Effects of gold nanoparticle-based vaccine size on lymph node delivery and cytotoxic T-lymphocyte responses

Kang, Sukmo; Ahn, Sukyung; Lee, Jeewon; Kim, Jin Yong; Choi, Minsuk; Gujrati, Vipul; Kim, Hyungjun; Kim, Jinjoo; Shin, Eui‐Cheol; Jon, Sangyong

doi:10.1016/j.jconrel.2017.04.024

Cited by 125 publications

(91 citation statements)

References 69 publications

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“…Among inorganic nanoparticles, the most commonly used for vaccination are gold nanoparticles (AuNPs). AuNPs are readily internalized by macrophages and dendritic cells, and induce their activation (150,151). Large scale production is possible with strict control on particle size and ease of functionalization using the strong affinity between thiol groups and gold.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Nanoparticle-Based Vaccines Against Respiratory Viruses

et al. 2019

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The respiratory mucosa is the primary portal of entry for numerous viruses such as the respiratory syncytial virus, the influenza virus and the parainfluenza virus. These pathogens initially infect the upper respiratory tract and then reach the lower respiratory tract, leading to diseases. Vaccination is an affordable way to control the pathogenicity of viruses and constitutes the strategy of choice to fight against infections, including those leading to pulmonary diseases. Conventional vaccines based on live-attenuated pathogens present a risk of reversion to pathogenic virulence while inactivated pathogen vaccines often lead to a weak immune response. Subunit vaccines were developed to overcome these issues. However, these vaccines may suffer from a limited immunogenicity and, in most cases, the protection induced is only partial. A new generation of vaccines based on nanoparticles has shown great potential to address most of the limitations of conventional and subunit vaccines. This is due to recent advances in chemical and biological engineering, which allow the design of nanoparticles with a precise control over the size, shape, functionality and surface properties, leading to enhanced antigen presentation and strong immunogenicity. This short review provides an overview of the advantages associated with the use of nanoparticles as vaccine delivery platforms to immunize against respiratory viruses and highlights relevant examples demonstrating their potential as safe, effective and affordable vaccines.

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Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Nanoparticle-Based Vaccines Against Respiratory Viruses

et al. 2019

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“…On day 4, an additional 100 μl of fresh complete cell culture medium without Au-NPs was added to the existing volume. On days 5,6,7,8,11, and 14, 100 μl aliquots were carefully removed from wells and replaced with fresh medium without Au-NPs. Crystal violet staining was performed on days 1 (untreated), 4, 5, 6, 7, 8, 11, and 14.…”

Section: Analysis Of Long-term Cell Growthmentioning

confidence: 99%

“…In recent years, various applications for Au-NPs have emerged as potential players in the field of nanomedicine; for example, as sensitizers in radiation therapy, 3 for bioimaging, [4][5][6] for drug and gene delivery, 7,8 as novel strategy for chemotherapy by conjugation to conventional drugs, 9,10 or as nanoparticle-based vaccines. 11,12 For the safe and efficient use of gold-based nanomedicines, assessment of their potential side effects is required as a sound basis for appropriate risk-benefit analyses. To date, there have been various reports, often with contradictory findings regarding the cyto-and genotoxicity of Au-NPs in vitro and in vivo; however, these are insufficient for comprehensive hazard assessment.…”

Section: Introductionmentioning

confidence: 99%

Transient DNA damage following exposure to gold nanoparticles

et al. 2018

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Due to their interesting physicochemical properties, gold nanoparticles (Au-NPs) are the focus of increasing attention in the field of biomedicine and are under consideration for use in drug delivery and bioimaging, or as radiosensitizers and nano-based vaccines. Thorough evaluation of the genotoxic potential of Au-NPs is required, since damage to the genome can remain undetected in standard hazard assessments. Available genotoxicity data is either limited or contradictory. Here, we examined the influence of three surface modified 3-4 nm Au-NPs on human A549 cells, according to the reactive oxygen species (ROS) paradigm. After 24 h of Au-NP treatment, nanoparticles were taken up by cells as agglomerates; however, no influence on cell viability or inflammation was detected. No increase in ROS production was observed by H2-DCF assay; however, intracellular glutathione levels reduced over time, indicating oxidative stress. All three types of Au-NPs induced DNA damage, as detected by alkaline comet assay. The strongest genotoxic effect was observed for positively charged Au-NP I. Further analysis of Au-NP I by neutral comet assay, fluorimetric detection of alkaline DNA unwinding assay, and γH2AX staining, revealed that the induced DNA lesions were predominantly alkali-labile sites. As highly controlled repair mechanisms have evolved to remove a wide range of DNA lesions with great efficiency, it is important to focus on both acute cyto- and genotoxicity, alongside post-treatment effects and DNA repair. We demonstrate that Au-NP-induced DNA damage is largely repaired over time, indicating that the observed damage is of transient nature.

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“…One of the principal differences with other nanoparticles, in particular with liposomes, is the stability of the resulting formulations. The critical micelle concentration (CMC), defined as the concentration of surfactants above which the micelles are spontaneous formed, increases in the presence of salts with consequently breakdown the micelles' structures [48]. Moreover, in the blood stream micellar formulations are diluted in blood and tend to dissociate into monomers with immediate drug release.…”

Section: Polimeric Micellesmentioning

confidence: 99%

Nanoparticles to Improve the Efficacy of Peptide-Based Cancer Vaccines

Tornesello

Tagliamonte

Tornesello

et al. 2020

Cancers

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Nanoparticles represent a potent antigen presentation and delivery system to elicit an optimal immune response by effector cells targeting tumor-associated antigens expressed by cancer cells. Many types of nanoparticles have been developed, such as polymeric complexes, liposomes, micelles and protein-based structures such as virus like particles. All of them show promising results for immunotherapy approaches. In particular, the immunogenicity of peptide-based cancer vaccines can be significantly potentiated by nanoparticles. Indeed, nanoparticles are able to enhance the targeting of antigen-presenting cells (APCs) and trigger cytokine production for optimal T cell response. The present review summarizes the categories of nanoparticles and peptide cancer vaccines which are currently under pre-clinical evaluation.

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Effects of gold nanoparticle-based vaccine size on lymph node delivery and cytotoxic T-lymphocyte responses

Cited by 125 publications

References 69 publications

Nanoparticle-Based Vaccines Against Respiratory Viruses

Nanoparticle-Based Vaccines Against Respiratory Viruses

Transient DNA damage following exposure to gold nanoparticles

Nanoparticles to Improve the Efficacy of Peptide-Based Cancer Vaccines

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