A comparison of intact and degraded desmin in cooked and uncooked pork longissimus thoracis and their relationship to pork quality

Richardson, Elaine; Bohrer, Benjamin M.; Arkfeld, E. K.; Boler, D. D.; Dilger, A. C.

doi:10.1016/j.meatsci.2017.02.024

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…We speculated that the miR-10 family is tightly regulated by muscle fiber transformation. The target genes of the miR-10 family in the ceRNA network included DES [ 20 ], MYBPH [ 21 ] and SMAD3 [ 22 ]. To verify this hypothesis, we constructed MYBPH wild-type (WT) plasmids and mutantion (MUT) plasmids for co-transfection with miR-10a-5p mimics, and dual luciferase reporter genes determined that miR-10a-5p and MYBPH had strong binding ability (Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Li,

Wang,

Sun

et al. 2024

BMC Genomics

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Background Skeletal muscle is composed of muscle fibers with different physiological characteristics, which plays an important role in regulating skeletal muscle metabolism, movement and body homeostasis. The type of skeletal muscle fiber directly affects meat quality. However, the transcriptome and gene interactions between different types of muscle fibers are not well understood. Results In this paper, we selected 180-days-old Large White pigs and found that longissimus dorsi (LD) muscle was dominated by fast-fermenting myofibrils and soleus (SOL) muscle was dominated by slow-oxidizing myofibrils by frozen sections and related mRNA and protein assays. Here, we selected LD muscle and SOL muscle for transcriptomic sequencing, and identified 312 differentially expressed mRNA (DEmRs), 30 differentially expressed miRNA (DEmiRs), 183 differentially expressed lncRNA (DElRs), and 3417 differentially expressed circRNA (DEcRs). The ceRNA network included ssc-miR-378, ssc-miR-378b-3p, ssc-miR-24-3p, XR_308817, XR_308823, SMIM8, MAVS and FOS as multiple core nodes that play important roles in muscle development. Moreover, we found that different members of the miR-10 family expressed differently in oxidized and glycolytic muscle fibers, among which miR-10a-5p was highly expressed in glycolytic muscle fibers (LD) and could target MYBPH gene mRNA. Therefore, we speculate that miR-10a-5p may be involved in the transformation of muscle fiber types by targeting the MYHBP gene. In addition, PPI analysis of differentially expressed mRNA genes showed that ACTC1, ACTG2 and ACTN2 gene had the highest node degree, suggesting that this gene may play a key role in the regulatory network of muscle fiber type determination. Conclusions We can conclude that these genes play a key role in regulating muscle fiber type transformation. Our study provides transcriptomic profiles and ceRNA interaction networks for different muscle fiber types in pigs, providing reference for the transformation of pig muscle fiber types and the improvement of meat quality.

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Section: Resultsmentioning

confidence: 99%

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Li,

Wang,

Sun

et al. 2024

BMC Genomics

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“…Desmin is a substrate of calpain, which is important for cell integrity and muscle function, and was of great significance in the study of meat texture. Richardson demonstrated that reducing noggin degradation generally improves the water holding capacity of meat products [48]. Table 2 shows that desmin was closely related to the hardness of grouper fillet, possibly due to desmin degradation during storage.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Proteins Associated with Quality Deterioration of Grouper Fillets Based on TMT Quantitative Proteomics during Refrigerated Storage

Zhang

Xie

2019

Molecules

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A TMT (Tandem Mass Tag)-based strategy was applied to elucidate proteins that change in proteomes of grouper fillets during refrigerated storage. In addition, quality analyses on pH, centrifugal loss, color (L *, a *, b *) and texture (hardness, chewiness, and gumminess) for grouper fillets were performed. A total of 64 differentially significant expressed proteins (DSEPs) were found in the results in the Day 0 vs. Day 6 group comparison and the Day 0 vs. Day 12 group comparison. It is worth mentioning that more proteome changes were found in the Day 0 vs. Day 12 comparisons. Bioinformatics was utilized to analyze the DSEP. UniProt Knowledgebase (UniProtKB), Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein interaction network analysis were adopted. All DSEPs were classified into seven areas by function: binding proteins, calcium handling, enzymes, heat shock protein, protein turnover, structural proteins and miscellaneous. The numbers of proteins that correlated closely with pH, centrifugal loss, color (L *, a *, b *) and texture (hardness, chewiness, and gumminess) were 4, 3, 6 and 8, respectively.

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Degradation and evaluation of myofibril proteins induced by endogenous protease in aquatic products during storage: a review

Qian

Chu

2023

Food Sci Biotechnol

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A comparison of intact and degraded desmin in cooked and uncooked pork longissimus thoracis and their relationship to pork quality

Cited by 7 publications

References 17 publications

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Analysis of Proteins Associated with Quality Deterioration of Grouper Fillets Based on TMT Quantitative Proteomics during Refrigerated Storage

Degradation and evaluation of myofibril proteins induced by endogenous protease in aquatic products during storage: a review

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