a nanoshell-based human serum albumin protein host complex resulted in increased cancerous cell death while noncancerous control cells were unaffected. Both methods provide spatially and temporally localized drug-release strategies that can facilitate high local concentrations of chemotherapy drugs deliverable at a specific treatment site over a specific time window, with the potential for greatly minimized side effects. chemotherapy | cancer | drug release | near-infrared | nanoparticle

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mentioning

confidence: 99%

Near-infrared remotely triggered drug-release strategies for cancer treatment

Goodman

Neumann

Nørregaard

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Although some differences in the values obtained by the different experimental techniques is apparent, they follow the same trend and are of similar order of magnitude reflecting the effect of the dendrimer size on the binding. It is well known that different experimental procedures and even differences in the treatment of the data lead to differences in the values of binding constants .…”

Section: Resultsmentioning

confidence: 99%

A Comparative Study on the Photophysical and Photochemical Properties of Dyes in the Presence of Low Generation Amino‐terminated Polyamidoamine Dendrimers

Grassano

Altamirano

Militello

et al. 2018

Photochem & Photobiology

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The photophysical and photochemical properties of the xanthene dyes mercurochrome (MCr) and eosin-Y (Eos); and the phenazine dye safranine-O (SF) are evaluated in the presence of amino-terminated polyamidoamine (PAMAM) dendrimers of low generations. The dendrimers produce a red shift in the UV-vis absorption spectra of the dyes, which increases with concentration and the size of the PAMAM molecule. The Stern-Volmer plots of fluorescence quenching for xanthenic dyes present a downward curvature. It is ascribed to a static mechanism involving a dye-dendrimer binding. A nonlinear fitting of the SV plots allows the calculation of the binding constants. For SF, the fluorescence is only slightly quenched by PAMAMs and the SV plots are linear. The binding constants are in the order K bind (SF) ( K bind (Eos) < K bind (MCr). The difference must be due to important specific structural effects. A decrease in the triplet lifetime and an increase in the absorption of the semireduced form of the dyes are observed in the presence of dendrimers. While for the two xanthene dyes, the rate constants reach the diffusional limit for G2 and G3, for SF they are one order of magnitude lower. This is explained by a different quenching mechanism of the two types of dyes.

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“…51 The binding constant of the TYMN-Zn complex formed in solution was calculated by using the standard Benesi-Hildebrand equation. [52][53][54] (2)…”

Section: Determination Of Binding Constantmentioning

confidence: 99%

A Simple Fluorescent Chemosensor for Detection of Zinc Ions in Some Real Samples and Intracellular Imaging in Living Cells

Aziz

Aboelhasan

Sayed

2020

J. Braz. Chem. Soc.

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A new designed fluorescent chemosensor TYMN ((E)-1-((thiazol-2-ylimino)methyl) naphthalen-2-ol) for highly sensitive and selective tracing of trace amount of Zn 2+ ions in some real samples was synthesized and characterized. The sensor TYMN can detect Zn 2+ ions via fluorescence enhancement with a high selectivity over a wide range of metal ions, especially Cd 2+. The sensor showed large fluorescence enhancement upon complexation with Zn 2+ and simultaneous color change from yellow to orange. The limit of detection was analyzed to be 0.0311 μM with a linear dynamic range 0.1-1.0 μM. The sensor could work in a pH span of 5.0-8.0. Based on the physicochemical and analytical methods like electrospray ionization (ESI)-mass, Job plot, 1 H nuclear magnetic resonance (NMR) and theoretical calculations, the detection mechanism for Zn 2+ was explained based on restriction of internal charge transfer (ICT) mechanism. TYMN sensor was potentially utilized for Zn 2+ ions concentration evaluation in some real samples. Fluorescence microscopy experiments revealed that probe TYMN may have application as a fluorophore to detect the Zn 2+ in living cells.

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Association models for binding of molecules to nanostructures

Cited by 40 publications

References 89 publications

Near-infrared remotely triggered drug-release strategies for cancer treatment

Near-infrared remotely triggered drug-release strategies for cancer treatment

A Comparative Study on the Photophysical and Photochemical Properties of Dyes in the Presence of Low Generation Amino‐terminated Polyamidoamine Dendrimers

A Simple Fluorescent Chemosensor for Detection of Zinc Ions in Some Real Samples and Intracellular Imaging in Living Cells

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