The Impact of Chronic Sleep Restriction on Neuronal Number and Volumetric Correlates of the Dorsal Respiratory Nuclei in a Rat Model

Kamali, Ali-Mohammad; Noorafshan, Ali; Karimi, Fatemeh; Karbalay‐Doust, Saied; Nami, Mohammad

doi:10.1093/sleep/zsx072

Cited by 15 publications

(14 citation statements)

References 30 publications

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“…In this study, the number of ganglionic cells in the CSD decreased in comparison to the control group, which is almost in line with the previous studies that showed a decrease in the number of neurons in different parts of the brain, such as the hippocampus, dentate gyrus [52], and dorsal respiratory nuclei in the brainstem following the CSD [22].…”

Section: Discussionsupporting

confidence: 92%

“…In this study, the MMPM was designed to reduce individual stress, which included a recovery time window and provide social communication amongst animals without hindering free movement [22]. The tanks were filled with water to a level one cm below the surface of the platforms or the grid.…”

Section: Induction Of Chronic Sleep Deprivationmentioning

confidence: 99%

“…Previous studies have shown that sleep deprivation can lead to structural changes and apoptosis and loss of neurons in different parts of the brain such as the prefrontal cortex, hippocampus [9], and respiratory nuclei of the brain stem [22]. A recent report on humans showed that gray matter volume is reduced upon sleep disturbance [23].…”

Section: Introductionmentioning

confidence: 99%

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Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

et al. 2020

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Background: In modern societies, sleep deprivation is a serious health problem. This problem could be induced by a variety of reasons, including lifestyle habits or neurological disorders. Chronic sleep deprivation (CSD) could have complex biological consequences, such as changes in neural autonomic control, increased oxidative stress, and inflammatory responses. The superior cervical ganglion (SCG) is an important sympathetic component of the autonomic nervous system. CSD can lead to a wide range of neurological consequences in SCG, which mainly supply innervations to circadian system and other structures. As the active component of Curcuma longa, curcumin possesses many therapeutic properties; including neuroprotective. This study aimed to evaluate the effect of CSD on the SCG histomorphometrical changes and the protective effect of curcumin in preventing these changes. Methods: Thirty-six male rats were randomly assigned to the control, curcumin, CSD, CSD + curcumin, grid floor control, and grid floor + curcumin groups. The CSD was induced by a modified multiple platform apparatus for 21 days and animals were sacrificed at the end of CSD or treatment, and their SCGs removed for stereological and TUNEL evaluations and also spatial arrangement of neurons in this structure. Results: Concerning stereological findings, CSD significantly reduced the volume of SCG and its total number of neurons and satellite glial cells in comparison with the control animals (P < 0.05). Treatment of CSD with curcumin prevented these decreases. Furthermore, TUNEL evaluation showed significant apoptosis in the SCG cells in the CSD group, and treatment with curcumin significantly decreased this apoptosis (P < 0.01). This decrease in apoptosis was observed in all control groups that received curcumin. CSD also changed the spatial arrangement of ganglionic neurons into a random pattern, whereas treatment with curcumin preserved its regular pattern. Conclusions: CSD could potentially induce neuronal loss and structural changes including random spatial distribution in the SCG neurons. Deleterious effects of sleep deprivation could be prevented by the oral administration of curcumin. Furthermore, the consumption of curcumin in a healthy person might lead to a reduction of cell death.

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Section: Discussionsupporting

confidence: 92%

Section: Induction Of Chronic Sleep Deprivationmentioning

confidence: 99%

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Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

et al. 2020

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“…The CSR process continued for 21 days during which the laboratory animals were kept in the multiple-platform apparatus for 18 h (from 06:00 PM to 12:00 PM the next day), followed by a 6-h sleep recovery window (12:00 PM to 18:00). This time interval (06:00 pm to 12:00 pm the next day) was selected because, in this period, rats are known to attain the highest paradoxical (REM) sleep whereas the homeostatic pressure of non-REM sleep has been already yielded [21,22]. The environmental control group was positioned in the grid-floor box, in which animals were placed onto a stainless steel grid.…”

Section: Chronic Sleep Restrictionmentioning

confidence: 99%

“…The rods were fixed 2.3 cm apart letting the animals lie down while preventing them from falling in the water. The tails could have been immersed into the water [22,23].…”

Section: Chronic Sleep Restrictionmentioning

confidence: 99%

Motor Control in Chronically Sleep-Restricted Rats: A Behavioral Approach

et al. 2018

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Objective To consider the effect of chronic sleep restriction (CSR) on motor impairment in sleep-restricted rats. Methods A total of 21 adult male Sprague-Dawley rats were assigned to three groups including control, grid-floor control and CSR using the modified multiple-platform method. At the end of experiment, motor performance was assessed by means of the rotarod test. Results Performance of the CSR rats in the fixed and accelerating speed rotarod tasks was impaired and their endurance time was less than the control group (p < 0.01). Conclusion The rotarod motor performance of the CSR rats was reduced, suggesting an impaired motor control function following sleep restriction.

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Mapping of the excitatory, inhibitory, and modulatory afferent projections to the anatomically defined active expiratory oscillator in adult male rats

Biancardi

Saini

Pageni

et al. 2020

J of Comparative Neurology

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The lateral parafacial region (pF L ; which encompasses the parafacial respiratory group, pFRG) is a conditional oscillator that drives active expiration during periods of high respiratory demand, and increases ventilation through the recruitment of expiratory muscles. The pF L activity is highly modulated, and systematic analysis of its afferent projections is required to understand its connectivity and modulatory control. We combined a viral retrograde tracing approach to map direct brainstem projections to the putative location of pF L , with RNAScope and immunofluorescence to identify the neurochemical phenotype of the projecting neurons. Within the medulla, retrogradely-labeled, glutamatergic, glycinergic and GABAergic neurons were found in the ventral respiratory column (Bötzinger and preBötzinger Complex [preBötC], ventral respiratory group, ventral parafacial region [pF V ] and pF L), nucleus of the solitary tract (NTS), reticular formation (RF), pontine and midbrain vestibular nuclei, and medullary raphe. In the pons and midbrain, retrogradely-labeled neurons of the same phenotypes were found in the Kölliker-Fuse and parabrachial nuclei, periaqueductal gray, pedunculopontine nucleus (PPT) and laterodorsal tegmentum (LDT). We also identified somatostatin-expressing neurons in the preBötC and PHOX2B immunopositive cells in the pF V , NTS, and part of the RF. Surprisingly, we found no catecholaminergic neurons in the NTS, A5 or Locus Coeruleus, no serotoninergic raphe neurons nor any cholinergic neurons in the PPT and LDT that projected to the pF L. Our results indicate that pF L neurons receive extensive excitatory and inhibitory inputs from several respiratory and nonrespiratory related brainstem regions that could contribute to the complex modulation of the conditional pF L oscillator for active expiration.

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The Impact of Chronic Sleep Restriction on Neuronal Number and Volumetric Correlates of the Dorsal Respiratory Nuclei in a Rat Model

Abstract: CSR may potentially induce neuronal loss and structural changes in the dorsal respiratory nuclei.

Cited by 15 publications

References 30 publications

Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

Motor Control in Chronically Sleep-Restricted Rats: A Behavioral Approach

Mapping of the excitatory, inhibitory, and modulatory afferent projections to the anatomically defined active expiratory oscillator in adult male rats

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