Bladder smooth muscle cells on electrospun poly(ε-caprolactone)/poly( l -lactic acid) scaffold promote bladder regeneration in a canine model

Shakhssalim, Nasser; Soleimani, Masoud; Rasouli, Javad; Taghizadeh-Jahed, Masoud; Torbati, Peyman Mohammadi; Naji, Mohammad

doi:10.1016/j.msec.2017.02.064

Cited by 24 publications

(21 citation statements)

References 24 publications

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“…Shakhssalim et al () succeeded in applying electrospinning to generate poly(ε‐caprolactone)/poly(l‐lactic acid) scaffolds (10 cm 2 ) for canine bladder wall replacement. Cellular matrices supported the maintenance of seeded detrusor SMCs, which in vivo stimulated the growth of local native cells.…”

Section: Tissue Engineering Strategies For Urinary Tract Reconstructionmentioning

confidence: 99%

Reconstructive urology and tissue engineering: Converging developmental paths

Adamowicz

Kuffel

Breda

et al. 2019

J Tissue Eng Regen Med

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Reconstructive urology is a complex and demanding branch of modern urology.Complicated cases, necessity of microsurgical approach, and constant exposure to urine make urinary reconstruction especially difficult. With impaired healing, excessive scarring, and recurring fibrosis, functional results are still not satisfying.For better, more successful outcomes, a novel tissue engineering technology-based solutions are being gradually investigated. The use of tissue engineering is the most promising strategy to improve results of reconstructive urology procedures due to possibility of designing organ-specific grafts. Moreover, targeted modification of healing environment by stem cells and growth factors is a unique opportunity that might bring reconstructive urology on molecular level. This review defined limitations and problems encountered in reconstructive urology and discussed relevant tissue engineering-based achievements in the field of urethra, urinary bladder, and ureter regeneration. The background justifying tissue engineering approach to urethra, urinary bladder, and ureter reconstruction was discussed. Then, the wide range of experimental methods utilising biomaterials and cell seeding was deliberated to show readers the current tools offered by tissue engineering. At the end, we characterised major challenges that are needed to be addressed before tissue entering would become standard technology in urological departments.

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Section: Tissue Engineering Strategies For Urinary Tract Reconstructionmentioning

confidence: 99%

Reconstructive urology and tissue engineering: Converging developmental paths

Adamowicz

Kuffel

Breda

et al. 2019

J Tissue Eng Regen Med

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“…Substantial research is currently focused on the potential utility of electrospun scaffolds for clinical applications including the repair of diaphragm [1], bladder [2][3][4], and ligaments [5][6][7][8], as well as grafting procedures for bone [5,9], skin [10][11][12], and vascular [13,14] tissue. Electrospun scaffolds provide a 3-dimensional fibrous matrix with interconnecting pores, a feature that mimics the native extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

et al. 2019

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Background: There is substantial interest in electrospun scaffolds as substrates for tissue regeneration and repair due to their fibrous, extracellular matrix-like composition with interconnected porosity, cost-effective production, and scalability. However, a common limitation of these scaffolds is their inherently low mechanical strength and stiffness, restricting their use in some clinical applications. In this study we developed a novel technique for 3D printing a mesh reinforcement on electrospun scaffolds to improve their mechanical properties. Methods: A poly (lactic acid) (PLA) mesh was 3D-printed directly onto electrospun scaffolds composed of a 40:60 ratio of poly(ε-caprolactone) (PCL) to gelatin, respectively. PLA grids were printed onto the electrospun scaffolds with either a 6 mm or 8 mm distance between the struts. Scanning electron microscopy was utilized to determine if the 3D printing process affected the archtitecture of the electrospun scaffold. Tensile testing was used to ascertain mechanical properties (strength, modulus, failure stress, ductility) of both unmodified and reinforced electrospun scaffolds. An in vivo bone graft model was used to assess biocompatibility. Specifically, reinforced scaffolds were used as a membrane cover for bone graft particles implanted into rat calvarial defects, and implant sites were examined histologically. Results: We determined that the tensile strength and elastic modulus were markedly increased, and ductility reduced, by the addition of the PLA meshes to the electrospun scaffolds. Furthermore, the scaffolds maintained their matrix-like structure after being reinforced with the 3D printed PLA. There was no indication at the graft/tissue interface that the reinforced electrospun scaffolds elicited an immune or foreign body response upon implantation into rat cranial defects. Conclusion: 3D-printed mesh reinforcements offer a new tool for enhancing the mechanical strength of electrospun scaffolds while preserving the advantageous extracellular matrix-like architecture. The modification of electrospun scaffolds with 3D-printed reinforcements is expected to expand the range of clinical applications for which electrospun materials may be suitable.

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“…16,17 As an example, Shakhssalim et al explored the application of electrospun PLA/PCL scaffolds for bladder regeneration by culturing smooth muscle and urothelial cells. 18 Moreover, electrospinning as a means of generating porous structures has many advantages:…”

Section: Introductionmentioning

confidence: 99%

PLA/PCL electrospun membranes of tailored fibres diameter as drug delivery systems

Herrero-Herrero

Gómez-Tejedor

Vallés-Lluch

2018

European Polymer Journal

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The main electrospinning parameters, i.e., polymer concentration in the injectable solution, solvents used and their proportion, flow rate, voltage and distance to collector were herein systematically modified to analyse their particular influence in fibres diameter of electrospun membranes of poly(lactic acid), polycaprolactone and their mixture. As a result of this analysis, the procedures to obtain membranes of these polymers and blend with under-and above-micron-sized fibres were established, in which the solvents ratio (chloroform/methanol and dichloromethane/dimethylformamide) and voltage were found to play the major role. Moreover, the plausible differential effect of these fibres diameters (0.8 and 1.8 µm) in the controlled release of a molecule of interest was explored, using bovine serum albumin (BSA), proving that the most effective configuration for BSA release among those studied was the PLA-PCL combination in membranes of above-micron fibres diameter.

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Bladder smooth muscle cells on electrospun poly(ε-caprolactone)/poly( l -lactic acid) scaffold promote bladder regeneration in a canine model

Cited by 24 publications

References 24 publications

Reconstructive urology and tissue engineering: Converging developmental paths

Reconstructive urology and tissue engineering: Converging developmental paths

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

PLA/PCL electrospun membranes of tailored fibres diameter as drug delivery systems

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