Susceptibility to disinfectants in antimicrobial-resistant and -susceptible isolates of Escherichia coli, Enterococcus faecalis and Enterococcus faecium from poultry-ESBL/AmpC-phenotype of E. coli is not associated with resistance to a quaternary ammonium

Wieland, N.; Boss, J.; Lettmann, S.; Fritz, Blaine Gabriel; Schwaiger, Karin; Bauer, Johann; Hölzel, Christina

doi:10.1111/jam.13440

Cited by 34 publications

(26 citation statements)

References 37 publications

(43 reference statements)

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“…The high reproducibility points towards the reliable description of susceptibility patterns for cationic biocides by applying a test system based on vacuum dried plates. Overall, MIC and MBC values of BAC, DDAC, and CHX were in accordance with previously reported data on E. faecium, which were generated by broth microdilution according to ISO 20776-1 [13,21], modified broth microdilution and subsequent macrodilution [22,23], or agar dilution [14]. Interestingly, compared to data published by Morrissey et al [21], MIC values of CHX were quite low (2-4 mg/L vs. 16 mg/L) in our subpopulation of E. faecium isolates, which might display geographic variability.…”

Section: Cationic Biocide Susceptibility Profiles Of Vancomycin Resissupporting

confidence: 89%

Evaluation of a Newly Developed Vacuum Dried Microtiter Plate for Rapid Biocide Susceptibility Testing of Clinical Enterococcus faecium Isolates

et al. 2020

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We investigated the suitability of a newly developed biocide susceptibility test system based on microtiter plates containing vacuum dried biocides as a fast and reliable screening method. The evaluated substances included the cationic biocides benzalkonium chloride (BAC), chlorhexidine dihydrochloride (CHX), cetylpyridinium chloride, didecyldimethylammonium chloride, and octenidine dihydrochloride. Testing a selection of Escherichia coli and enterococci, the biocide microtiter plates provided results comparable to those obtained from broth microdilution according to ISO 20776-1. Broad MIC ranges allowed for testing gram-positive and gram-negative species with the same plate design. In the second part of our study, we applied the established method to analyze the susceptibility of 90 clinical Enterococcus faecium isolates from a German university hospital, as previous studies have indicated a link between reduced susceptibility to substances such as CHX and BAC and vancomycin resistance. We therefore determined MIC and minimum bactericidal concentrations (MBC) for 48 non-clonal vancomycin susceptible and 42 non-clonal vancomycin resistant isolates, but MIC95 and MBC95 were quite similar in both groups. Our easy to handle and ready to use test system enables the routine surveillance of bacterial tolerance towards disinfectants in hospitals. As a result, hygiene measures can be adapted and nosocomial infections controlled despite increasing prevalence of antibiotic-resistant bacteria.

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Section: Cationic Biocide Susceptibility Profiles Of Vancomycin Resissupporting

confidence: 89%

Evaluation of a Newly Developed Vacuum Dried Microtiter Plate for Rapid Biocide Susceptibility Testing of Clinical Enterococcus faecium Isolates

et al. 2020

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“…For example, in 153 E. coli blood culture isolates a higher MIC of didecyldimethylammonium chloride was associated with a decreased susceptibility to cotrimoxazole [21]. In E. coli didecyldimethylammonium chloride-MICs were positively correlated with MICs of piperacillin and sulphamethoxazole-trimethoprim [60]. However exposure of A. baumannii , C. sakazakii , E. coli , P. aeruginosa and P. putida to increasing didecyldimethylammonium chloride concentrations over 14 passages of four days each did not result in antibiotic resistance [61].…”

Section: Didecyldimethylammonium Chloridementioning

confidence: 99%

Biocidal Agents Used for Disinfection Can Enhance Antibiotic Resistance in Gram-Negative Species

2018

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Biocidal agents used for disinfection are usually not suspected to enhance cross-resistance to antibiotics. The aim of this review was therefore to evaluate the effect of 13 biocidal agents at sublethal concentrations on antibiotic resistance in Gram-negative species. A medline search was performed for each biocidal agent on antibiotic tolerance, antibiotic resistance, horizontal gene transfer, and efflux pump. In cells adapted to benzalkonium chloride a new resistance was most frequently found to ampicillin (eight species), cefotaxime (six species), and sulfamethoxazole (three species), some of them with relevance for healthcare-associated infections such as Enterobacter cloacae or Escherichia coli. With chlorhexidine a new resistance was often found to ceftazidime, sulfamethoxazole and imipenem (eight species each) as well as cefotaxime and tetracycline (seven species each). Cross-resistance to antibiotics was also found with triclosan, octenidine, sodium hypochlorite, and didecyldimethylammonium chloride. No cross-resistance to antibiotics has been described after low level exposure to ethanol, propanol, peracetic acid, polyhexanide, povidone iodine, glutaraldehyde, and hydrogen peroxide. Taking into account that some biocidal agents used in disinfectants have no health benefit (e.g., in alcohol-based hand rubs) but may cause antibiotic resistance it is obvious to prefer products without them.

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“…Furthermore, contradictory results have been found regarding the possible association between the reduced susceptibility to disinfectants and antibiotic resistance in field bacteria [8][9][10][11]. Moreover, few studies were performed on the use of disinfectants and the relation to antimicrobial resistance under practical conditions [12].…”

Section: Introductionmentioning

confidence: 99%

Repeated disinfectant use in broiler houses and pig nursery units does not affect disinfectant and antibiotic susceptibility in Escherichia coli field isolates

et al. 2020

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Background: Disinfectants are frequently used in animal production to reduce or eliminate the load of infectious agents and parasites in buildings and equipment associated with the housing or transportation of animals. There are growing concerns that the use of disinfectants would select for resistance to antibiotics and disinfectants. The aim of this study was to determine the effect of repeated use of different disinfectants on the disinfectant and antibiotic susceptibility under practical conditions in a broiler and pig pilot farm. Therefore, the susceptibility of Escherichia coli (E. coli) to 14 antibiotics and 4 disinfectants was monitored over a one-year period. Results: High (20-50%) to very high (> 50%) resistance levels for ampicillin, sulfamethoxazole, trimethoprim and tetracycline were observed in both animal production types. Disinfectant susceptibility did not change over time and did not depend on the used disinfection product. Compared to in-use concentrations of formaldehyde, benzalkoniumchloride and a peracetic acid-hydrogen peroxide formulation, all E. coli strains remained susceptible indicating that the use of disinfectants did not select for disinfectant resistance. Moreover, no association could be found between the use of disinfectants and antibiotic resistance. Conclusions: These findings suggest that repeated use of disinfectants in agricultural environments does not select for antibiotic resistance nor does it reduce disinfectant susceptibility.

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Susceptibility to disinfectants in antimicrobial-resistant and -susceptible isolates of Escherichia coli, Enterococcus faecalis and Enterococcus faecium from poultry-ESBL/AmpC-phenotype of E. coli is not associated with resistance to a quaternary ammonium

Cited by 34 publications

References 37 publications

Evaluation of a Newly Developed Vacuum Dried Microtiter Plate for Rapid Biocide Susceptibility Testing of Clinical Enterococcus faecium Isolates

Evaluation of a Newly Developed Vacuum Dried Microtiter Plate for Rapid Biocide Susceptibility Testing of Clinical Enterococcus faecium Isolates

Biocidal Agents Used for Disinfection Can Enhance Antibiotic Resistance in Gram-Negative Species

Repeated disinfectant use in broiler houses and pig nursery units does not affect disinfectant and antibiotic susceptibility in Escherichia coli field isolates

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