In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide

Vinardell, M. Pilar; Llanas, Hector; Marics, Laura; Mitjans, Montserrat

doi:10.3390/nano7030056

Cited by 53 publications

(29 citation statements)

References 21 publications

(22 reference statements)

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“…Vinardell et al reported the IC50 values of ZnO (50 nm) and ZnO (100 nm) nanoparticles to be around 50 and 40 µg/mL, respectively, in HaCaT cells after a 48-hour incubation period. 35 Genç et al also reported that nanoscale to microscale ZnO particles showed lower toxicity in HaCaT cells than ZnO nanoparticles (,100 nm in diameter) with/ without UV-A and UV-B. 36 Compared to TiO 2 nanoparticle toxicity, TiO 2 nanoparticles had an autophagic effect on HaCaT cells in a dose-dependent manner even at noncytotoxic levels.…”

Section: Discussionmentioning

confidence: 99%

<p>Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection</p>

Jin

Hwang³

et al. 2019

IJN

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Background: Zinc oxide (ZnO) nanoparticles and their networks have been developed for use in various applications such as gas sensors and semiconductors. Aim: In this study, their antibacterial activity against Escherichia coli under dual ultraviolet (UV) irradiation for disinfection was investigated. Materials and methods: ZnO nanoparticles were synthesized and immobilized onto silicon (Si) wafers by self-assembly. The physicochemical properties and antibacterial activity of ZnO nanoparticles and their networks were evaluated. Gene ontology was analyzed and toxicity levels were also monitored. Results: Synthesized ZnO nanoparticles were spherical nanocrystals (,100 nm; Zn, 47%; O, 53%) that formed macro-mesoporous three-dimensional nanostructures on Si wafers in a concentration-dependent manner. ZnO nanoparticles and their networks on Si wafers had an excellent antibacterial activity against E. coli under dual UV irradiation (.3log CFU/mL). Specifically, arrayed ZnO nanoparticle networks showed superior activity compared with free synthesized ZnO nanoparticles. Oxidative stress-responsive proteins in E. coli were identified and categorized, which indicated antibacterial activity. Synthesized ZnO nanoparticles were less cytotoxic in HaCaT with an IC50 of 6.632 mg/mL, but phototoxic in Balb/c 3T3. Conclusion: The results suggested that ZnO nanoparticles and their networks can be promising photocatalytic antibiotics for use in next-generation disinfection systems. Their application could also be extended to industrial and clinical use as effective and safe photocatalytic antibiotics.

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Section: Discussionmentioning

confidence: 99%

<p>Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection</p>

Jin

Hwang³

et al. 2019

IJN

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“…24,25 In further studies, ZnONPs have reduced cell viability in dose-dependent and time-dependent manner. 26 ZnONPs are suspected of increasing the expression of the metallothionein gene, which is considered a biomarker in metal-induced toxicity. 27 Studies have confirmed the dose-dependent hepatotoxicity and significant increase in oxidative stress through an increase in malondial- dehyde (MDA) content and decrease in superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes activity in the liver.…”

Section: Nanomaterials In Daily Life and Their Toxicitymentioning

confidence: 99%

Nanoparticles in Daily Life: Applications, Toxicity and Regulations

Gupta

Xie

2018

J Environ Pathol Toxicol Oncol

384

188

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At nanoscale, man-made materials may show unique properties that differ from bulk and dissolved counterparts. The unique properties of engineered nanomaterials not only impart critical advantages but also confer toxicity because of their unwanted interactions with different biological compartments and cellular processes. In this review, we discuss various entry routes of nanomaterials in the human body, their applications in daily life, and the mechanisms underlying their toxicity. We further explore the passage of nanomaterials into air, water, and soil ecosystems, resulting in diverse environmental impacts. Briefly, we probe the available strategies for risk assessment and risk management to assist in reducing the occupational risks of potentially hazardous engineered nanomaterials including the control banding (CB) approach. Moreover, we substantiate the need for uniform guidelines for systematic analysis of nanomaterial toxicity, in silico toxicological investigations, and obligation to ensure the safe disposal of nanowaste to reduce or eliminate untoward environmental and health impacts. At the end, we scrutinize global regulatory trends, hurdles, and efforts to develop better regulatory sciences in the field of nanomedicines.

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“…RHE models are used for dermal exposure to cosmetics and environmental irritants and pollutants with approved OECD guidelines like the in vitro skin irritation assay [ 23 ]. Such an approach has been used to determine the extent of penetration and the toxicity of several nanomaterials [ 24 , 25 ], but not yet for AgNW.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

et al. 2018

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Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies that have specifically evaluated the potential toxicity from dermal exposure. To address this question, we investigated the dermal toxicity after acute exposure of polymer-coated AgNW with two sizes using two models, human primary keratinocytes and human reconstructed epidermis. In keratinocytes, AgNW are rapidly and massively internalized inside cells leading to dose-dependent cytotoxicity that was not due to Ag+ release. Analysing our data with different dose metrics, we propose that the number of NW is the most appropriate dose-metric for studies of AgNW toxicity. In reconstructed epidermis, the results of a standard in vitro skin irritation assay classified AgNW as non-irritant to skin and we found no evidence of penetration into the deeper layer of the epidermis. The findings show that healthy and intact epidermis provides an effective barrier for AgNW, although the study does not address potential transport through follicles or injured skin. The combined cell and tissue model approach used here is likely to provide an important methodology for assessing the risks for skin exposure to AgNW from consumer products.

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In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide

Cited by 53 publications

References 21 publications

<p>Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection</p>

<p>Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection</p>

Nanoparticles in Daily Life: Applications, Toxicity and Regulations

In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

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