Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination

Zhang, Hong; Zhou, Kexin; Wang, Weiqing; Liu, Shujun; Song, Song-Quan

doi:10.1016/j.jplph.2017.03.008

Cited by 7 publications

(4 citation statements)

References 75 publications

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“…Germination is a process influenced by many genetic, endogenous, but also environmental factors, and consists of a series of cellular processes which produce various changes in the structure of the cell, some being visible to the naked eye. The germination process is being studied even today to fully elucidate its mechanism [29]. Some researchers have reported that the germination process, used for plant reproduction, is influenced not only by abiotic factors, but also by biotic stressors, such as pathogens and infestants [30].…”

Section: Description Of the Germination Processmentioning

confidence: 99%

Perspectives on the Use of Germinated Legumes in the Bread Making Process, A Review

Atudorei

Codină

2020

Applied Sciences

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Nowadays, it may be noticed that there is an increased interest in using germinated seeds in the daily diet. This high interest is due to the fact that in a germinated form, the seeds are highly improved from a nutritional point of view with multiple benefits for the human body. The purpose of this review was to update the studies made on the possibilities of using different types of germinated legume seeds (such as lentil, chickpea, soybean, lupin, bean) in order to obtain bakery products of good quality. This review highlights the aspects related to the germination process of the seeds, the benefits of the germination process on the seeds from a nutritional point of view, and the effects of the addition of flour from germinated seeds on the rheological properties of the wheat flour dough, but also on the physico–chemical and sensory characteristics of the bakery products obtained. All these changes on the bread making process and bread quality depend on the level and type of legume seed subjected to the germination process which are incorporated in wheat flour.

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Section: Description Of the Germination Processmentioning

confidence: 99%

Perspectives on the Use of Germinated Legumes in the Bread Making Process, A Review

Atudorei

Codină

2020

Applied Sciences

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“…Previous research on rapid asexual propagation and tissue culture of poplar has been highly successful [30, 31]. In comparison, few studies have been carried out on molecular mechanisms of poplar seed germination (sexual reproduction), although several preliminary reports on the relationship between seed germination and environment have recently appeared [28, 32–34]. Investigation of the molecular mechanism of poplar seed germination and its immediate aftermath may be key to the elucidation of this process in woody plants.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive dissection of transcript and metabolite shifts during seed germination and post-germination stages in poplar

Zuo

Cao

et al. 2019

BMC Plant Biol

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Background Seed germination, a complex, physiological–morphogenetic process, is a critical stage in the life cycle of plants. Biological changes in germinating seeds have not been investigated in poplar, a model woody plant. Results In this study, we exploited next-generation sequencing and metabolomics analysis and uncovered a series of significantly different genes and metabolites at various stages of seed germination and post germination. The K -means method was used to identify multiple transcription factors, including AP2/EREBP , DOF , and YABBY , involved in specific seed germination and post-germination stages. A weighted gene coexpression network analysis revealed that cell wall, amino acid metabolism, and transport-related pathways were significantly enriched during stages 3 and 5, with no significant enrichment observed in primary metabolic processes such as glycolysis and the tricarboxylic acid cycle. A metabolomics analysis detected significant changes in intermediate metabolites in these primary metabolic processes, while a targeted correlation network analysis identified the gene family members most relevant to these changing metabolites. Conclusions Taken together, our results provide important insights into the molecular networks underlying poplar seed germination and post-germination processes. The targeted correlation network analysis approach developed in this study can be applied to search for key candidate genes in specific biochemical reactions and represents a new strategy for joint multiomics analyses. Electronic supplementary material The online version of this article (10.1186/s12870-019-1862-3) contains supplementary material, which is available to authorized users.

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“…Asexual reproduction in poplar has been widely studied, and the experimental techniques are quite reliable [18,19]. In contrast, few reports have appeared on molecular mechanisms of poplar seed germination [15,[20][21][22]. We previously studied specific genes at different stages of poplar seed germination by weighted gene co-expression network analysis (WGCNA) and other methods and identified some candidate genes possibly playing important roles at certain germination stages.…”

Section: Discussionmentioning

confidence: 99%

The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination

Zhao

Chen

et al. 2019

BMC Genomics

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Background: Seed germination, the foundation of plant propagation, involves a series of changes at the molecular level. Poplar is a model woody plant, but the molecular events occurring during seed germination in this species are unclear. Results: In this study, we investigated changes in gene transcriptional levels during different germination periods in poplar by high-throughput sequencing technology. Analysis of genes expressed at specific germination stages indicated that these genes are distributed in many metabolic pathways. Enrichment analysis of significantly differentially expressed genes based on hypergeometric testing revealed that multiple pathways, such as pathways related to glycolysis, lipid, amino acid, protein and ATP synthesis metabolism, changed significantly at the transcriptional level during seed germination. A comparison of ΣZ values uncovered a series of transcriptional changes in biological processes related to primary metabolism during poplar seed germination. Among these changes, genes related to CHO metabolism were the first to be activated, with subsequent expression of genes involved in lipid metabolism and then those associated with protein metabolism. The pattern of metabolomic and physiological index changes further verified the sequence of some biological events. Conclusions: Our study revealed molecular events occurring at the transcriptional level during seed germination and determined their order. These events were further verified by patterns of changes of metabolites and physiological indexes. Our findings lay a foundation for the elucidation of the molecular mechanisms responsible for poplar seed germination.

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Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination

Cited by 7 publications

References 75 publications

Perspectives on the Use of Germinated Legumes in the Bread Making Process, A Review

Perspectives on the Use of Germinated Legumes in the Bread Making Process, A Review

Comprehensive dissection of transcript and metabolite shifts during seed germination and post-germination stages in poplar

The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination

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