Characterization of male germ cell markers in canine testis

Lee, Won Y.; Lee, Ran; Park, Hyun-Jung; Tae, Jeong; Park, Chankyu; Kim, Jin‐Hoi; Jhun, Hyunjhung; Lee, Ji-Heon; Hur, Tai-Young; Song, Hyuk

doi:10.1016/j.anireprosci.2017.01.002

Cited by 17 publications

(16 citation statements)

References 27 publications

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“…We investigated the histological and cellular changes in dog testes following busulfan treatment to establish sterile males to serve as germ cell transplant recipients. Our previous study reported that PGP9.5 was detected in undifferentiated spermatogonia, SCP3 was detected in primary spermatocytes, and acrosin was detected in the spermatids and spermatozoa in dog testes [ 11 ]. We therefore also analyzed the expression of these markers in dog testes after busulfan treatment.…”

Section: Discussionmentioning

confidence: 99%

Dose-dependent effects of busulfan on dog testes in preparation for spermatogonial stem cell transplantation

et al. 2017

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Successful male germ cell transplantation requires depletion of the host germ cells to allow efficient colonization of the donor spermatogonial stem cells. Although a sterilizing drug, busulfan, is commonly used for the preparation of recipient models before transplantation, the optimal dose of this drug has not yet been defined in dogs. In this study, 1-year-old mongrel dogs were intravenously injected with three different concentrations of busulfan (10, 15, or 17.5 mg/kg). Four weeks after busulfan treatment, no fully matured spermatozoa were detected in any of the busulfan-treated groups. However, small numbers of PGP9.5-positive spermatogonia were detected in all treatment groups, although no synaptonemal complex protein-3-positive spermatocytes were detected. Of note, acrosin-positive spermatids were not detected in the dogs treated with 15 or 17.5 mg/kg busulfan, but were detected in the other group. Eight weeks after busulfan treatment, the dogs treated with 10 mg/kg busulfan fully recovered, but those in the other groups did not. PGP9.5-positive spermatogonia were detected in the 10 mg/kg group, and at a similar level as in the control group, but these cells were rarely detected in the 15 and 17.5 mg/kg groups. These results suggest that a dose of 15-17.5 mg/kg is optimal for ablative treatment with busulfan to prepare the recipient dogs for male germ cell transplantation. At least eight weeks should be allowed for recovery. The results of this study might facilitate the production of recipient dogs for male germ cell transplantation and can also contribute to studies on chemotherapy.

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Section: Discussionmentioning

confidence: 99%

Dose-dependent effects of busulfan on dog testes in preparation for spermatogonial stem cell transplantation

et al. 2017

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“…The testicular tissue fragments of the experimental groups, in addition to the tracing of DiI, were subjected to immunohistochemistry after tissue processing. To confirm the nature of SSCs, spermatocyte and spermatozoa the PLZF protein [17], SCP3 protein [20] and the ACRBP protein [21] were detected, respectively. The procedure of immunocytochemistry was performed according to previous study [22].…”

Section: Methodsmentioning

confidence: 99%

In vitro transplantation of spermatogonial stem cells isolated from human frozen–thawed testis tissue can induce spermatogenesis under 3-dimensional tissue culture conditions

Mohaqiq

Movahedin

Mazaheri³

et al. 2019

Biol Res

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Background Sperm production is one of the most complex biological processes in the body. In vitro production of sperm is one of the most important goals of researches in the field of male infertility treatment, which is very important in male cancer patients treated with gonadotoxic methods and drugs. In this study, we examine the progression of spermatogenesis after transplantation of spermatogonial stem cells under conditions of testicular tissue culture. Results Testicular tissue samples from azoospermic patients were obtained and then these were freeze–thawed. Spermatogonial stem cells were isolated by two enzymatic digestion steps and the identification of these cells was confirmed by detecting the PLZF protein. These cells, after being labeled with DiI, were transplanted in azoospermia adult mice model. The host testes were placed on agarose gel as tissue culture system. After 8 weeks, histomorphometric, immunohistochemical and molecular studies were performed. The results of histomorphometric studies showed that the mean number of spermatogonial cells, spermatocytes and spermatids in the experimental group was significantly more than the control group (without transplantation) (P < 0.05) and most of the cells responded positively to the detection of DiI. Immunohistochemical studies in host testes fragments in the experimental group express the PLZF , SCP3 and ACRBP proteins in spermatogonial cells, spermatocyte and spermatozoa, respectively, which confirmed the human nature of these cells. Also, in molecular studies of PLZF , Tekt1 and TP1 , the results indicated that the genes were positive in the test group, while not in the control group. Conclusion These results suggest that the slow freezing of SSCs can support the induction of spermatogenesis to produce haploid cells under the 3-dimensional testicular tissue culture.

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“…In a Beagle dog, the number of spermatogonia and Sertoli cells increases in 2-3 months old puppies. It is possible to find single and paired spermatogonia, but aligned spermatogonia were only observed in 3 months old [31] In general, dogs reach puberty, between 6 and 24 months of life, however, this variation depends on the gender, breed, hormone levels, food availability and weight [31][32][33].…”

Section: Spermatogonial Canine Stem Cellsmentioning

confidence: 99%

“…During this period, physiological and hormonal changes begin, such as the production of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which exert their actions on spermatogenesis mainly through the regulation of Sertoli cell factors and regulation of sperm production [35]. According to the literature, in canine, the spermatogenesis process is initiated at 7 months of age; however, at 10-12 months of age, it is possible to verify the presence of sperm in canine ejaculate [32]. However, sexual maturity in canines occurs when spermatogenic yield reaches maximum levels (generally, 3-6 years of age).…”

Section: Spermatogonial Canine Stem Cellsmentioning

confidence: 99%

Step by Step about Germ Cells Development in Canine

Souza

Pieri

Martins

2021

Animals

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Primordial germ cells (PGCs) have been described as precursors of gametes and provide a connection within generations, passing on the genome to the next generation. Failures in the formation of gametes/germ cells can compromise the maintenance and conservation of species. Most of the studies with PGCs have been carried out in mice, but this species is not always the best study model when transposing this knowledge to humans. Domestic animals, such as canines (canine), have become a valuable translational research model for stem cells and therapy. Furthermore, the study of canine germ cells opens new avenues for veterinary reproduction. In this review, the objective is to provide a comprehensive overview of the current knowledge on canine germ cells. The aspects of canine development and germ cells have been discussed since the origin, specifications, and development of spermatogonial canine were first discussed. Additionally, we discussed and explored some in vitro aspects of canine reproduction with germ cells, such as embryonic germ cells and spermatogonial stem cells.

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Characterization of male germ cell markers in canine testis

Cited by 17 publications

References 27 publications

Dose-dependent effects of busulfan on dog testes in preparation for spermatogonial stem cell transplantation

Dose-dependent effects of busulfan on dog testes in preparation for spermatogonial stem cell transplantation

In vitro transplantation of spermatogonial stem cells isolated from human frozen–thawed testis tissue can induce spermatogenesis under 3-dimensional tissue culture conditions

Step by Step about Germ Cells Development in Canine

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