The effect of thermosensitive liposomal formulations on loading and release of high molecular weight biomolecules

Adapting liposomes for oral drug delivery

“…The clearing occurs by plasma opsonization and subsequent sequestration from circulation5., 6., 7., 8.. By pegylation, a process of coating with long-chain polyethylene glycols (PEG), liposomes are camouflaged with layers of hydrophilic coatings to evade RES clearance and achieve long circulation in the body9., 10., 11., 12., 13., 14., 15., 16.. The successful marketing of Doxil ® , a pegylated liposomal doxorubicin product, represents a milestone in the development of parenteral liposomes 17 .…”

Understanding the Nano-bio Interfaces: Lipid-Coatings for Inorganic Nanoparticles as Promising Strategy for Biomedical Applications

“…Within the liposomes, the lipids act as protective barrier for the loaded molecular cargo and ensures its stability. The great advantage of liposomes is that the molecule loading is completely reversible and the loaded active molecules can be released once the liposomes reached the target organ or tissue (Huang et al, 2017). Liposomes have been successfully implemented for transporting and delivering drugs or probes for both therapeutic and imaging applications (Vitiello et al, 2015; Irace et al, 2017).…”

Nanosized Delivery Systems for Therapeutic Proteins: Clinically Validated Technologies and Advanced Development Strategies

“…Liposomes are phospholipid vesicles which consist of at least one lipid bilayer enclosing a discrete aqueous domain. While hydrophobic compounds can be inserted into the lipid membrane, hydrophilic molecules can be entrapped in the aqueous core, and this characteristic enables low and high molecular weight biomolecules to be encapsulated and later released at the targeted site (Sercombe et al, 2015;Huang et al, 2017). Liposomes represent the first nanosized drug delivery system which made the transition from bench to clinical application, and provide ideal characteristics of biocompatibility, biodegradability, variable compositions (Allen and Cullis, 2013;Sercombe et al, 2015).…”

“…However, compared with protein-polymer conjugates, a limited quantity of proteinloaded liposomes has been approved for marketing, and the majority of current liposomal protein formulations are still in preclinical stages (Table 2). In fact, although liposomes are good candidates for in vivo delivery of high molecular weight compounds (such as protein/peptide drugs and nucleic acids), their nanoencapsulation is often hindered by instability issues during the liposome preparative process and storage, as well as by the low encapsulation efficiencies (Xu et al, 2012;Huang et al, 2017), as discussed hereafter.…”

“…Several lipids present a low-temperature transition, DPPC is the most common thermosensitive lipid which presents a Tm close to 41 • C (Mazzotta et al, 2018). DSPE-PEG2000 also helped to stabilize the lipid membrane at physiological temperature and to enhance the kinetics release at 40-41 • C (from 10 to 40% after 2 h incubation) (Huang et al, 2017). Listeriolysin O-loaded thermosensitive immunoliposomes were developed to release the payload when heated slightly above body temperature (Kullberg et al, 2005).…”