Antibacterial Activity of Desert Truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa

Schillaci, Domenico; Mg, Cusimano; Sm, Cascioferro; Stefano, Vita Di; Arizza, Vincenzo; Chiaramonte, Marco; Inguglia, Luigi; Bawadekji, Abdulhakim; Davino, S C; Ml, Gargano; Venturella, Giuseppe

doi:10.1615/intjmedmushrooms.v19.i2.30

Cited by 21 publications

(11 citation statements)

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“…Subsequently, they were cut with a sterile scalpel, homogenized and kept at −80 • C until following analyses. Cationic peptides were extracted according to well-established protocols [14,[36][37][38][39]. In brief, roots, leaves and bulb freeze-dried samples (1 g/sample) were dissolved in 3 mL of extraction buffer (10% acetic acid in phosphate saline buffer) and sonicated for 60 s at 0 • C (setting: 1 pulse/s, 70% duty cycle).…”

Section: Plant Collection and Plant Extract Preparationmentioning

confidence: 99%

Identification of New Antimicrobial Peptides from Mediterranean Medical Plant Charybdis pancration (Steinh.) Speta

et al. 2020

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The present work was designed to identify and characterize novel antimicrobial peptides (AMPs) from Charybdis pancration (Steinh.) Speta, previously named Urginea maritima, is a Mediterranean plant, well-known for its biological properties in traditional medicine. Polypeptide-enriched extracts from different parts of the plant (roots, leaves and bulb), never studied before, were tested against two relevant pathogens, Staphylococcus aureus and Pseudomonas aeruginosa. With the aim of identifying novel natural AMPs, peptide fraction displaying antimicrobial activity (the bulb) that showed minimum inhibitory concentration (MICs) equal to 30 µg/mL against the above mentioned strains, was analysed by high-resolution mass spectrometry and database search. Seventeen peptides, related to seven proteins present in the investigated database, were described. Furthermore, we focused on three peptides, which due to their net positive charge, have a better chance to be AMPs and they were investigated by molecular modelling approaches, in order to shed light on the solution properties of their equilibrium structures. Some of new detected peptides could represent a good platform for the development of new antimicrobials in the fight against antibiotic resistance phenomenon.

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Section: Plant Collection and Plant Extract Preparationmentioning

confidence: 99%

Identification of New Antimicrobial Peptides from Mediterranean Medical Plant Charybdis pancration (Steinh.) Speta

et al. 2020

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“…Modern scientific data shows that agaricoid, polyporoid, and other taxonomic groups of Macrofungi are producers of a broad spectrum of high-and low-molecular-weight bioactive compounds (BAC), i.e., alkaloids, polysaccharides, proteins, phenolics, terpenoids, polyketides, cyclic peptides, lectins, ribosomeinactivating proteins [21][22][23][24][25][26][27]. These BAC means Bioactive Compounds were investigated for their various therapeutic effects as analgesic, antimicrobial, antiviral, antioxidant, immunomodulatory, anti-inflammatory, antitumor, mitogenic/ regenerative, hypotensive, hepatoprotective, antidiabetic/ hypoglycemic, hypocholesterolemic, cardio-and neuroprotective activities [28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45].…”

Section: Bioactive Compounds and Pharmacological Activity Of Macrofungimentioning

confidence: 99%

Perspectives of Biomedical Application of Macrofungi

Badalyan

Rapior²

2020

CTBEB

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Macrofungi (mushrooms) have widely been appreciated all over the world for their nutritional values and medicinal properties. They have been used in traditional medicine for more than 3000 years for prevention and treatment of different diseases. Modern scientific research shows that macrofungi are producers of a broad spectrum of high-and low-molecular-weight bioactive compounds, i.e., alkaloids, polysaccharides, proteins, phenolics, terpenoids, polyketides, cyclic peptides, lectins and ribosome-inactivating proteins. They have various therapeutic effects as antidiabetic, anti-inflammatory, antimicrobial, antioxidant, antitumor, antiviral, cardioprotective, hepatoprotective, hypocholesterolemic, hypotensive, immunomodulatory, neuroprotective and regenerative, and possess promising pharmacological potential. Development of fungal biotechnological cultivation industry will support production of macrofungi-derived biotech products, healthy food and mycopharmaceuticals pharmaceuticals. Further advances in fungal biology and biotechnology, genomics and proteomics will assist biomedical research and application of macrofungi.

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“…The antibacterial effects of truffle extract, for example, have been well established in the treatment of a variety of diseases, including trachoma [15,16]. Schillaci et al (2017) investigated the antibacterial activities of the edible desert truffle mushrooms Tirmania pinoyi, Terfezia claveryi, and Picoa juniperi against the strains Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) [17]. Paul et al [18] discovered that Terfezia truffles had antifungal efficacy against plant pathogenic fungi.…”

Section: Introductionmentioning

confidence: 99%

Phytonutrients and Antimicrobial Activities of Methanolic Extract from Hafr Al-Batin Truffles

Al-Mazaideh

Alswailmi

2021

JPRI

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The desert truffle is a wild mushroom, also referred to as Kamah or Fagaa. Kamah is a rich source of polysaccharides that have medicinal, antitumoral, antibacterial, and immune-stimulant effects. Studies of hypogeous fungi, especially desert truffles, have recently entered traditional studies of epigeous higher Basidiomycetes. Based on the tasty desert truffle Kamah obtained from Hafr Al-Batin Governorate, Saudi Arabia, as a source of potential antimicrobial agents with both the aim of obtaining novel agents toward bacteria and Fungi of clinical significance. We specifically tested the antibacterial and antifungal efficacy of methanol extracts of Kamah against the Gram-negative bacterial pathogens reference strains E. coli ATCC® 8739, P. Aeruginosa ATCC®9027, S. aureus ATCC®6538, Enterococci NCTC®775 and opportunistic fungus C. albicans ATCC®1231.The extract had MIC (minimum inhibitory concentrations) varying from 100 g/ml to 500 g/ml against the pathogens examined. The LC-QTOF-MS (liquid chromatography coupled to quadrupole time of flight mass spectrometry) phytoconstituents assay chromatogram indicated that the methanol extracts of Kamah comprises 264 with retention periods varying from 1.04 to 18.86, which were categorized as unsaturated and saturated natural ingredients sch as aromatic compounds, carboxylic acids, oxygenated hydrocarbons, fatty acids, amino acids, and vitamins).The main compounds were discovered to be 21 with peak areas larger than 2X10-5 and retention periods varying from 2.3 to 9.13.The main known substances with the maximum peaks were adenosine (11.724), phenylalanine (7.711), phenprobamate (7.711), and 5-hydroxytryptophan (5.711). Such preliminary findings, we assume, are encouraging in terms of obtaining a beneficial antibiotic substitute to battle antibiotic-resistant pathogens especially eye infections.

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Antibacterial Activity of Desert Truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa

Cited by 21 publications

References 0 publications

Identification of New Antimicrobial Peptides from Mediterranean Medical Plant Charybdis pancration (Steinh.) Speta

Identification of New Antimicrobial Peptides from Mediterranean Medical Plant Charybdis pancration (Steinh.) Speta

Perspectives of Biomedical Application of Macrofungi

Phytonutrients and Antimicrobial Activities of Methanolic Extract from Hafr Al-Batin Truffles

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