Complete Genome Sequence of
            <i>Tessaracoccus</i>
            sp. Strain T2.5-30 Isolated from 139.5 Meters Deep on the Subsurface of the Iberian Pyritic Belt

Leandro, Tânia; Costa, Milton S. da; Sanz, J. L.; Amils, Ricardo

doi:10.1128/genomea.00238-17

Cited by 4 publications

(6 citation statements)

References 17 publications

(20 reference statements)

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“…Recently the complete genome of strain Tessaracoccus sp. strain T2.5-30 isolated in this study has been sequenced ( Leandro et al., 2017 ), as well as a draft genome of strain T2.26MG-112.2 have also been obtained. Analysis and comparison with the genomes of other members of these genera should shed light on their role in the IPB subsurface.…”

Section: Discussionmentioning

confidence: 99%

Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt

Leandro

Rodríguez

Rojas

et al. 2018

Heliyon

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Two deep boreholes were drilled at 320 and 620 meters below surface in the Iberian Pyritic Belt (IPB) at Peña de Hierro (Huelva, Southwestern Spain). Cores were sampled and used for the establishment of enrichment cultures with methanogenic activity. The cultivable diversity of these enrichments was accessed using different cultivation techniques and several isolates were recovered in pure culture from various depths in both boreholes. Although no archaeal isolates were obtained in pure culture, strict anaerobes and facultative anaerobic bacteria belonging to the phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were isolated and identified using the 16S rRNA gene sequence.Analysis of three selected enrichment cultures by amplification of both bacterial and archaeal 16S rRNA gene followed by pyrosequencing revealed further information on the populations enriched. The archaeal sequences obtained from the methanogenic enrichment cultures belonged to the orders Methanosarcinales and Methanocellales. To best of our knowledge this is the first report of enrichment in members of the Methanocellales in a deep terrestrial subsurface ecosystem. Several bacterial populations, predominantly consisting of Firmicutes and Proteobacteria, were also enriched. The prevalent microbial populations enriched as detected by pyrosequencing analysis, as well as the bacterial isolates cultivated were affiliated with known fermentative, sulfate reducing and acetogenic bacteria or methanogenic archaea. Our results show a great diversity in the microbial communities of the IPB deep subsurface.

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Section: Discussionmentioning

confidence: 99%

Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt

Leandro

Rodríguez

Rojas

et al. 2018

Heliyon

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“…In addition, we used specific probes to detect the presence of microorganisms that have been isolated or identified in the subsurface of the IPB (Leandro et al ., 2018) and for which sequenced genomes are available (Leandro et al ., 2017; García et al ., 2018; Mariñán et al ., 2019; Rodríguez‐Robles et al ., 2019; de Polanco et al ., 2020; Martínez et al ., 2021). This allowed us to identify possible metabolisms that could generate the detected gases.…”

Section: Resultsmentioning

confidence: 99%

Biological production of H₂, CH₄ and CO₂ in the deep subsurface of the Iberian Pyrite Belt

Sanz

Rodríguez

Escudero

et al. 2021

Environmental Microbiology

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Most of the terrestrial deep subsurfaces are oligotrophic environments in which some gases, mainly H 2 , CH 4 and CO 2 , play an important role as energy and/or carbon sources. In this work, we assessed their biotic and abiotic origin in samples from subsurface hard-rock cores of the Iberian Pyrite Belt (IPB) at three different depths (414, 497 and 520 m). One set of samples was sterilized (abiotic control) and all samples were incubated under anaerobic conditions. Our results showed that H 2 , CH 4 and CO 2 remained low and constant in the sterilized controls while their levels were 4, 4.1 and 2.5 times higher respectively, in the unsterilized samples compared to the abiotic controls. The δ 13 C CH4 -values measured in the samples (range À31.2 to À43.0 ‰) reveals carbon isotopic signatures that are within the range for biological methane production. Possible microorganisms responsible for the biotic production of the gases were assessed by CARD-FISH. The analysis of sequenced genomes of detected microorganisms within the subsurface of the IPB allowed to identify possible metabolic activities involved in H 2 (Rhodoplanes, Shewanella and Desulfosporosinus), CH 4 (Methanobacteriales) and CO 2 production. The obtained results suggest that part of the H 2 , CH 4 and CO 2 detected in the deep subsurface has a biological origin.

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“…This activity could also be visualized through fluorescence microscopy of native subsurface samples by the co‐occurrence of ferric iron, the oxidation product, and the presence of nitrate‐reducing microorganisms, Acidovorax and Tessaracoccus , clearly identified by CARD‐FISH (Figure S8 ). The genomic analysis of these microorganisms showed the lack of recognizable genes associated with this metabolic activity (Carlson et al, 2013 ; Leandro et al, 2017 ; Martínez et al, 2020 ; Mateos et al, 2022 ; Puente‐Sánchez, Pieper, & Arce‐Rodríguez, 2016 ), so we have to conclude that the observed oxidation of iron, at least for the tested microorganisms, is due to the chemical oxidation of Fe promoted by the high oxidizing capacity of nitrite and nitric oxide generated by the use of nitrate as electron acceptor. These results strongly support the notion that some nitrate reducers can efficiently generate reactive nitrogen species able to oxidize iron under strict anaerobic conditions (Bryce et al, 2018 ; Carlson et al, 2013 ; Straub et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

“…The sequenced genomes of Tessaracoccus lapidicaptus IPBSL‐7 (Puente‐Sánchez, Pieper, & Arce‐Rodríguez, 2016 ), Tessaracoccus sp. T2.5‐30 (Leandro et al, 2017 ), Brevundimonas sp. T2.26MG‐97 (Rodríguez‐Robles et al, 2019 ), Rhizobium sp.…”

Section: Methodsmentioning

confidence: 99%

Coupled C, H, N, S and Fe biogeochemical cycles operating in the continental deep subsurface of the Iberian Pyrite Belt

Amils

Escudero

Oggerin

et al. 2022

Environmental Microbiology

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Microbial activity is a major contributor to the biogeochemical cycles that make up the life support system of planet Earth. A 613 m deep geomicrobiological perforation and a systematic multi‐analytical characterization revealed an unexpected diversity associated with the rock matrix microbiome that operates in the subsurface of the Iberian Pyrite Belt (IPB). Members of 1 class and 16 genera were deemed the most representative microorganisms of the IPB deep subsurface and selected for a deeper analysis. The use of fluorescence in situ hybridization allowed not only the identification of microorganisms but also the detection of novel activities in the subsurface such as anaerobic ammonium oxidation (ANAMMOX) and anaerobic methane oxidation, the co‐occurrence of microorganisms able to maintain complementary metabolic activities and the existence of biofilms. The use of enrichment cultures sensed the presence of five different complementary metabolic activities along the length of the borehole and isolated 29 bacterial species. Genomic analysis of nine isolates identified the genes involved in the complete operation of the light‐independent coupled C, H, N, S and Fe biogeochemical cycles. This study revealed the importance of nitrate reduction microorganisms in the oxidation of iron in the anoxic conditions existing in the subsurface of the IPB.

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Complete Genome Sequence of Tessaracoccus sp. Strain T2.5-30 Isolated from 139.5 Meters Deep on the Subsurface of the Iberian Pyritic Belt

Cited by 4 publications

References 17 publications

Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt

Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt

Biological production of H₂, CH₄ and CO₂ in the deep subsurface of the Iberian Pyrite Belt

Coupled C, H, N, S and Fe biogeochemical cycles operating in the continental deep subsurface of the Iberian Pyrite Belt

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Complete Genome Sequence of Tessaracoccus sp. Strain T2.5-30 Isolated from 139.5 Meters Deep on the Subsurface of the Iberian Pyritic Belt

Cited by 4 publications

References 17 publications

Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt

Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt

Biological production of H2, CH4 and CO2 in the deep subsurface of the Iberian Pyrite Belt

Coupled C, H, N, S and Fe biogeochemical cycles operating in the continental deep subsurface of the Iberian Pyrite Belt

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Biological production of H₂, CH₄ and CO₂ in the deep subsurface of the Iberian Pyrite Belt