Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits

Ichikawa, Yukio; Zemljic-Harpf, Alice; Zhang, Zheng; McKirnan, M. Dan; Manso, Ana Maria; Ross, Robert S.; Hammond, H. Kirk; Patel, Hemal H.; Roth, David M.

doi:10.1371/journal.pone.0177660

Cited by 14 publications

(9 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our results, Cav-3 expression was increased 1.6-fold in DDC-fed TG neg hearts compared with chow-fed TG neg hearts. This result is consistent with previous reports that structural caveolae and Cav-3 protein were both increased in a rabbit model of daunorubicin cardiotoxicity (11). Therefore, the increase of Cav-3 in the hearts under liver cirrhosis might be a compensatory increase, and further studies for the protective mechanism of Cav-3 OE are needed.…”

Section: G538supporting

confidence: 92%

“…The chow-fed Cav-3 OE hearts showed approximately three times higher Cav-3 protein expression than chow-fed TG neg hearts (P Ͻ 0.001). Cav-3 expression was increased 1.6-fold in DDC-fed TG neg hearts compared with chow-fed TG neg hearts (P ϭ 0.041), which is similar to our previous reports of increased Cav-3 expression in daunorubicin-treated rabbit hearts (11). There were no differences in fibrosis of the heart between the four groups (Fig.…”

Section: G535supporting

confidence: 90%

See 1 more Smart Citation

Protective role of cardiac-specific overexpression of caveolin-3 in cirrhotic cardiomyopathy

Kim

Schilling

et al. 2020

American Journal of Physiology-Gastrointestinal and Liver Physi

Self Cite

View full text Add to dashboard Cite

Cirrhotic cardiomyopathy is a clinical syndrome in patients with liver cirrhosis characterized by blunted cardiac contractile responses to stress and/or heart rate-corrected QT (QTc) interval prolongation. Caveolin-3 (Cav-3) plays a critical role in cardiac protection and is an emerging therapeutic target for heart disease. We investigated the protective role of cardiac-specific overexpression (OE) of Cav-3 in cirrhotic cardiomyopathy. Biliary fibrosis was induced in male Cav-3 OE mice and transgene negative (TGneg) littermates by feeding a diet containing 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC; 0.1%) for 3 wk. Liver pathology and blood chemistries were assessed, and stress echocardiography, telemetry, and isolated heart perfusion studies to assess adrenergic responsiveness were performed. Cav-3 OE mice showed a similar degree of hyperdynamic contractility, pulmonary hypertension, and QTc interval prolongation as TGneg mice after 3 wk of DDC diet. Blunted systolic responses were shown in both DDC-fed Cav-3 OE and TGneg hearts after in vivo isoproterenol challenge. However, QTc interval prolongation after in vivo isoproterenol challenge was significantly less in DDC-fed Cav-3 OE hearts compared with DDC-fed TGneg hearts. In ex vivo perfused hearts, where circulatory factors are absent, isoproterenol challenge showed hearts from DDC-fed Cav-3 OE mice had better cardiac contractility and relaxation compared with DDC-fed TGneg hearts. Although Cav-3 OE in the heart did not prevent cardiac alterations in DDC-induced biliary fibrosis, cardiac expression of Cav-3 reduced QTc interval prolongation after adrenergic stimulation in cirrhosis. NEW & NOTEWORTHY Prevalence of cirrhotic cardiomyopathy is up to 50% in cirrhotic patients, and liver transplantation is the only treatment. However, cirrhotic cardiomyopathy is associated with perioperative morbidity and mortality after liver transplantation; therefore, management of cirrhotic cardiomyopathy is crucial for successful liver transplantation. This study shows cardiac myocyte specific overexpression of caveolin-3 (Cav-3) provides better cardiac contractile responses and less corrected QT prolongation during adrenergic stress in a cirrhotic cardiomyopathy model, suggesting beneficial effects of Cav-3 expression in cirrhotic cardiomyopathy.

show abstract

Section: G538supporting

confidence: 92%

Section: G535supporting

confidence: 90%

Protective role of cardiac-specific overexpression of caveolin-3 in cirrhotic cardiomyopathy

Kim

Schilling

et al. 2020

American Journal of Physiology-Gastrointestinal and Liver Physi

Self Cite

View full text Add to dashboard Cite

show abstract

“…Given that rabbit models of human heart disease have similarities to human physiology and are practical alternatives to larger mammals (Pogwizd and Bers, 2008), we used four groups of blood plasma obtained from previous studies in rabbit heart failure Models (Ichikawa et al, 2017;McKirnan et al, 2019). Brie y, in the MI experiment, New Zealand White rabbits underwent thoracotomy wherein a cryo-probe cooled with liquid N 2 was used to induce transmural MI by freezing anterior-lateral left ventricular (LV) free wall for 3 min.…”

Section: Blood Plasma Preparationmentioning

confidence: 99%

Impact of blood factors on endothelial cell metabolism and function in two diverse heart failure models

Song

Leem

Dhanani

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Role of blood-based factors in development and progression of heart failure (HF) is poorly characterized. Blood contains myriads of factors released during pathophysiological states that may impact cellular function and provide mechanistic insights to HF management. In this study, we tested effects of blood from two distinct HF models on cardiac metabolism and identified possible cellular targets of the effects. Methods: Blood plasma was obtained from daunorubicin- and myocardial infarction-induced HF rabbits (Dauno-HF and MI-HF) and their controls (Dauno-Control and MI-Control). Effects of plasma on bioenergetics of myocardial tissue from healthy mice and cellular cardiac components; myoblasts, fibroblasts, macrophages, and endothelial cells were assessed using high-resolution respirometry and Seahorse flux analyzer. Since endothelial cell respiration was profoundly affected by HF plasma, effects of plasma on endothelial cell barrier function and death were further evaluated. Western-blot analysis and electron microscopic assessment were performed to detect alterations in mitochondrial proteins and morphology. Results: Brief exposure to HF plasma significantly decreased cardiac tissue respiration. Endothelial cell respiration was most impacted by exposure to HF plasma. Endothelial cell monolayer integrity was significantly decreased by incubation with Dauno-HF plasma. Apoptosis and necrosis were significantly increased in cells incubated with Dauno-HF plasma for 24 h. Significant down-regulation of VDAC-1, Tom20, and MFF in cells exposed to Dauno-HF plasma and mitochondrial Stat3 and MFF in cells exposed to MI-HF plasma were observed. Mitochondrial structure was disrupted in cells exposed to HF plasma.Conclusions: These findings indicate that endothelial cells and mitochondrial structure and function may be a primary target where HF pathology manifests and accelerates. High-throughput blood-based screening of HF may provide innovative ways to advance disease diagnosis and management.

show abstract

“…Препараты вводят внутримышечно, реже подкожно [2-4, 6, 17-21]. Некоторые авторы применяют ингаляционный масочный наркоз с использованием 1,0-1,5% изофлурана (галогенизированный анестетик) -в комбинации с мидазоламом (2 мг/ кг) [13,22]. Возможна также эндотрахеальная интубация и ингаляционный наркоз 1,8% изофлураном после вводной анестезии кетамином (10 мг/ кг) и мидазоламом (0,1 мг/кг) [3].…”

Section: подготовка к исследованиюunclassified

Echocardiography in laboratory rabbits

Изможерова

Charkin

Маркова

et al. 2022

jour

View full text Add to dashboard Cite

Introduction. Rabbits are used as convenient models for studying drug cardiotoxicity. Echocardiography is one of the most informative non-invasive methods of assessing the cardiovascular system function. In literature, there is no clearly formulated protocol for heart ultrasound examination in rabbits. Purpose was the systematization of literature data on echocardiography techniques in rabbits.Materials and methods. The sources search involved the Scientific Electronic Library eLIBRARY.RU and the National Library of Medicine information base (PubMed.gov). The search covered the 15-year period. Original or review articles containing a detailed description of the echocardiography technique were included.Results and discussion. For animals anesthesia before the study, various drugs combinations based on ketamine, xylazine, medetomidine, zolazepam, tiletamine are used. In rabbits, it is possible to use M-, B-, Doppler echocardiography modes in a wide frequency range (5 – 12 MHz). Parasternal longitudinal, parasternal transverse and apical ultrasound approaches are applied. The parameters of the transmitral blood flow (peak A, peak E, ratio A/E, peak E', the magnitude of regurgitation), as well as the contractile function of the left ventricle (end systolic and diastolic volumes and sizes, stroke volume, cardiac output, shortening fraction) are subject to assessment. The Teicholz and Simpson methods can be used to calculate the ejection fraction. The literature under survey coveres listed parameters of normal values for various ages’ rabbits.Conclusion. Echocardiography is a relatively affordable non-invasive method for studying the state of cardiovascular system in rabbits in an experiment. The method can be used to analyze myocardium size, left ventricle contractile function, and assess the state of heart valvular apparatus. At present, normal values of echocardiographic parameters in rabbits have been experimentally established, which adds to the informative value of the method. Qualitative study performance requires special preparation, including careful animal anesthesia, the choice of the approaches and modes used.

show abstract

Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits

Cited by 14 publications

References 50 publications

Protective role of cardiac-specific overexpression of caveolin-3 in cirrhotic cardiomyopathy

Protective role of cardiac-specific overexpression of caveolin-3 in cirrhotic cardiomyopathy

Impact of blood factors on endothelial cell metabolism and function in two diverse heart failure models

Echocardiography in laboratory rabbits

Contact Info

Product

Resources

About