Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression

Chen, Xingxiang; Che, Chaoping; Korolchuk, Viktor I.; Gan, Fang; Chen, Pan; Huang, Kehe

doi:10.1021/acs.jafc.6b05308

Cited by 33 publications

(11 citation statements)

References 55 publications

(106 reference statements)

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“…Surely, it would be interesting to use realistic AFB1 concentrations; however, treating cells with an amount of AFB1 slightly higher may help to highlight the molecular mechanisms involved in the toxicity. Notably, the increasing AFB1 concentrations chosen in the present study were in accordance to those selected in previous cytotoxic experiments conducted in human and chicken hepatocytes, as well as in similar transcriptomic studies aimed at unveiling AFB1 mechanism of toxicity in chicken hepatocellular carcinoma (LMH) and HepG2 cell lines [29][30][31][32][33]. Overall, these data confirm AFB1 is cytotoxic to bovine hepatocytes, as previously reported [24].…”

Section: Afb1 Cytotoxicity and Mechanism Of Cell Deathsupporting

confidence: 89%

Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2

Iori

Pauletto

Bassan

et al. 2022

Toxins

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Aflatoxin B1 (AFB1) is a food contaminant metabolized mostly in the liver and leading to hepatic damage. Livestock species are differently susceptible to AFB1, but the underlying mechanisms of toxicity have not yet been fully investigated, especially in ruminants. Thus, the aim of the present study was to better characterize the molecular mechanism by which AFB1 exerts hepatotoxicity in cattle. The bovine fetal hepatocyte cell line (BFH12) was exposed for 48 h to three different AFB1 concentrations (0.9 µM, 1.8 µM and 3.6 µM). Whole-transcriptomic changes were measured by RNA-seq analysis, showing significant differences in the expression of genes mainly involved in inflammatory response, oxidative stress, drug metabolism, apoptosis and cancer. As a confirmatory step, post-translational investigations on genes of interest were implemented. Cell death associated with necrosis rather than apoptosis events was noted. As far as the toxicity mechanism is concerned, a molecular pathway linking inflammatory response and oxidative stress was postulated. Toll-Like Receptor 2 (TLR2) activation, consequent to AFB1 exposure, triggers an intracellular signaling cascade involving a kinase (p38β MAPK), which in turn allows the nuclear translocation of the activator protein-1 (AP-1) and NF-κB, finally leading to the release of pro-inflammatory cytokines. Furthermore, a p38β MAPK negative role in cytoprotective genes regulation was postulated. Overall, our investigations improved the actual knowledge on the molecular effects of this worldwide relevant natural toxin in cattle.

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Section: Afb1 Cytotoxicity and Mechanism Of Cell Deathsupporting

confidence: 89%

Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2

Iori

Pauletto

Bassan

et al. 2022

Toxins

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“…SelW is a selenoprotein with an essential role in maintaining normal liver function. According to this recent report, SeMet suppressed the expression of CYP450 1A5 enzyme which activates AFB1 to transform to toxic AFBO form; while SelW knockdown with SelW-specific siRNA significantly increased mRNA and protein levels of CYP450 1A5, thus indicating the protective role of SeMet [ 102 ].…”

Section: Selenium-mediated Afb1 Toxicity Control and Protective Mementioning

confidence: 99%

Protective and Detoxifying Effects Conferred by Dietary Selenium and Curcumin against AFB1-Mediated Toxicity in Livestock: A Review

Limaye

Chou

et al. 2018

Toxins

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Aflatoxin B1 (AFB1), among other aflatoxins of the aflatoxin family, is the most carcinogenic and hazardous mycotoxin to animals and human beings with very high potency leading to aflatoxicosis. Selenium is an essential trace mineral possessing powerful antioxidant functions. Selenium is widely reported as an effective antioxidant against aflatoxicosis. By preventing oxidative liver damage, suppressing pro-apoptotic proteins and improving immune status in AFB1 affected animals; selenium confers specific protection against AFB1 toxicity. Meticulous supplementation of animal feed by elemental selenium in the organic and inorganic forms has proven to be effective to ameliorate AFB1 toxicity. Curcumin is another dietary agent of importance in tackling aflatoxicosis. Curcumin is one of the major active ingredients in the tubers of a spice Curcuma longa L., a widely reported antioxidant, anticarcinogenic agent with reported protective potential against aflatoxin-mediated liver damage. Curcumin restricts the aflatoxigenic potential of Aspergillus flavus. Curcumin inhibits cytochrome P450 isoenzymes, particularly CYP2A6 isoform; thereby reducing the formation of AFB1-8, 9-epoxide and other toxic metabolites causing aflatoxicosis. In this review, we have briefly reviewed important aflatoxicosis symptoms among animals. With the main focus on curcumin and selenium, we have reviewed their underlying protective mechanisms in different animals along with their extraction and production methods for feed applications.Keywords: aflatoxin B1; anti-AFB1 dietary supplement; curcumin; selenium; antioxidant status Key contribution: We have reviewed protective roles of selenium and curcumin in specific relation to tacking AFB1 toxicity in the livestock along with their reported underlying mechanisms for the potential feed applications catering across a broad host range.

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“…Deoxynivalenol, zearalenone, nivalenol, ochratoxin A (OTA), aflatoxin B1 (AFB1), and fumonisin B1 (FB1) are some of the most predominant mycotoxins [75]. Among them, AFB1 is listed as group I carcinogens by the International Agency for Research on Cancer [76] and is claimed as the most toxic mycotoxin due to its capacity to bind with the DNA of cells increasing the risk of liver cancer in human beings [77]. The US Food and Drug Administration (FDA) has set the limited level of AFB1 in corn and peanut feeds for finishing beef cattle at 300 ng•mL −1 [78].…”

Section: Toxinsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression

Cited by 33 publications

References 55 publications

Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2

Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2

Protective and Detoxifying Effects Conferred by Dietary Selenium and Curcumin against AFB1-Mediated Toxicity in Livestock: A Review

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

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