Overexpression of a SNARE protein AtBS14b alters BR response in Arabidopsis

Luo, Hua; Ye, Hong Bo; Xuan, Yuan; Yao, Da Nian

doi:10.1186/s40529-014-0055-5

Cited by 6 publications

(7 citation statements)

References 22 publications

(34 reference statements)

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“…Overexpression of the AtBS14b caused insensitivity to exogenously applied BRs. AtBS14b directly interacts with the Membrane Steroid-Binding Protein1 (MSBP1) at vesicular compartments, but not with BRI1 or BAK1 [ 96 ]. The MSBP1 protein is localized in the plasma membrane and endosomes, and is able to bind the BR ligand.…”

Section: The Br Signalosome—an Updatementioning

confidence: 99%

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Crosstalk of the Brassinosteroid Signalosome with Phytohormonal and Stress Signaling Components Maintains a Balance between the Processes of Growth and Stress Tolerance

Gruszka

2018

IJMS

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Brassinosteroids (BRs) are a class of phytohormones, which regulate various processes during plant life cycle. Intensive studies conducted with genetic, physiological and molecular approaches allowed identification of various components participating in the BR signaling—from the ligand perception, through cytoplasmic signal transduction, up to the BR-dependent gene expression, which is regulated by transcription factors and chromatin modifying enzymes. The identification of new components of the BR signaling is an ongoing process, however an emerging view of the BR signalosome indicates that this process is interconnected at various stages with other metabolic pathways. The signaling crosstalk is mediated by the BR signaling proteins, which function as components of the transmembrane BR receptor, by a cytoplasmic kinase playing a role of the major negative regulator of the BR signaling, and by the transcription factors, which regulate the BR-dependent gene expression and form a complicated regulatory system. This molecular network of interdependencies allows a balance in homeostasis of various phytohormones to be maintained. Moreover, the components of the BR signalosome interact with factors regulating plant reactions to environmental cues and stress conditions. This intricate network of interactions enables a rapid adaptation of plant metabolism to constantly changing environmental conditions.

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Section: The Br Signalosome—an Updatementioning

confidence: 99%

“…Transcription of the AtBS14b gene is down-regulated by BR in the BRI1-mediated process. AtBS14b acts via interacting with MSBP1 in the trans-Golgi network to enhance transport of MSBP1 to plasma membrane and its interaction with BAK1 [ 96 ] ( Figure 1 ).…”

Section: The Br Signalosome—an Updatementioning

confidence: 99%

Crosstalk of the Brassinosteroid Signalosome with Phytohormonal and Stress Signaling Components Maintains a Balance between the Processes of Growth and Stress Tolerance

Gruszka

2018

IJMS

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“…They showed longer petioles, larger rosette diameters, less rosette leaves and slender shoots (Fig 5A and 5C and S2 Fig). We further observed the elongated hypocotyls in all these over-expressing lines (Fig 5B and 5D) [14]. Yet, they were all sensitive to BRZ (brassinozole) that specifically inhibits BR biosynthesis (Fig 5D and S3 Fig).…”

Section: Resultsmentioning

confidence: 82%

Functional study of the brassinosteroid biosynthetic genes from Selagnella moellendorfii in Arabidopsis

Zheng

Bai

et al. 2019

PLoS ONE

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Brassinosteroids (BRs) are essential hormones for plant growth and development. Enzymes DET2 and CYP90 family are responsible for BR biosynthesis in seed plants. Yet, their roles in non-seed plants are unknown. Here, we report the first functional study of DET2 and all 4 CYP90 genes isolated from Selaginella moellendorfii . Sm89026 ( SmCPD ) belonged to a clade with CYP90A1 ( CPD ) and CYP90B1 ( DWF4 ) while Sm182839 , Sm233379 and Sm157387 formed a distinct clade with CYP90C1 ( ROT3 ) and CYP90D1 . SmDET2 , SmCPD and Sm157387 were highly expressed in both leaves and strobili while Sm233379 was only highly expressed in the leaves but not strobili, implying their differential functions in a tissue-specific manner in S . moellendorfii . We showed that only SmDET2 and SmCPD completely rescued Arabidopsis det2 and cpd mutant phenotypes, respectively, suggestive of their conserved BR biosynthetic functions. However, neither SmCPD nor other CYP90 genes rescued any other cyp90 mutants. Yet overexpression of Sm233379 altered plant fertility and BR response, which means that Sm233379 is not an ortholog of any CYP90 genes in Arabidopsis but appears to have a BR function in the S . moellendorfii leaves. This function is likely turned off during the development of the strobili. Our results suggest a dramatic functional divergence of CYP90 family in the non-seed plants. While some of them are functionally similar to that of seed plants, the others may be functionally distinct from that of seed plants, shedding light for future exploration.

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“…The YFP signals generated by GsCBRLK/GsBET11a co‐expression were mainly observed on the PM, as well as on some Golgi‐like punctate structures (Figure 1c). GsBET11a is homologous to AtBET11 (Figure 2a), which was suggested to be a Golgi‐located Qc‐SNARE (Bubeck et al ., 2008; Bolaños‐Villegas et al ., 2015), and AtBET12 was targeted at Golgi/TGN membranes (Bolaños‐Villegas et al ., 2015; Chung et al ., 2018) as well as the vesicular compartments surrounding the PM (Zhu et al ., 2014). In the present study, we showed that GsBET11a appeared in the Golgi/TGN‐like punctate structures and the PM (Figure 1e; Figure S1b), and this enabled its interaction with the PM‐located GsCBRLK (Yang et al ., 2010).…”

Section: Discussionmentioning

confidence: 99%

Wild soybean SNARE proteins BET1s mediate the subcellular localization of the cytoplasmic receptor‐like kinases CRCK1s to modulate salt stress responses

et al. 2020

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Summary Plants have evolved numerous receptor‐like kinases (RLKs) that modulate environmental stress responses. However, little is known regarding soybean (Glycine max) RLKs. We have previously identified that Glycine soja Ca2+/CAM‐binding RLK (GsCBRLK) is involved in salt tolerance. Here, we report that soluble NSF attachment protein receptor proteins BET1s mediate subcellular localization of calmodulin‐binding receptor‐like cytoplasmic kinases CRCK1s to modulate salt stress responses. Direct interaction between GsCBRLK and GsBET11a was initially identified via yeast two‐hybrid and bimolecular fluorescence complementation assays. Further analysis demonstrated conserved interaction between BET1s and CRCK1s. GsCBRLK interacted with all BET1 proteins in wild soybean (Glycine soja) and Arabidopsis, and GsBET11a strongly associated with GsCRCK1a–1d, but slightly with AtCRCK1. In addition, GsBET11a interacted with GsCBRLK via its C‐terminal transmembrane domain (TMD), where the entire TMD, not the sequence, was critical for the interaction. Moreover, the N‐terminal variable domain (VD) of GsCBRLK was responsible for interacting with GsBET11a, and the intensity of interaction between GsCBRLK/AtCRCK1 and GsBET11a was dependent on VD. Furthermore, GsBET11a was able to mediate the GsCBRLK subcellular localization via direct interaction with VD. Additionally, knockout of AtBET11 or AtBET12 individually did not alter GsCBRLK localization, while GsBET11a expression caused partial internalization of GsCBRLK from the plasma membrane (PM). We further suggest the necessity of GsCBRLK VD for its PM localization via N‐terminal truncation assays. Finally, GsBET11a was shown to confer enhanced salt stress tolerance when overexpressed in Arabidopsis and soybean. These results revealed the conserved and direct interaction between BET1s and CRCK1s, and suggested their involvement in salt stress responses.

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Overexpression of a SNARE protein AtBS14b alters BR response in Arabidopsis

Cited by 6 publications

References 22 publications

Crosstalk of the Brassinosteroid Signalosome with Phytohormonal and Stress Signaling Components Maintains a Balance between the Processes of Growth and Stress Tolerance

Crosstalk of the Brassinosteroid Signalosome with Phytohormonal and Stress Signaling Components Maintains a Balance between the Processes of Growth and Stress Tolerance

Functional study of the brassinosteroid biosynthetic genes from Selagnella moellendorfii in Arabidopsis

Wild soybean SNARE proteins BET1s mediate the subcellular localization of the cytoplasmic receptor‐like kinases CRCK1s to modulate salt stress responses

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