Effects of in situ exposure to tritiated natural environments: A multi-biomarker approach using the fathead minnow, Pimephales promelas

Gagnaire, Béatrice; Adam-Guillermin, Christelle; Festarini, Amy; Cavalié, Isabelle; Della-Vedova, Claire; Shultz, Carmen; Kim, S.B.; Ikert, Heather; Dubois, Cécile; Walsh, Stephanie; Farrow, F.; Beaton, Danielle; Tan, Elizabeth; Wen, Kendall; Stuart, Marilyne

doi:10.1016/j.scitotenv.2017.04.210

Cited by 23 publications

(36 citation statements)

References 72 publications

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“…Results showed that, comparing to their respective controls, no significant differences were found for both dose rates. After an exposure to tritium at much lower activities than in the present study, no difference between control and contaminated organisms were neither found in fish cells and in situ exposed fathead minnows (Gagnaire et al, 2017;Stuart et al, 2016) using gamma-H2AX foci detection. At the same stage, i.e.…”

Section: Gamma-h2ax Foci Detectioncontrasting

confidence: 87%

“…The effects of HTO exposure on DNA damages were already investigated using a wide range of species as mollusc (Hagger et al, 2005;Jha et al, 2005), aquatic vertebrates (Gagnaire et al, 2017;Suyama et al, 1981) or fish cell culture (Stuart et al, 2016). In the present study, we focused on DNA double strand breaks in 24 hpf embryos using an immunostaining method to assess the number of gamma-H2AX foci, an early marker of DNA double strand break repair (Rogakou et al, 1998).…”

Section: Gamma-h2ax Foci Detectionmentioning

confidence: 94%

“…For those organisms, studies ranged from in vitro (Stuart et al, 2016) to in situ (Gagnaire et al, 2017) through laboratory studies mainly focusing on developmental and reproductive effects (Adam-Guillermin et al, 2012). Despite the wide range of species in which effects of HTO exposure were studied, there is still a lack of knowledge of tritium modes of action at the molecular scale, the transgenerational effects, the adaptation or the behavior of organisms (Adam-Guillermin et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

et al. 2020

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Distributed under a Creative Commons Attribution-NonCommercial-NoDerivatives| 4.0 International License

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Section: Gamma-h2ax Foci Detectioncontrasting

confidence: 87%

Section: Gamma-h2ax Foci Detectionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

et al. 2020

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“…The genotoxicity of various contaminants (e.g. metals, nanoparticles, insecticides and radionuclides) using induction of γ-H2AX foci as a biomarker has been displayed in zebrafish (Danio rerio) derived ZF4 cells, adult zebrafish liver tissue and retina, and fathead minnow (Pimephales promelas) juveniles [51][52][53][54][55]. Concentration-dependent DNA damage in ZF4 cells was evident following aluminium (10-100 µM) and cadmium (1-100 µM) exposures [53].…”

Section: Introductionmentioning

confidence: 99%

Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers

Vernon

Jha

2019

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Determination of relative sensitivity of biota following exposures to contaminants including metals is important for environmental protection. Copper (Cu), although biologically essential can be highly toxic to biota if present at higher concentrations in the natural environment. Given its ubiquitous presence within coastal and inland water bodies, we compared Cu-induced genotoxicity in two ecologically important mussel species, the freshwater Dreissena polymorpha (DP) and marine Mytilus galloprovincialis (MG), along with its tissue specific accumulation. Novel biomarker in terms of induction of gamma H2AX (γ-H2AX) foci, along with comet assay and induction of micronuclei (MN) were used to determine DNA damage response (DDR) in these two species following exposure to a range of Cu concentrations (18, 32, 56 µg L-1) for 10 days. Concentration-dependent increases in Cu concentration in gill tissue, as determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), were paralleled by a greater degree of genotoxicity. An induction of γ-H2AX foci was present in all Cu exposure concentrations, proving this technique to be a sensitive and suitable biomarker of genotoxicity in bivalves. The multibiomarker approach adopted here suggests firstly that in parallel with MG, which is widely used to assess the health of marine and coastal environment, DP is also suitable representative of inland water bodies, and that there is a similar mechanism of action for the induction of genotoxicity between the two species, following exposure to Cu. Secondly, for genotoxicity assessment a battery of responses could simultaneously be assessed in these two bivalve species. Finally, for adequate protection of the environment it is vital to adopt a multi-biomarker, multi-species approach to determine adverse biological effects to gain a holistic understanding of the real threat posed by contaminants to hydrosphere.

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“…Exchanges of HTO between water and organisms are rapid: maximal dose rates were obtained for 24-h eggs. Several studies showed that tritium accumulated in adult organisms 7: Relative expression of genes (normalized to reference gene ef1) compared to control in larvae contaminated during 10 d at 10 4 , 10 5 , and 10 6 Bq/mL of tritiated water (n = 9) a 4 dpf 7 dpf 10 dpf 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL ache equilibrates with HTO concentration of the water and that the fraction of tritium bound to organic matter equilibrates with the concentration of HTO internalized (Galeriu and Melintescu 2011;Kim and Korolevych 2013;Melintescu et al 2015;Kim et al 2015Kim et al , 2019Gagnaire et al 2017Gagnaire et al , 2020. A companion paper study presented in detail the discussion about internalization results obtained after tritium contamination for 2 stages (24-h eggs and 4-dpf larvae) and 2 HTO concentrations (10 5 and 10 6 Bq/mL; Arcanjo et al 2019).…”

Section: Dose Rates Calculated For Htomentioning

confidence: 99%

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Gagnaire

Arcanjo

Cavalié

et al. 2020

Enviro Toxic and Chemistry

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Tritium, a radioactive isotope of hydrogen of natural and anthropogenic origin, is ubiquitously present in the environment. Effluents of nuclear centers of production are significant anthropogenic sources. With the upcoming project of thermonuclear fusion, tritium releases in the environment may increase. It is therefore important to characterize the ecological risk linked to tritium. The effects of tritiated water (HTO) were therefore studied in zebrafish larvae exposed for 10 d to different dose rates, 1.1 × 102, 4.1 × 102, and 3.8 × 103 µGy/h for larvae corresponding, respectively, to a water contamination of 104, 105, and 106 Bq/mL of HTO. Those dose rates were higher than 10 μGy/h, which is the threshold recommended to start monitoring ecosystems where radiological contaminants are present. Mortality, embryo‐larval development, immune toxicity, genotoxicity, neurotoxicity, and alterations of tissues were investigated. The results showed that HTO exposure induced DNA damage and reactive oxygen species production and modulated the expression of genes involved in detoxification processes. Moreover, modifications of the muscular tissues (degradation of myofibrils at 4 d post fertilization and disorganization of mitochondria at later stages) were observed. The results differed with HTO dose rates and with developmental stages. These results will drive future research for the development of new HTO‐sensitive biomarkers and will allow us to progress in the characterization of the modes of action of tritium in fish. Environ Toxicol Chem 2020;39:648–658. © 2019 SETAC

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Effects of in situ exposure to tritiated natural environments: A multi-biomarker approach using the fathead minnow, Pimephales promelas

Cited by 23 publications

References 72 publications

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

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