In vitro cytotoxicity evaluation of resveratrol-loaded nanoparticles: Focus on the challenges of in vitro methodologies

Increased Water-Solubility and Maintained Antioxidant Power of Resveratrol by Its Encapsulation in Vitamin E TPGS Micelles: A Potential Nutritional Supplement for Chronic Liver Disease

“…Our test is a DNA-based assay that has proved to be among the most sensitive cell health indicators. Our results are in concordance with those obtained with NR, which demonstrated a significant reduction of cell viability after 24 h with 25 µm of RES [ 51 ]. The toxicity data agree with stability studies and release studies.…”

“…It is noteworthy that only RES-TPGSs never exerted any effect on cell viability, whereas both RES and (to a great extent) TPGS triggered some toxic actions at higher doses after 24 h. In a recent study, RES induced a decrease in a concentration-dependent manner of HaCaT viability, detected by comparing different assays: MTT, neutral red uptake (NR), Alamar Blue ® , and trypan blue. The IC 50 values for RES varied between 174 and 412 µm, demonstrating a significant variability among the different tests employed [ 51 ]. This could depend on the different cell-death mechanism evaluated in each assay: metabolic activity (Alamar Blue ® , MTT), cell membrane damage (trypan blue), and lysosomal integrity (NR).…”

Microencapsulation of α-tocopherol with zein and β-cyclodextrin using spray drying for colour stability and shelf-life improvement of fruit beverages

“…Aer 8 days of culture, cells were incubated with different freshly prepared dilutions from homogeneous stock suspensions (10 mg mL À1 ) of each particulate system, as described by several authors. 35,36 The range of concentrations tested for both samples was 0.04-10 mg mL À1 . Control wells were performed by incubating the cells with the culture medium.…”

Nanostructured Lipid Carriers for the Formulation of Topical Anti-Inflammatory Nanomedicines Based on Natural Substances

“…The enhanced permeation happens because NLC cause an occlusion effect through the formation of a film on the skin ( Figure 5 ) [ 99 , 100 ]. The occlusive effect reduces transepidermal water loss, improving hydration of the skin, and increasing drug penetration [ 18 , 27 , 43 , 101 , 102 , 103 , 104 ]. This effect is conferred by the small size and strong adhesive properties of these particles [ 43 , 99 , 102 , 104 , 105 ].…”

“…The occlusive effect reduces transepidermal water loss, improving hydration of the skin, and increasing drug penetration [ 18 , 27 , 43 , 101 , 102 , 103 , 104 ]. This effect is conferred by the small size and strong adhesive properties of these particles [ 43 , 99 , 102 , 104 , 105 ]. In addition, NLC components, such as lipids and surfactants, can also act as permeation enhancers by interacting with and disorganizing SC lipids, which facilitates permeation of the molecules to the deeper layers of the epidermis [ 20 , 43 ].…”