Effects of Noggin-Transfected Neural Stem Cells on Neural Functional Recovery and Underlying Mechanism in Rats with Cerebral Ischemia Reperfusion Injury

Zhu, Jun; Wang, Junjie; Ge, Guo; Kang, Chao-sheng

doi:10.1016/j.jstrokecerebrovasdis.2017.02.034

Cited by 17 publications

(9 citation statements)

References 37 publications

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“…As shown in Table 2, many studies have assessed NSC transplantation to treat ischemic brain injury in animal models. The initial hypotheses were based on the assumption that NSCs would replace lost neurons and restore connections in neuronal circuitry [94][95][96][97][98][99][100][101][102][103][104][105][106]. Indeed, analysis of graft-derived neuronal cells using immuno-electron microscopy and electrophysiological recording demonstrated increased connectivity, showing that NSCs could develop into functioning neurons to contribute to improved recovery from stroke and brain injury in rats [107,108].…”

Section: Experimental Studies Investigating Neural Stem Cells Transplmentioning

confidence: 99%

“…Although transplanted NSCs are expected to differentiate into various neural cells rather than other stem cells, it remains unclear to what extent this contributes to functional recovery. The current understanding is that transplanted NSCs likely prevent neuronal apoptosis, exert immunomodulatory effects inside and outside the brain, increase endogenous neuronal regeneration and angiogenesis, and inhibit glial scar formation mainly via paracrine and autocrine secretion of various neurotrophic factors rather than differentiation [ 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 109 , 110 ] ( Table 2 ).…”

Section: Neural Stem Cellsmentioning

confidence: 99%

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Recent Advances in Cell-Based Therapies for Ischemic Stroke

Suda

Nito

Yokobori

et al. 2020

IJMS

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Stroke is the most prevalent cardiovascular disease worldwide, and is still one of the leading causes of death and disability. Stem cell-based therapy is actively being investigated as a new potential treatment for certain neurological disorders, including stroke. Various types of cells, including bone marrow mononuclear cells, bone marrow mesenchymal stem cells, dental pulp stem cells, neural stem cells, inducible pluripotent stem cells, and genetically modified stem cells have been found to improve neurological outcomes in animal models of stroke, and there are some ongoing clinical trials assessing their efficacy in humans. In this review, we aim to summarize the recent advances in cell-based therapies to treat stroke.

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Section: Experimental Studies Investigating Neural Stem Cells Transplmentioning

confidence: 99%

Section: Neural Stem Cellsmentioning

confidence: 99%

Recent Advances in Cell-Based Therapies for Ischemic Stroke

Suda

Nito

Yokobori

et al. 2020

IJMS

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“…Noggin is a signaling molecule involved in embryonic development. Grafting NSCs modified by noggin gene reduced the percentage of apoptotic neurons and relieved neuronal morphological damage, which was accompanied by the decrease of the MDA levels, the SOD activity, and downregulation of the bone morphogenesis protein 4 (BMP4), VEGF, and bFGF proteins [133] .…”

Section: Relieve Neuronal Morphological Damagementioning

confidence: 99%

Pharmacological approaches promoting stem cell-based therapy following ischemic stroke insults

Zhu

Szeto²,

Bao

et al. 2018

Acta Pharmacol Sin

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Stroke can lead to long-term neurological deficits. Adult neurogenesis, the continuous generation of newborn neurons in distinct regions of the brain throughout life, has been considered as one of the appoaches to restore the neurological function following ischemic stroke. However, ischemia-induced spontaneous neurogenesis is not suffcient, thus cell-based therapy, including infusing exogenous stem cells or stimulating endogenous stem cells to help repair of injured brain, has been studied in numerous animal experiments and some pilot clinical trials. While the effects of cell-based therapy on neurological function during recovery remains unproven in randomized controlled trials, pharmacological agents have been administrated to assist the cell-based therapy. In this review, we summarized the limitations of ischemia-induced neurogenesis and stem-cell transplantation, as well as the potential proneuroregenerative effects of drugs that may enhance efficacy of cell-based therapies. Specifically, we discussed drugs that enhance proliferation, migration, differentiation, survival and function connectivity of newborn neurons, which may restore neurobehavioral function and improve outcomes in stroke patients.

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“…NSCs are multipotential stem cells with the capability to self-renew and can generate neurons, astrocytes, and oligodendrocytes [ 9 ]. NSCs play an important role both in basic research of neural development and wide potential for stem cell-based therapy in neurological diseases such as stroke, Parkinson's disease, and spinal cord injury [ 10 , 11 ]. It has been reported that an immortalized human NSC line, HB1.F3 (F3), was constructed from a 15-week gestational human fetal brain but this cell line is overexpresses v-myc oncogene with a retrovirus vector [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…NSCs play an important role both in basic research of neural development and wide potential for stem cell-based therapy in neurological diseases such as stroke, Parkinson's disease, and spinal cord injury [ 10 , 11 ]. It has been reported that an immortalized human NSC line, HB1.F3 (F3), was constructed from a 15-week gestational human fetal brain but this cell line is overexpresses v-myc oncogene with a retrovirus vector [ 10 ]. Previous studies show that human NSCs which transplant by intravenous injection can differentiate into diverse neural cell types and reduce the neurological damage after stroke [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

Han

Ren

et al. 2017

Stem Cells International

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Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.

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Effects of Noggin-Transfected Neural Stem Cells on Neural Functional Recovery and Underlying Mechanism in Rats with Cerebral Ischemia Reperfusion Injury

Cited by 17 publications

References 37 publications

Recent Advances in Cell-Based Therapies for Ischemic Stroke

Recent Advances in Cell-Based Therapies for Ischemic Stroke

Pharmacological approaches promoting stem cell-based therapy following ischemic stroke insults

Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

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