Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers

Banjanac, Tijana; Dragićević, Milan; Šiler, Branislav; Gašić, Uroš; Bohanec, B.; Živković, Jelena; Trifunović, Snežana; Mišić, Danijela

doi:10.1016/j.phytochem.2017.04.005

Cited by 42 publications

(42 citation statements)

References 54 publications

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“…Surface-sterilized seeds were germinated on hormone-free half strength MS (½ MS) medium (Murashige and Skoog, 1962) solidified with 0.7% agar, supplemented with 3 % sucrose and 100 mg L -1 myo-inositol and adjusted to pH 5.8. Ten genotypes of 2x and 4x centaury plants, with ploidy levels confirmed earlier by flow cytometry as described by Šiler (2012) and Banjanac et al (2017), were randomly selected from in vitro pool of seedlings maintained on ½ MS. For the induction of in vitro morphogenesis, root explants, 1.0-1.5 cm in length, were excised either from basal, middle or apical root zone of 3 month-old plants and cultured on ½ MS medium separately, under a 16/8 h light/dark photoperiod. Basal segments were sections in the root zone nearest to the plantlet base, while apical sections contained the root apex.…”

Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

“…Quantification of iridoid glycosides (loganic acid, loganin), secoiridoid glycosides (secologanin, sweroside, swertiamarin, gentiopicrin) and xantones (decussatin, eustomin, methylbellidifolin, desmethyleustomin) in methanol extracts of C. erythraea was performed using UHPLC/DAD/MS 2 analysis as previously described by Banjanac et al (2017). Instrumentation consisted of Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific, Bremen, Germany) with DAD and a triple quadrupole mass spectrometer (TSQ Quantum access max, Thermo Fisher Scientific, Basel, Switzerland).…”

Section: Uhplc/dad/ms 2 Quantitative Analysis Of Targeted Metabolitesmentioning

confidence: 99%

“…Samples were chromatographically separated on Hypersil gold C18 column (50 × 2.1 mm) with 1.9 µm particle size (Thermo Fish-erScientific, USA), thermostated at 40°C. Mobile phase, flow rate and gradient elution were adjusted according to Banjanac et al (2017), as well as heated electrospray ionization (HESI) source of the mass spectrometer. All solvents were of LC-MS grade (Fisher Scientific UK, Leics, UK), and ultra-pure deionized water was generated using Water Purification System (New Human Power I Integrate, Human Corporation, Republic of Korea).…”

Section: Uhplc/dad/ms 2 Quantitative Analysis Of Targeted Metabolitesmentioning

confidence: 99%

“…The production of the synthetic polyploids is convenient because they can be compared to the diploid counterparts; however, tetraploid genotypes with natural origin can display a unique genome composition, since they have existed in natural environment for maybe hundreds of generations shaping their genetic background (Orzechowska et al, 2016). C. erythraea is commonly found in tetraploid forms in nature (Banjanac et al, 2017;Šiler, 2012). Tetraploid populations are mainly distributed in central and northern Europe while diploid populations occurred in Mediterranean region (Mansion et al, 2005;van der Sluis, 1985).…”

Section: Introductionmentioning

confidence: 99%

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Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Filipović¹,

Šiler²,

Nestorović-Živković³

et al. 2019

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The impact of ploidy level on both the regenerative potential under in vitro conditions and the production of major bioactive specialized metabolites, such as iridoids and xanthones, was examined in Centaurium erythraea Rafn. Shoot regeneration frequency was genotype dependent, but not affected by explant ploidy level. In most cases, the regenerated shoots of autotetraploid (4x) genotypes were more robust than diploid (2x) ones. Regeneration efficiency of root explants declined from the apical to the basal root segment. Shoot and root biomass production of two month-old plants was not significantly different between 2x and 4x genotypes. Both 4x and 2x genotypes were characterized by the predominance of secoiridoid glucoside gentiopicrin in shoots and roots, which is followed by sweroside and swertiamarin. Loganic acid, loganin and secologanin were much less abundant. Methylbellidifolin was the major xanthone in both shoots and roots. Diploid plants showed higher biosynthetic capacity for the production of secoiridoids and xanthones in both shoots and roots. Results highlight a higher potential of diploid C. erythraea genotypes for biotechnology-based sustainable production of secoiridoids in comparison to tetraploid genotypes.

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Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

Section: Uhplc/dad/ms 2 Quantitative Analysis Of Targeted Metabolitesmentioning

confidence: 99%

Section: Uhplc/dad/ms 2 Quantitative Analysis Of Targeted Metabolitesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Filipović¹,

Šiler²,

Nestorović-Živković³

et al. 2019

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“…Further, phenolics are well-known as potential tool for chemotaxonomic characterization for different plant species [21][22][23][24][25][26] or materials such as pollen [27] and honey. [28] Knowing that xanthone, isoflavone and flavonoid derivatives are almost exclusively present in Iridaceae family plants [29] and antioxidant properties of polyphenols, the aim of this work was to characterize the phytochemical composition and antioxidant properties of rhizomes, green parts and flowers of three mentioned Iris species.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above‐Ground Vegetative Parts and Flower of Three Iris Species

Kostić

Gašić

Pešić

et al. 2019

Chemistry & Biodiversity

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This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above‐ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC‐Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C‐glycosides, luteolin O‐glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.

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Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

Aradski

Pavlović

Janošević

et al. 2023

Phytochemical Analysis

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Introduction:The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.Materials and Methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzymeinhibiting effects were evaluated against four enzymes. Results:The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition. Conclusion:This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.

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Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers

Cited by 42 publications

References 54 publications

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above‐Ground Vegetative Parts and Flower of Three Iris Species

Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

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