Acute resistance exercise reduces increased gene expression in muscle atrophy of ovariectomised arthritic rats

Abstract

ObjectiveWe studied the effect of resistance exercise (RE) on mRNA levels of atrogin-1, MuRF-1, and myostatin in the gastrocnemius muscle of arthritic rats after loss of ovarian function (LOF).Material and methodsThirty female Wistar rats (nine weeks old, 195.3 ±17.4 grams) were randomly allocated into five groups: control group (CT-Sham; n = 6); group with rheumatoid arthritis (RA; n = 6); group with rheumatoid arthritis subjected to RE (RAEX; n = 6); ovariectomy group with rheumatoid arthritis (RAOV; n = 6); and an ovariectomy group with rheumatoid arthritis subjected to RE (RAOVEX; n = 6). After 15 days of intra-articular injections with Met-BSA the animals were subjected to RE and six hours after workout were euthanised.ResultsThe rheumatoid arthritis provoked reduction in the cross-sectional area (CSA) of muscle fibres, but the CSA was lower in the RAOV when compared to the RA groups. Skeletal muscle atrogin-1 mRNA level was increased in arthritic rats (RA and RAOV), but the atrogin-1 level was higher in RAOV group when compared to other arthritic groups. The Muscle MuRF-1 mRNA level was also increased in the RAOV group. The increased atrogin-1 and MuRF-1 mRNA levels were lower in the RAOVEX group than in the RAOV group. The myostatin mRNA level was similar in all groups, except for the RAOVEX group, in which it was lower than the other groups.ConclusionsLOF results in increased loss of skeletal muscle-related ubiquitin ligases (atrogin-1 and MuRF-1). However, the RE reduces the atrogin-1, MuRF-1, and myostatin mRNA levels in muscle of arthritic rats affected by LOF.

Concurrent treatment with ursolic acid and low-intensity treadmill exercise improves muscle atrophy and related outcomes in rats

“…It decreases the expression of MuRF1 and Atrogin-1 and improves the insulin-like growth factor 1 (IGF-1) signaling pathway for muscle hypertrophy. Resistance training also increases muscle hypertrophy through increased mTOR signaling action [ 56 57 ] and decreases muscle atrophy-related gene expression [ 9 58 ]. In this regard, although UA is considered to be an exercise mimetic for muscle atrophy improvement, this study could not confirm the effect of UA alone, which may be similar to the weight loss effect induced through exercise.…”

Effects of ursolic acid on muscle mass and bone microstructure in rats with casting-induced muscle atrophy

“…Skeletal muscle-bone interaction involves crosstalk 19 - 21 . Therefore, resistance exercise for building muscle can be a highly effective treatment to preserve and increase BMD and bone strength 22 , 23 . However, due to the high risk of musculoskeletal injury 24 , resistance exercise has limited applications, especially in the elderly with less physical strength.…”