Bacteriophages as Factories for Eu₂O₃ Nanoparticle Synthesis

Abstract

The use of phage display to identify peptides with an ability to bind and synthesize EuO nanoparticles is demonstrated in this report. This is the first report of modified phages specifically binding a lanthanide. The peptides exposed on virions revealed very strong binding to EuO nanoparticles and the ability to catalyze EuO nanoparticles' formation from Eu(OH) and Eu(NO) solutions. The luminescence emission spectrum of Eu ions indicated that these ions existed mostly in sites deviated from the inversion symmetry in crystalline EuO aggregates and gelatinous Eu(OH) precipitate. The ability of phage-displayed peptides to catalyze formation of EuO nanoparticles provides a useful tool for a low-cost and effective synthesis of lanthanide nanoparticles, which serve as attractive biomedical sensors or fluorescent labels, among their other applications.

Phage-Based Applications in Synthetic Biology

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An effective antibiofilm strategy based on bacteriophages armed with silver nanoparticles

“…Previous studies have shown that genetically engineered M13 phages, exposing recombinant proteins, are capable of not only binding but also synthesizing both the desired peptides and metal nanoparticles on their surface 53 – 55 . These properties of modified M13 phages are intriguing, particularly because in all previous efforts the desired peptides were synthesized by standard chemical synthesis methods and then used for binding of metal nanoparticles.…”

Recent Progress of Rare Earth Oxides for Sensor, Detector, and Electronic Device Applications: A Review

“…64 Besides, several variants of bacteriophages have shown the capability of strong binding with catalyzation of Eu 2 O 3 nanoparticles, leading to nanoparticle synthesis or, in other words, an economic synthesis method for lanthanide nanoparticles suitable for biosensing applications such as fluorescent labels. 65 The synthesis of morphology-controlled CeO 2 -based glucose biosensor through electrodeposition has a tremendous influence on the surface structure and chemistry and the biosensing performance, as shown in Figure 5. 66 Carbon cloth/paper-based substrates directly influenced the surface nanostructures and resulted in enhanced performance.…”

“…The optoelectrical properties of sesquioxide-synthesized nanocomposites are, therefore, a remarkable addition in novel humidity sensors − for ambient humidity monitoring. The nanocrystalline structures and high photoluminescence properties have enhanced the use of R 2 O 3 -synthesized nanoparticles as an attractive material in novel biosensing applications of the medical sector. ,− Their unique traits have also been employed in a wide variety of industrial and consumer electronic device applications encompassing memory devices, ,− metal oxide semiconductors (MOSs), ,,, dielectric material devices, − highly efficient capacitors, − to energy storage devices. , The high dielectric constant, thermodynamic strength, high band energy gap, sputtering power, high power density, and longevity have immensely favored REOs as a suitable dopant for various electronic devices. ,, …”

“…In this regard, Er-doped Y 2 O 3 nanoparticles have shown sharp absorption and emission spectra in the near-infrared region . Besides, several variants of bacteriophages have shown the capability of strong binding with catalyzation of Eu 2 O 3 nanoparticles, leading to nanoparticle synthesis or, in other words, an economic synthesis method for lanthanide nanoparticles suitable for biosensing applications such as fluorescent labels . The synthesis of morphology-controlled CeO 2 -based glucose biosensor through electrodeposition has a tremendous influence on the surface structure and chemistry and the biosensing performance, as shown in Figure .…”