Low-level laser treatment stimulates hair growth via upregulating Wnt10b and β-catenin expression in C3H/HeJ mice

Abstract

This study was conducted in order to evaluate the role of low-level laser treatment (LLLT) in hair growth in C3H/HeJ mice. Healthy C57BL/6 mice were randomly divided into two groups: with and without low-level laser treatment. The skin color of each mouse was observed each day. Skin samples were collected for H&E, immunofluorescence, PCR, and western blot analysis, to observe the morphology of hair follicles and detect the expression levels of Wnt10b and β-catenin. Observation of skin color demonstrated that black pigmentation started significantly earlier in the laser group than in the control group. Hair follicle number in both groups showed no difference; however, the hair follicle length presented a significant difference. Wnt10b protein was detected on the second day in hair matrix cells in the LLLT group but not in the control group. PCR and western blot results both illustrated that expression of Wnt10b and β-catenin was significantly higher in the LLLT group than in the control group. Our study illustrated that low-level laser treatment can promote hair regrowth by inducing anagen phase of hair follicles via initiating the Wnt10b/β-catenin pathway.

Single‐cell transcriptome analysis of fractional CO2 laser efficiency in treating a mouse model of alopecia

“…Comprehensive single-cell gene expression analysis revealed that Ccr2 gene expression was elevated in recruited inflammatory macrophages. Although several reports have demonstrated increased Wnt10b-β-catenin and VEGF expression after fractional laser irradiation in a mouse alopecia model, 37,38,44 we did not observe these differences in our single-cell RNA-seq analysis. Other reports have demonstrated that macrophages and the CCR2-TNFa signaling axis are involved in hair cycle progression, while dermal macrophages maintain hair follicle quiescence.…”

DNA methylation and histone acetylation are involved in Wnt10b expression during the secondary hair follicle cycle in Angora rabbits

“…Our findings indicate that Wnt10b plays an important role during the SHF cycle of Angora rabbits. It was reported that Wnt10b can promote HF regeneration via inducing their switch from telogen to anagen (Zhang, Liu, et al., 2017). Hence, we infer that Wnt10b is a critical activator in cyclic transition of hair.…”

“…It was reported that Wnt10b could promote HF development and regeneration by activating the canonical Wnt/β‐catenin signalling pathway (Li et al., 2013; Ouji et al., 2006). This may be achieved by inducing the switch from telogen phase to anagen phase (Zhang, Liu, et al., 2017). However, few studies of Wnt10b in rabbit HF cycling have been conducted (Li et al., 2013; Tosti et al., 2016).…”

Dermatology today and tomorrow: from symptom control to targeted therapy

“…Previous studies show increased human hair growth with valproic acid and, another Wnt/β‐catenin activator, methyl vanillate . Low‐level laser therapy also has been shown to stimulate hair growth by activating Wnt/β‐catenin signalling . Thus, activation of the WNT/β‐catenin pathway appears a promising potential therapeutic target in AGA.…”