An Efficient Method for the Preparative Isolation and Purification of Flavonoids from Leaves of Crataegus pinnatifida by HSCCC and Pre-HPLC

Abstract

In this work, flavonoid fraction from the leaves of Crataegus pinnatifida was separated into its seven main constituents using a combination of HSCCC coupled with pre-HPLC. In the first step, the total flavonoid extract was subjected to HSCCC with a two-solvent system of chloroform/methanol/water/n-butanol (4:3:2:1.5, v/v), yielding four pure compounds, namely (–)-epicatechin (1), quercetin-3-O-(2,6-di-α-l-rhamnopyranosyl)-β-d-galactopyranoside (2), 4′′-O-glucosylvitexin (3) and 2′′-O-rhamnosylvitexin (4) as well as a mixture of three further flavonoids. An extrusion mode was used to rapidly separate quercetin-3-O-(2,6-di-α-l-rhamnopyranosyl)-β-d-galactopyranoside with a big KD-value. In the second step, the mixture that resulted from HSCCC was separated by pre-HPLC, resulting in three pure compounds including: vitexin (5), hyperoside (6) and isoquercitrin (7). The purities of the isolated compounds were established to be over 98%, as determined by HPLC. The structures of these seven flavonoids were elucidated by ESI-MS and NMR spectroscopic analyses.

Transcriptome Analysis Reveals Biosynthesis of Important Bioactive Constituents and Mechanism of Stem Formation of Dendrobium huoshanense

“…Flavonoids are known for their high diversity, wide distribution, and multiple biological functions 32 . A previous study has shown that the leaves of D. officinale contained high contents of flavonoids and showed strong DPPH radical scavenging activities 30 , so that the leaves could be developed as antioxidant sources for medicinal and health food resources.…”

“…AP2-like transcription factors belong to the large plant-specific superfamily APETALA2 (AP2), which take part in various regulatory pathways and act in multiple stages and tissues during plant development 27 . Some members of the AP2 gene family could affect internode length, and the overexpression of an AP2-like gene in Arabidopsis promotes plant height 32,38 . Four unigenes expressed highly in stems and ten unigenes expressed highly in roots.…”

Development of a method to screen and isolate bioactive constituents from Stellera chamaejasme by ultrafiltration and liquid chromatography combined with semi‐preparative high‐performance liquid chromatography and high‐speed counter current chromatography

“…The choice of the solvent system is critical in HSCCC. According to a previous study, the most suitable K value range for HSCCC is 0.5-2.0 [21]. If a proper solvent system is not selected, the separation time will be long, the separation efficiency will be low, and organic solvent usage will be high.…”

Putative Genes Identified in Alkaloids Biosynthesis in Dendrobium officinale by Correlating the Contents of Main Bioactive Metabolites with Genes Expression between Protocorm-like Bodies and Leaves

“…DOPs have many pharmacological activities such as curving hypoglycemia [7], antioxidation [8], immunomodulation [9] and anti-tumor. Alkaloids of D. o cinale exhibit a variety of pharmacological activities such as antioxidant, anticancer, and neuroprotective activities [10], and avonoids of D. o cinale possess good functions on anti-cytotoxicity and antioxidant [11].…”