Tropism and virulence of Cutibacterium (formerly Propionibacterium ) acnes involved in implant-associated infection

Aubin, Guillaume Ghislain; Lavigne, Jean‐Philippe; Foucher, Yohann; Dellière, Sarah; Lepelletier, Didier; Corvec, Stéphane

doi:10.1016/j.anaerobe.2017.04.009

Cited by 39 publications

(23 citation statements)

References 34 publications

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“…This bacterium has been described both as a commensal of the human skin playing a role in the balance of normal skin microbiota and as a pathogen bacterium . For instance, C. acnes implication was previously reported in cutaneous dermatoses such as acne and folliculitis, but also in other types of affections including deep medical device infections, prostate cancer and sarcoidosis . Cutibacterium acnes strains have long been classified into several subtypes, currently, it is subdivided into six main phylotypes: IA1, IA2, IB, IC, II and III …”

Section: Introductionmentioning

confidence: 99%

Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Dagnelie

Corvec²,

Saint‐Jean

et al. 2019

Acad Dermatol Venereol

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Background Acne has long been understood as a multifactorial chronic inflammatory disease of the pilosebaceous follicle, where Cutibacterium acnes (subdivided into six main phylotypes) is a crucial factor. In parallel, the loss of microbial diversity among the skin commensal communities has recently been shown as often accompanied by inflammatory skin disorders. Objective This study investigated the association of C. acnes phylotype diversity loss and the impact on Innate Immune System (IIS) activation. Methods The IIS response of skin after incubation with phylotypes IA1, II or III individually and with the combination of IA1 + II + III phylotypes, was studied in an in vitro skin explant system. The inflammatory response was monitored by immunohistochemistry and ELISA assays, targeting a selection of Innate Immune Markers (IIMs) (IL‐6, IL‐8, IL‐10, IL‐17, TGF‐β). Results IIMs were significantly upregulated in skin when being incubated with phylotype IA1 alone compared with the combination IA1 + II + III. In parallel, ELISA assays confirmed these results in supernatants for IL‐17, IL‐8 and IL‐10. Conclusion We identify the loss of C. acnes phylotype diversity as a trigger for IIS activation, leading to cutaneous inflammation. These innovative data underline the possibility to set up new approaches to treat acne. Indeed, maintaining the balance between the different phylotypes of C. acnes may be an interesting target for the development of drugs.

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Section: Introductionmentioning

confidence: 99%

Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Dagnelie

Corvec²,

Saint‐Jean

et al. 2019

Acad Dermatol Venereol

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“…Most individuals have two or more different subtypes present on their skin simultaneously, and the distribution of various subtypes differs widely between subjects (13). Some subtypes are associated with pathogenic states rather than commensal states (13)(14)(15). Certain subtypes may be better adapted at thriving in the deep tissues and causing clinical infection than others (13,14,16).…”

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confidence: 99%

“…Some subtypes are associated with pathogenic states rather than commensal states (13)(14)(15). Certain subtypes may be better adapted at thriving in the deep tissues and causing clinical infection than others (13,14,16). Also, some subtypes may be more strongly associated with clinical markers of infection, such as hemolysis, and antibiotic resistance (16)(17)(18)(19)(20)(21)(22).…”

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confidence: 99%

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Cutibacterium acnes Isolates from Deep Tissue Specimens Retrieved during Revision Shoulder Arthroplasty: Similar Colony Morphology Does Not Indicate Clonality

2020

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Cutibacterium acnes is the most common bacterium associated with periprosthetic shoulder infections. Sequencing of C. acnes has been proposed as a potential rapid diagnostic tool and a method of determining subtypes associated with pathogenicity and antibiotic resistance patterns. When multiple deep samples from the same surgery are culture positive for the same species and the isolates show the same culture phenotype, it is typically assumed that these isolates are clonal. However, it is well-known that C. acnes is not clonal on the skin of most individuals. We hypothesized that the C. acnes bacteria recovered at the time of revision shoulder arthroplasty would often represent more than one subtype, and we tested this hypothesis in this work. For patients undergoing revision shoulder arthroplasty, multiple samples from the surgical field were taken. For those patients with multiple samples that were culture positive for C. acnes, isolates from each sample were subjected to full genome sequencing. Of 11 patients, 5 (45%) had different subtypes of C. acnes within the deep tissues even though the colony morphology was similar. One patient had four subtypes in the deep tissues, while four patients had two different subtypes. Up to four different subtypes of C. acnes were observed in the deep tissues of a single patient. Clonality of C. acnes isolates from deep specimens from a potential periprosthetic shoulder infection cannot be assumed. Sequence-based characterization of virulence and antibiotic resistance may require testing of multiple deep specimens.

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“…acnes type IB is not as abundant as type IA on the skin, it is still a common skin bacterium. However, the abundance of type IA on the skin may explain why the immune system is more adapt in responding to those isolates through aggregation, constantly being stimulated by these strains [ 10 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes

Petersson¹,

Kilsgård²,

Shannon³

et al. 2018

PLoS ONE

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Cutibacterium (Propionibacterium) acnes, considered a part of the skin microbiota, is one of the most commonly isolated anaerobic bacteria from medical implants in contact with plasma. However, the precise interaction of C. acnes with blood cells and plasma proteins has not been fully elucidated. Herein, we have investigated the molecular interaction of C. acnes with platelets and plasma proteins. We report that the ability of C. acnes to aggregate platelets is dependent on phylotype, with a significantly lower ability amongst type IB isolates, and the interaction of specific donor-dependent plasma proteins (or concentrations thereof) with C. acnes. Pretreatment of C. acnes with plasma reduces the lag time before aggregation demonstrating that pre-deposition of plasma proteins on C. acnes is an important step in platelet aggregation. Using mass spectrometry we identified several plasma proteins deposited on C. acnes, including IgG, fibrinogen and complement factors. Inhibition of IgG, fibrinogen or complement decreased C. acnes-mediated platelet aggregation, demonstrating the importance of these plasma proteins for aggregation. The interaction of C. acnes and platelets was visualized using fluorescence microscopy, verifying the presence of IgG and fibrinogen as components of the aggregates, and co-localization of C. acnes and platelets in the aggregates. Here, we have demonstrated the ability of C. acnes to activate and aggregate platelets in a bacterium and donor-specific fashion, as well as added mechanistic insights into this interaction.

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Tropism and virulence of Cutibacterium (formerly Propionibacterium ) acnes involved in implant-associated infection

Cited by 39 publications

References 34 publications

Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Cutibacterium acnes Isolates from Deep Tissue Specimens Retrieved during Revision Shoulder Arthroplasty: Similar Colony Morphology Does Not Indicate Clonality

Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes

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