Role of EGF on in situ culture of equine preantral follicles and metabolomics profile

Aguiar, F.L.N.; Lunardi, Franciele Osmarini; Lima, Laritza Ferreira de; Bruno, Jamily Bezerra; Alves, Bênner Geraldo; Magalhães-Padilha, D.M.; Cibin, Francielli Weber Santos; Berioni, L.; Apgar, G. A.; Turco, E.G. Lo; Gastal, E.L.; Figueiredo, J.R.

doi:10.1016/j.rvsc.2017.04.001

Cited by 20 publications

(9 citation statements)

References 63 publications

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“…Regarding follicular and oocyte diameter, all treatments reduced the follicular and oocyte diameters compared to the control group. Similar results were reported in goats (Silva et al, 2002) and mares (Aguiar et al, 2017a). Considering that follicular and oocyte diameters were only recorded using MNF, the diameter decreasing during culture is probably due to the atresia of more advance follicle stages (i.e., primary and secondary) that are more likely to undergo atresia than primordial follicles (Aguiar et al, 2016).…”

Section: Discussionsupporting

confidence: 80%

Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue

et al. 2019

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This study evaluated a powdered coconut water solution (ACP 406 ) as a base culture medium on the in vitro survival and development of in situ goat preantral follicles. The ovarian fragments were either immediately fixed in Carnoy solution (non-cultured control) or individually cultured for 2 or 6 days. The following culture media (all containing 100 μg/mL penicillin and 100 μg/mL streptomycin) were evaluated: α-MEM (α-MEM alone, without additional supplementation); α-MEM+ (supplemented α-MEM); ACP (ACP  406 alone); or ACP+ (supplemented ACP  406). Additional supplementation includes: 1.25 mg/mL bovine serum albumin, 10 μg/mL insulin, 5.5 μg/mL transferrin, 5 ng/mL selenium, 2 mM glutamine, and 2 mM hypoxanthine. The endpoints (i) follicular morphology; (ii) development; (iii) estradiol production; and (iv) reactive oxygen species (ROS) were recorded. Data were analyzed using chi-square, Turkey, t-test or One-Way ANOVA. Differences were considered significant when P < 0.05. At day 2 of culture, a greater (P < 0.05) percentage of morphologically normal follicles was observed between ACP+ and ACP treatments. Moreover, at day 2 of culture, no hormonal difference (P < 0.05) was observed between ACP+ and both α-MEM treatments. At day 6 of culture when ACP and α-MEM treatments were compared the percentage of healthy follicles were similar (P > 0.05) among treatments. Overall, all treatments had lower primordial follicles (P < 0.05) accompany by greater developing follicles (P < 0.05) percentages than non-cultured control treatment, indicating primordial follicle activation. However, at day 6 of culture, the percentage of primordial follicle development were similar (P > 0.05) among the treatments. Likewise, no differences (P > 0.05) were observed for ROS production and follicular and oocyte diameters among treatments. Therefore, ACP+ has the equivalent efficiency to MEM+ in maintaining the survival and development of goat preantral follicles, representing an alternative plant-based low-cost culture medium for in vitro culture.

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Section: Discussionsupporting

confidence: 80%

Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue

et al. 2019

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“…During the in vitro culture process, part of the ovarian follicles become atretic, especially more advanced follicle stage (primary and secondary) ( Meresman, 2011 ). However, most of the ovarian follicles measured in the current experiment, after in vitro culture, were intermediate which are, in general, smaller than the aforementioned follicular categories ( Aguiar et al., 2017 ). This may explain the decrease in follicle and oocyte diameter in this study.…”

Section: Discussionmentioning

confidence: 50%

Immunolocalization for glucocorticoid receptor and effect of cortisol on in vitro development of preantral follicles

Pontes

Maside

Lima

et al. 2019

Veterinary and Animal Science

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Highlights In this study, the immunolocalization for glucocorticoid receptor (NR3C1) in goat ovarian follicles and the effect of cortisol on in vitro development of preantral follicles was evaluate. The NR3C1 was strongly expressed in oocytes of primary and antral follicles. A progressive increase of immunostaining for NR3C1 in granulosa cells from primordial to antral follicles was observed. In conclusion, it was observed the presence of NR3C1 in the oocyte and granulosa cells in all follicular categories. The in vitro culture showed that high cortisol concentration (10 ng/ml) exerts a deleterious effect on follicular survival.

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“…The use of heterotopic autotransplantation and xenotransplantation in livestock may become a powerful tool for (1) treatment of infertility caused by ovarian abnormalities (e.g., benign tumors and adhesions) and oviductal and uterine disorders, and (2) making easier the recovery of immature or mature oocytes from females of high genetic value that need preservation. In the area of preantral follicle research in horses, several studies have been conducted recently on ovarian tissue storage and cryopreservation [12][13][14], in vitro follicle culture [15,16], and preantral follicle population, addressing aspects related to aging effect [17], follicular dynamics [11], and spatial follicle distribution [18].…”

Section: Introductionmentioning

confidence: 99%

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

Souza

Alves

et al. 2020

PLoS ONE

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Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation technique in a large livestock species (i.e., horses) after ovarian fragments were exposed or not to a cooling process (4°C/24 h) and/or VEGF before grafting. Ovarian fragments were collected in vivo via an ultrasound-guided biopsy pick-up method and surgically autografted in a subcutaneous site in both sides of the neck in each mare. The blood flow perfusion at the transplantation site was monitored at days 2, 4, 6, and 7 post-grafting using color-Doppler ultrasonography. Ovarian grafts were recovered 7 days post-transplantation and subjected to histological analyses. The exposure of the ovarian fragments to VEGF before grafting was not beneficial to the quality of the tissue; however, the cooling process of the fragments reduced the acute hyperemia post-grafting. Cooled grafts compared with non-cooled grafts contained similar values for normal and developing preantral follicles, vessel density, and stromal cell apoptosis; lower collagen type III fibers and follicular density; and higher stromal cell density, AgNOR, and collagen type I fibers. In conclusion, VEGF exposure before autotransplantation did not improve the quality of grafted tissues. However, cooling ovarian tissue for at least 24 h before grafting can be beneficial because satisfactory rates of follicle survival and development, stromal cell survival and proliferation, as well as vessel density, were obtained.

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Role of EGF on in situ culture of equine preantral follicles and metabolomics profile

Cited by 20 publications

References 63 publications

Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue

Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue

Immunolocalization for glucocorticoid receptor and effect of cortisol on in vitro development of preantral follicles

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF

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