Multiregional dissemination of KPC-producing Klebsiella pneumoniae ST258/ST512 genotypes in Poland, 2010–14

Baraniak, Anna; Izdebski, Radosław; Żabicka, Dorota; Bojarska, Katarzyna; Górska, Sandra; Literacka, Elżbieta; Fiett, Janusz; Hryniewicz, Waleria; Gniadkowski, Marek

doi:10.1093/jac/dkx054

Cited by 21 publications

(7 citation statements)

References 67 publications

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“…K. pneumoniae ST258 isolates containing bla KPC in the bacterial chromosome now have been reported by several different groups and may be more common than originally expected (216,217). Sequence types other than ST258 have also been reported, with major representatives including ST11, the predominant type in China and Taiwan, and ST25, ST147, and ST512 in the Americas and Europe (210,213,(217)(218)(219)(220).…”

Section: Carbapenemase-producing Bacteriamentioning

confidence: 92%

“…These isolates were divided between two clades, with both bla KPC-2 and bla KPC-3 located on IncFII K2-like plasmids; ST258 clade I was more frequently associated with bla KPC-2, whereas clade II was more frequently associated with bla KPC-3 (210). Large outbreaks of KPC-producing K. pneumoniae in Poland and Colombia have also been attributed to strains belonging to ST258, with bla KPC often found inside the Tn4401 transposon (213,214). In an extensive European genomic study of carbapenem-resistant K. pneumoniae from 244 hospitals in 32 countries, ST258/512 K. pneumoniae, originating in the United States, was identified as the likely progenitor of the large majority of European carbapenemase-producing isolates (215).…”

Section: Carbapenemase-producing Bacteriamentioning

confidence: 99%

“…In addition to K. pneumoniae, KPC-type ␤-lactamases were also found in E. aerogenes, E. coli, K. oxytoca, C. freundii, and several other species (199). Outside the United States, recent outbreaks of KPCproducing K. pneumoniae have been reported in Brazil, China, Ecuador, Germany, Greece, South Korea, Poland, Portugal, Switzerland, and Taiwan (213,(218)(219)(220)(225)(226)(227)(228)(229)(230)(231)(232). A widely publicized outbreak of KPC-producing K. pneumoniae in the United States was documented in a clinical trial unit at the National Institutes of Health, resulting in the death of 11 of 18 infected patients as a result of infection-associated causes (233).…”

Section: Carbapenemase-producing Bacteriamentioning

confidence: 99%

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Epidemiology of β-Lactamase-Producing Pathogens

Bush

Bradford²

2020

Clin Microbiol Rev

522

440

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SUMMARY β-Lactam antibiotics have been widely used as therapeutic agents for the past 70 years, resulting in emergence of an abundance of β-lactam-inactivating β-lactamases. Although penicillinases in Staphylococcus aureus challenged the initial uses of penicillin, β-lactamases are most important in Gram-negative bacteria, particularly in enteric and nonfermentative pathogens, where collectively they confer resistance to all β-lactam-containing antibiotics. Critical β-lactamases are those enzymes whose genes are encoded on mobile elements that are transferable among species. Major β-lactamase families include plasmid-mediated extended-spectrum β-lactamases (ESBLs), AmpC cephalosporinases, and carbapenemases now appearing globally, with geographic preferences for specific variants. CTX-M enzymes include the most common ESBLs that are prevalent in all areas of the world. In contrast, KPC serine carbapenemases are present more frequently in the Americas, the Mediterranean countries, and China, whereas NDM metallo-β-lactamases are more prevalent in the Indian subcontinent and Eastern Europe. As selective pressure from β-lactam use continues, multiple β-lactamases per organism are increasingly common, including pathogens carrying three different carbapenemase genes. These organisms may be spread throughout health care facilities as well as in the community, warranting close attention to increased infection control measures and stewardship of the β-lactam-containing drugs in an effort to control selection of even more deleterious pathogens.

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Section: Carbapenemase-producing Bacteriamentioning

confidence: 92%

Section: Carbapenemase-producing Bacteriamentioning

confidence: 99%

Section: Carbapenemase-producing Bacteriamentioning

confidence: 99%

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Epidemiology of β-Lactamase-Producing Pathogens

Bush

Bradford²

2020

Clin Microbiol Rev

522

440

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“…Although there are no previous data on IS disruptions within the O-locus, in general, these elements are common in K. pneumoniae genomes [ 30 ]. The hyperepidemic clone ST258, characterized by the notorious production of KPC-type carbapenemases and extensive drug resistance, has more ISs than an average K. pneumoniae isolate of another ST [ 30 , 31 ]. Several IS types are especially frequent in ST258, such as IS Kpn26 [ 30 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Insertion Sequences on O-Serotype Phenotype and Its O-Locus-Based Prediction in Klebsiella pneumoniae O2 and O1

Artyszuk

Izdebski

Maciejewska

et al. 2020

IJMS

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Klebsiella pneumoniae is a nosocomial pathogen, pointed out by the World Helth Organisation (WHO) as “critical” regarding the highly limited options of treatment. Lipopolysaccharide (LPS, O-antigen) and capsular polysaccharide (K-antigen) are its virulence factors and surface antigens, determining O- and K-serotypes and encoded by O- or K-loci. They are promising targets for antibody-based therapies (vaccines and passive immunization) as an alternative to antibiotics. To make such immunotherapy effective, knowledge about O/K-antigen structures, drift, and distribution among clinical isolates is needed. At present, the structural analysis of O-antigens is efficiently supported by bioinformatics. O- and K-loci-based genotyping by polymerase chain reaction (PCR) or whole genome sequencing WGS has been proposed as a diagnostic tool, including the Kaptive tool available in the public domain. We analyzed discrepancies for O2 serotyping between Kaptive-based predictions (O2 variant 2 serotype) and the actual phenotype (O2 variant 1) for two K. pneumoniae clinical isolates. Identified length discrepancies from the reference O-locus resulted from insertion sequences (ISs) within rfb regions of the O-loci. In silico analysis of 8130 O1 and O2 genomes available in public databases indicated a broader distribution of ISs in rfbs that may influence the actual O-antigen structure. Our results show that current high-throughput genotyping algorithms need to be further refined to consider the effects of ISs on the LPS O-serotype.

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“…Several outbreaks of K. pneumoniae infections have been reported in hospital settings from different countries, including China, Israel, Poland, Italy, Colombia, and the United States (Kaye and Dhar, 2016;Ocampo et al, 2016;Baraniak et al, 2017;Krapp et al, 2018;Sotgiu et al, 2018;Liu and Guo, 2019). Reportedly, K. pneumoniae accounts for ∼12% of all hospital-acquired pneumonia in the world (Ashurst and Dawson, 2019).…”

Section: Introductionmentioning

confidence: 99%

Novel Target Exploration from Hypothetical Proteins of Klebsiella pneumoniae MGH 78578 Reveals a Protein Involved in Host-Pathogen Interaction

Pranavathiyani

Prava

Rajeev

et al. 2020

Front. Cell. Infect. Microbiol.

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The opportunistic pathogen Klebsiella pneumoniae is a causative agent of several hospital-acquired infections. It has become resistant to a wide range of currently available antibiotics, leading to high mortality rates among patients; this has further led to a demand for novel therapeutic intervention to treat such infections. Using a series of in silico analyses, the present study aims to explore novel drug/vaccine candidates from the hypothetical proteins of K. pneumoniae. A total of 540 proteins were found to be hypothetical in this organism. Analysis of these 540 hypothetical proteins revealed 30 pathogen-specific proteins essential for pathogen survival. A motifs/domain family analysis, similarity search against known proteins, gene ontology, and protein-protein interaction analysis of the shortlisted 30 proteins led to functional assignment for 17 proteins. They were mainly cataloged as enzymes, lipoproteins, stress-induced proteins, transporters, and other proteins (viz., two-component proteins, skeletal proteins and toxins). Among the annotated proteins, 16 proteins, located in the cytoplasm, periplasm, and inner membrane, were considered as potential drug targets, and one extracellular protein was considered as a vaccine candidate. A druggability analysis indicated that the identified 17 drug/vaccine candidates were "novel". Furthermore, a host-pathogen interaction analysis of these identified target candidates revealed a betaine/carnitine/choline transporters (BCCT) family protein showing interactions with five host proteins. Structure prediction and validation were carried out for this protein, which could aid in structure-based inhibitor design.

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Multiregional dissemination of KPC-producing Klebsiella pneumoniae ST258/ST512 genotypes in Poland, 2010–14

Abstract: The study showed remarkable changes in the KPC epidemiology in Poland in 2010-14, which, following the localized regional spread in the early phase, has converted into multiregional dissemination.

Cited by 21 publications

References 67 publications

Epidemiology of β-Lactamase-Producing Pathogens

Epidemiology of β-Lactamase-Producing Pathogens

The Impact of Insertion Sequences on O-Serotype Phenotype and Its O-Locus-Based Prediction in Klebsiella pneumoniae O2 and O1

Novel Target Exploration from Hypothetical Proteins of Klebsiella pneumoniae MGH 78578 Reveals a Protein Involved in Host-Pathogen Interaction

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