Isolation and phylogenetic analysis of three feline calicivirus strains from domestic cats in Jilin Province, China

et al. 2020

Preprint

Self Cite

Background: Feline calicivirus (FCV) is a common pathogen causing widely prevalent upper respiratory disease for kitten and felines in recent years. Due to the substantial genetic variability of the viral genes, existing vaccines cannot provide complete protection. Therefore, researches on FCV antiviral drugs have received much attention. Results: In this study, we found that copper chloride had dose-dependent antiviral effects against FCV in F81 cells. We also found that the combination of copper chloride and ribavirin had a synergistic effect against FCV in the F81 cells. In contrast, the combination of and horse anti-FCV immunoglobulin F(ab’)2 showed an antagonistic effect, likely because the copper chloride has an effect on the F(ab’)2 immunoglobulin; however, further research is needed to clarify this supposition.Conclusions: In summary, we found that copper chloride had low cytotoxicity and significant antiviral effects against FCV in F81 cells, providing a new drug candidate for the prevention and treatment of FCV infection.

Section: Antiviral Effect Of Copper Chloride On Different Strainsmentioning

confidence: 85%

“…The FCV CH-SH strains and F81 cells were provided by the Institute of Military Veterinary Medicine, Academy of Military Medical Science. FCV CH-JL1/CH-JL2/CH-JL3/CH-JL4 were isolated and stored in our laboratory [20]. The horse anti-FCV immunoglobulin F(ab') 2 was produced and stored in our laboratory [30].…”

Section: Viruses Cells and Compoundsmentioning

confidence: 99%

Antiviral effect of copper chloride on feline calicivirus and Synergy with Ribavirin in vitro

et al. 2020

Preprint

Self Cite

“…Feline kidney fibroblast-like monolayer cells (F81) and the CH-SH strain of FCV were provided by The Institute of Military Veterinary Medicine (Changchun, China). CH-JL1, CH-JL2, CH-JL3 and CH-JL4 strains of FCV were isolated and stored in our laboratory (Wang et al, 2017;Zhao et al, 2017). MZR (#M129842) and NTZ (#N159057) were purchased from Aladdin, China.…”

Section: Cells Virus Strains and Compoundsmentioning

confidence: 99%

Nitazoxanide protects cats from feline calicivirus infection and acts synergistically with mizoribine in vitro

Xie

et al. 2020

Antiviral Research

Self Cite

Feline calicivirus (FCV) is a highly contagious pathogen that causes acute upper respiratory infections and oral disease in cats, thus seriously endangering feline health. Recently, there have been outbreaks of particularly virulent variant strains of FCV, which can cause both acute symptoms and fatal systemic disease. The discovery of effective antiviral agents to treat FCV infection is, therefore, gradually assuming increased importance. In this study, we showed that both nitazoxanide and mizoribine had antiviral activity in F81 cells infected with different strains of FCV and also demonstrated a synergistic effect between the two drugs. Experiments in cats challenged with FCV showed that nitazoxanide significantly reduced the clinical symptoms of FCV infection, reduced viral load in the trachea and lungs, and reduced viral shedding. Our results showed that nitazoxanide and mizoribine could potentially be used as therapeutic agents to treat FCV infection.

“…The FCV CH-SH strains and F81 cells were provided by the Institute of Military Veterinary Medicine, Academy of Military Medical Science. FCV CH-JL1/CH-JL2/CH-JL3/CH-JL4 were isolated and stored in our laboratory [38]. The horse anti-FCV immunoglobulin F(ab') 2 was produced and stored in our laboratory [39].…”

Section: Viruses Cells and Compoundsmentioning

confidence: 99%

Antiviral effect of copper chloride on feline calicivirus and Synergy with Ribavirin in vitro

et al. 2020

Preprint

Self Cite

Background: Feline calicivirus (FCV) is a common pathogen causing widely prevalent upper respiratory disease for kitten and felines in recent years. Due to the substantial genetic variability of the viral genes, existing vaccines cannot provide complete protection. Therefore, researches on FCV antiviral drugs have received much attention. Results: In this study, we found that copper chloride had dose-dependent antiviral effects against FCV in F81 cells. We also found that the combination of copper chloride and ribavirin had a synergistic effect against FCV in the F81 cells. In contrast, the combination of and horse anti-FCV immunoglobulin F(ab’) 2 showed an antagonistic effect, likely because the copper chloride has an effect on the F(ab’) 2 immunoglobulin; however, further research is needed to clarify this supposition. Conclusions: In summary, we found that copper chloride had low cytotoxicity and significant antiviral effects against FCV in F81 cells, providing a new drug candidate for the prevention and treatment of FCV infection.