Identification and Characterization of microRNA319a and Its Putative Target Gene, PvPCF5, in the Bioenergy Grass Switchgrass (Panicum virgatum)

Xie, Qi; Li, Xue; Zhang, Yinbing; Tang, Jian; Yin, Dedong; Bo, Fan; Zhu, Lihuang; Han, Liebao; Song, Guilong; Li, Dayong

doi:10.3389/fpls.2017.00396

Cited by 14 publications

(7 citation statements)

References 44 publications

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“…All TCP proteins contain a highly conserved TCP domain which is a 59-amino-acid non-canonical basic-helix-loop-helix (bHLH) motif at the N-terminus and functions in DNA binding, protein–protein interaction, and protein nuclear localization ( Kosugi and Ohashi, 2002 ; Aggarwal et al, 2010 ; Martin-Trillo and Cubas, 2010 ; Dhaka et al, 2017 ). So far, TCP genes have been identified in various plant species including Arabidopsis , rice, tomato, tobacco, and strawberry as shown in Supplementary Figure S1 ( Palatnik et al, 2003 ; Li et al, 2005 ; Aguilar-Martinez et al, 2007 ; Ori et al, 2007 ; Yao et al, 2007 ; Schommer et al, 2008 ; Nag et al, 2009 ; Giraud et al, 2010 ; Koyama et al, 2010b ; Sarvepalli and Nath, 2011 ; Yang et al, 2012 ; Ma et al, 2014 ; Parapunova et al, 2014 ; Wang et al, 2015b ; Chen et al, 2016 ; Lin et al, 2016 ; Ma et al, 2016 ; Wei et al, 2016 ; Zhou et al, 2016 ; Du et al, 2017 ; Xie et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of TCP Family Transcription Factors in Medicago truncatula Reveals Significant Roles of miR319-Targeted TCPs in Nodule Development

Wang

Liu

et al. 2018

Front. Plant Sci.

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TCP proteins, the plant-specific transcription factors, are involved in the regulation of multiple aspects of plant development among different species, such as leaf development, branching, and flower symmetry. However, thus far, the roles of TCPs in legume, especially in nodulation are still not clear. In this study, a genome-wide analysis of TCP genes was carried out to discover their evolution and function in Medicago truncatula. In total, 21 MtTCPs were identified and classified into class I and class II, and the class II MtTCPs were further divided into two subclasses, CIN and CYC/TB1. The expression profiles of MtTCPs are dramatically different. The universal expression of class I MtTCPs was detected in all organs. However, the MtTCPs in CIN subclass were highly expressed in leaf and most of the members in CYC/TB1 subclass were highly expressed in flower. Such organ-specific expression patterns of MtTCPs suggest their different roles in plant development. In addition, most MtTCPs were down-regulated during the nodule development, except for the putative MtmiR319 targets, MtTCP3, MtTCP4, and MtTCP10A. Overexpression of MtmiR319A significantly reduced the expression level of MtTCP3/4/10A/10B and resulted in the decreased nodule number, indicating the important roles of MtmiR319-targeted MtTCPs in nodulation. Taken together, this study systematically analyzes the MtTCP gene family at a genome-wide level and their possible functions in nodulation, which lay the basis for further explorations of MtmiR319/MtTCPs module in association with nodule development in M. truncatula.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of TCP Family Transcription Factors in Medicago truncatula Reveals Significant Roles of miR319-Targeted TCPs in Nodule Development

Wang

Liu

et al. 2018

Front. Plant Sci.

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“…Furthermore, to verify the direct interaction between miR319 and OsTCP21 and OsGAmyb , respectively, we used a rapid transient assay in Nicotiana benthamiana which has been reported previously (Xie et al, 2017; Palatnik and Weigel, 2019). The results showed that the tobacco leaves generated GFP fluorescence injecting with 35S: OsTCP21 ‐GFP, 35S: OsGAmyb ‐GFP or empty vectors with GFP (35S:GFP) respectively, whereas co‐expression with 35S: MIR319a greatly reduced the GFP signal (Figure S3).…”

Section: Resultsmentioning

confidence: 99%

MiR319‐targeted OsTCP21 and OsGAmyb regulate tillering and grain yield in rice

Wang

Yang

Guo

et al. 2021

JIPB

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Multiple genes and microRNAs (miRNAs) improve grain yield by promoting tillering. MiR319s are known to regulate several aspects of plant development; however, whether miR319s are essential for tillering regulation remains unclear. Here, we report that miR319 is highly expressed in the basal part of rice plant at different development stages. The miR319 knockdown line Short Tandem Target Mimic 319 (STTM319) showed higher tiller bud length in seedlings under low nitrogen (N) condition and higher tiller bud number under high N condition compared with the miR319a‐overexpression line. Through targets prediction, we identified OsTCP21 and OsGAmyb as downstream targets of miR319. Moreover, OsTCP21 and OsGAmyb overexpression lines and STTM319 had increased tiller bud length and biomass, whereas both were decreased in OsTCP21 and OsGAmyb knockout lines and OE319a. These data suggest that miR319 regulates rice tiller bud development and tillering through targeting OsTCP21 and OsGAmyb. Notably, the tiller number and grain yield increased in STTM319 and overexpression lines of OsTCP21 and OsGAmyb but decreased in OE319a and knockout lines of OsTCP21 and OsGAmyb. Taken together, our findings indicate that miR319s negatively affect tiller number and grain yield by targeting OsTCP21 and OsGAmyb, revealing a novel function for miR319 in rice.

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“…To increase fermentable sugar or yield of biofuels, it requires to improve the relative amount of cell wall components, like cellulose or non-structural carbohydrates, reduced lignin content, and expression of endogenous hydrolyzing proteins that support more saccharification process [148,149]. Recently, large numbers of miRNAs and genes have been examined, which regulate cell wall synthesis, compositions, and bio-recalcitrance in a wide variety of plants [129,148,150]. It is well known that bio-recalcitrance is a major limiting factor that is mainly responsible for up to 45-55% total cost of biofuel production [108,151].…”

Section: The Role Of Mir156 In the Reduction Of Bio-recalcitrancementioning

confidence: 99%

The miR156: A Master Regulator to Harmonize Complex Biofuel Traits in Lignocellulosic Biomass Crops

Singh¹

2020

Preprint

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Currently, energy security and environmental degradation are the two biggest challenges before humanity that can be surmounted with the use of green and sustainable biofuels produced from lignocellulosic crops. In the future, to ensure adequate and cost-effective supply of biofuels, it requires a sufficient amount of amenable and quality lignocellulosic feedstocks. Therefore, agricultural yields of lignocellulosic biomass crops should be substantially increased by intense genetic maneuvering of key gene regulatory mechanisms and signaling pathways that control plant biomass yield. Recently, numerous miRNAs families are identified, characterized, and validated across the plant kingdom. Plant microRNAs (miRNAs) are 21 to 24 nucleotides long, non-coding small RNAs, act as regulators of their target genes via inducing modifications in transcription, translation, and epigenome. MiRNAs represent many hallmark characteristics like sequence-specific regulation, tissue, and species-specific expression, evolutionary conservation, and functional diversity. They coordinate well physiological and life cycle processes in plants under adverse environmental conditions. Hence, miRNAs offer accurate, precise, and efficient regulatory switches in the miRNA-targeted genetic networks. It is evident from the study of the miR156 family and its target SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes network that controls highly significant agronomic traits in crop plants. The miR156/SPL module acts as a master circuit that synchronizes many intricate complex biological functions such as growth and development, and metabolic processes by sensing internal and external environmental signals in plants. Therefore, miR156 can prove a potential target for miRNAs based plant biotechnology to harmonize complex biofuel traits and improve biomass yield in lignocellulosic biomass crops.

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Identification and Characterization of microRNA319a and Its Putative Target Gene, PvPCF5, in the Bioenergy Grass Switchgrass (Panicum virgatum)

Cited by 14 publications

References 44 publications

Genome-Wide Identification of TCP Family Transcription Factors in Medicago truncatula Reveals Significant Roles of miR319-Targeted TCPs in Nodule Development

Genome-Wide Identification of TCP Family Transcription Factors in Medicago truncatula Reveals Significant Roles of miR319-Targeted TCPs in Nodule Development

MiR319‐targeted OsTCP21 and OsGAmyb regulate tillering and grain yield in rice

The miR156: A Master Regulator to Harmonize Complex Biofuel Traits in Lignocellulosic Biomass Crops

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