Expressions of matrix metalloproteinases 1 and 3 and their tissue inhibitors in the conjunctival tissue and fibroblasts cultured from conjunctivochalasis

Xiang, Minhong; Zhang, Xingru; Zhang, Zhen-Yong; Li, Qingsong; Wang, Hanmin; Han, Zhu-mei; Zhou, Huan-ming; Jia, Yuan-Ling; Chen, Xing‐Xing

doi:10.18240/ijo.2017.04.09

Cited by 10 publications

(7 citation statements)

References 11 publications

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“…While CCH has been a recognized pathology since 1908, only during the last several years have there been attempts to investigate the condition and its role in the pathogenesis of dry eye syndrome [ 7 , 8 , 9 ].…”

Section: Discussionmentioning

confidence: 99%

The Location of Conjunctivochalasis and Its Clinical Correlation with the Severity of Dry Eye Symptoms

2018

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Background: We aimed to investigate the clinical importance of conjunctivochalasis (CCH) and, further, to implement a new CCH classification system. Methods: 60 eyes of patients with whom, upon clinical examination, CCH was diagnosed were investigated for the presence of symptoms and signs characteristic of dry eye. The eyes were grouped based on two stages of severity, Stage 1 (minimal/mild) and Stage 2 (medium/severe), for each nasal, middle, and temporal position, and on the extent of CCH folds in each site. Results: In 40 (66.6%) out of 60 eyes, symptoms and signs of CCH were manifest: pain in 25 (41.6%), epiphora in 25 (41.6%), and lacrimal punctum obstruction from conjunctival folds in 22 (36.6%) eyes. Depending on the position of CCH, a greater percentage of symptoms appeared in Stage 2 in the nasal position (78.9%), followed by middle (68.7%) and temporal positions (60%). When TBUT values were compared, statistically significant differences were found proportional to grading (p < 0.001) and position (nasal more severe than temporal, p < 0.001), and such differences were also found when TBUT values of all eyes were compared with those of symptomatic eyes (p = 0.01) and with those of symptom-free eyes (p = 0.002). Conclusions: CCH is a rather frequent and commonly unrecognized condition that should always be considered in differential diagnoses of dry eye.

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Section: Discussionmentioning

confidence: 99%

The Location of Conjunctivochalasis and Its Clinical Correlation with the Severity of Dry Eye Symptoms

2018

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“…A recent transcriptome analysis of human conjunctiva with CCh demonstrated that reduced gene expression could cause decreased cell viability, which is related to the aging process 26 . The second is OS inflammation, which induces elastic fiber breakdown by destructive fibroblasts and infiltrated cells, resulting in initiating or accelerating CCh 13 , 14 , 27 – 29 . Proinflammatory proteins also have been detected in tears of CCh patients 15 , 30 – 32 .…”

Section: Discussionmentioning

confidence: 99%

“…CCh is often found between the globe and lower eyelid, but in severe cases can be observed in the superior and even the whole bulbar conjunctiva 2 , 12 . CCh has been thought to be caused by elastic fiber degradation involving inflammatory factors or mechanical interference with conjunctival lymphatic circulation 13 – 15 . Recently, CCh is considered to be one of the friction-related diseases (FRD), which also include superior limbic keratoconjunctivitis and lid wiper epitheliopathy 16 .…”

Section: Introductionmentioning

confidence: 99%

The correction of conjunctivochalasis using high-frequency radiowave electrosurgery improves dry eye disease

Seong

Lee

et al. 2021

Sci Rep

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We aimed to determine the clinical impact of conjunctivochalasis (CCh) and its correction using high-frequency radiowave electrosurgery (HFR-ES), for signs and symptoms of dry eye disease (DED). Forty patients diagnosed with symptomatic CCh were prospectively enrolled. As a result, patients with CCh had moderate to severe DED and most of them exhibited meibomian gland dysfunction (MGD). Corneo-conjunctival fluorescein staining score (CFS) and all lid-parallel-conjunctival-folds scores (LIPCOFs) were positively correlated. Nasal LIPCOF significantly correlated with symptoms and tear volume. Central, temporal, and total LIPCOF significantly correlated with MG loss, MGD stage, and lipid layer thickness. Independent significant factors associated with total LIPCOF included CFS, tear break-up time, and MGD stage. One month following HFR-ES, CCh was completely resolved in all cases. Patient age and preoperative nasal LIPCOF were determinants of outcomes associated with postoperative improvements in symptoms. Ocular surface parameters significantly improved, but MGD-related signs did not. Collectively, CCh associated with MGD severity deteriorates not only tear film stability and reservoir capacity, leading to DED exacerbation. Therefore, CCh should be corrected in patients with DED and MGD. Younger patients with nasal CCh are likely to experience more symptomatic relief after HFR-ES. Particularly, management for MGD should be maintained after CCh correction.

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“…On the other hand, these cytokines may locally induce matrix metalloproteinase (MMPs) or serine protease activity, which may directly or indirectly modulate ECM protein production or their destruction [4,19,20]. Inflammation has also been shown to alter the expression of MMPs or serine proteases in the conjunctival tissue, which may initiate collagenous fibre melting [21]. In our study, we found that the presence of collagen type I does not significantly change urokinase-type plasminogen activator (uPA) and its receptor (uPAR) production in the co-culture models.…”

Section: Discussionmentioning

confidence: 99%

The role of collagen in co-cultures of human normal corneal and conjunctival cells

et al. 2019

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BaCkground: Intermediate interactions between corneal and conjunctival epithelial cells play an important role in the process of correct vision. The goal of this paper was to establish whether the presence or absence of collagen type I changes paracrine interactions between corneal and conjunctival epithelial cells. Material and Methods: Cultures of human corneal and conjunctival epithelial cells were used in the study. The ELISA quantitative analysis of interleukin 1b (IL-1b), interleukin 6 (IL-6), urokinase-type plasminogen activator (uPA), and uPA receptor (uPAR), assessment of the type of interactions between cells, as well as correlations between tested parameters were performed. results: The presence of collagen type I changed the quantitative production of IL-1b and IL-6 by the examined cells in the co-culture system. It did not affect the level of released uPA and uPAR. The presence or absence of collagen also changed the relationship between the cells, which were evaluated in relation to changes in the level of released cytokines. ConClusions: Different levels of collagen type I constituting a component of extracellular matrix proteins significantly affect and regulate the indirect interactions between human corneal and conjunctival epithelial cells.

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Expressions of matrix metalloproteinases 1 and 3 and their tissue inhibitors in the conjunctival tissue and fibroblasts cultured from conjunctivochalasis

Abstract: The overexpression of MMP-1 and MMP-3 in conjunctival tissue and fibroblasts may play an important role in the pathogenesis and development of CCh.

Cited by 10 publications

References 11 publications

The Location of Conjunctivochalasis and Its Clinical Correlation with the Severity of Dry Eye Symptoms

The Location of Conjunctivochalasis and Its Clinical Correlation with the Severity of Dry Eye Symptoms

The correction of conjunctivochalasis using high-frequency radiowave electrosurgery improves dry eye disease

The role of collagen in co-cultures of human normal corneal and conjunctival cells

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