Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from<i>Sulfolobus acidocaldarius</i>

Engelhardt, Konrad; Pinnapireddy, Shashank Reddy; Baghdan, Elias; Jedelská, Jarmila

doi:10.1155/2017/8047149

Cited by 25 publications

(16 citation statements)

References 27 publications

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“…In addition, the unique molecular structure of TELs is attributed to the presence of cyclopentane rings, methyl side groups and sugar moieties that create extensive hydrogen bond network. The presence of ether bonds gives rise to molecular stability (Engelhardt et al., 2017 ), in addition to the lacking of double bonds which make TELs an epitome candidate for PDT. One of the elementary factors for tumor progression, for instance, growth, invasion and metastasis, is angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

et al. 2018

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Photodynamic therapy (PDT) that involves ergonomically delivered light in the presence of archetypical photosensitizer such as Protoporphyrin IX (PpIX) is a time-honored missile strategy in cancer therapeutics. Yet, the premature release of PpIX is one of the most abundant dilemma encounters the therapeutic outcomes of PDT due to associated toxicity and redistribution to serum proteins. In this study, ultrastable tetraether lipids (TELs) based liposomes were developed. PpIX molecules were identified to reside physically in the monolayer; thereby the inherent π-π stacking that leads to aggregation of PpIX in aqueous milieu was dramatically improved. TEL29.9 mol% and TEL62mol% based liposomes revealed PpIX sustained release diffusion pattern from spherical particles as confirmed by converged fitting to Baker & Lonsdale model. Stability in presence of human serum albumins, a key element for PDT accomplishment was emphasized. The epitome candidates were selected for vascular photodynamic (vPDT) in in-Ovo chick chorioallantoic membrane. Profoundly, TEL62mol% based liposomes proved to be the most effective liposomes that demonstrated localized effect within the irradiated area without eliciting quiescent vasculatures damages. Cellular photodynamic therapy (cPDT) revealed that various radiant exposure doses of 134, 202, 403 or 672 mJ.cm−2 could deliberately modulate the photo-responses of PpIX in TEL-liposomes.

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Section: Introductionmentioning

confidence: 99%

Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

et al. 2018

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“…1,2-distearoyl- sn -glycero-3- phosphatidyl-choline (DSPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidyl-choline (DPPC), were a gift from Lipoid GmbH (Ludwigshafen, Germany). A polar lipid fraction containing tetraether lipids caldarchaeol (GDGT ∼10% of the extract) and calditoglycerocaldarchaeol (GDNT ∼90% of the extract) was extracted from the biomass of S. acidocaldarius (SIT Rosenhof GmbH, Heiligenstadt, Germany) as previously mentioned (Engelhardt et al., 2017). (2-hydroxypropyl)-β-cyclodextrin (HPβCD) and all other chemicals and solvents used were of analytical grade and were purchased from Sigma-Aldrich (Taufkirchen, Germany).…”

Section: Methodsmentioning

confidence: 99%

Photodynamic therapy – hypericin tetraether liposome conjugates and their antitumor and antiangiogenic activity

et al. 2019

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Photodynamic therapy (PDT) is an established noninvasive tumor treatment. The hydrophobic natural occurring pigment hypericin shows a lot of attractive properties for the application in PDT. Hence, the administration to biological systems or patients requires the formulation in drug carriers enabling sufficient bioavailability. Therefore, free hypericin was encapsulated by the thin film hydration method or a hypericin-hydroxypropyl-β-cyclodextrin inclusion complex (Hyp-HPβCD) was incorporated by dehydration-rehydration vesicle method in either conventional or ultra-stable tetraether lipid (TEL) liposomes. The hydrodynamic diameter of the prepared nanoformulations ranged between 127 and 212 nm. These results were confirmed by atomic force microscopy. All liposomes showed a good stability under physiological conditions. TEL liposomes which tend to build more rigid bilayers, generate higher encapsulation efficiencies than their conventional counterparts. Furthermore, the suitability for intravenous application was confirmed by hemocompatibility studies resulting in a hemolytic potential less than 20% and a coagulation time less than 50 sec. The uptake of liposomal hypericin into human ovarian carcinoma cells (SK-OV-3) was confirmed using confocal microscopy and further characterized by pathway studies. It was demonstrated that the lipid composition and intraliposomal hypericin localization influenced the anti-vascular effect in the chorioallantoic membrane (CAM). While hypericin TEL liposomes exhibit substantial destruction of the microvasculature drug-in-cyclodextrin TEL liposomes showed no effect. Nevertheless, both formulations yielded severe photocytotoxicity in SK-OV-3 cells in a therapeutic dosage range. Conclusively, hypericin TEL liposomes would be perfectly suited for anti-vascular targeting while Hyp-HPβCD TEL liposomes could deliver the photosensitizer to the tumor site in a more protected manner.

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“…Due to the TELs in the liposomes, a slower release of the drug to the cancer cell could be achieved [131]. In another study, mixing cholesterol and TEL extracted from S. acidocaldarius resulted in a stable vector for gene delivery used for the transfection of mammalian cells [132]. These applications, including the oral delivery of drugs, are summarized in Table 3.…”

Section: Applicationsmentioning

confidence: 99%

The Cell Membrane of Sulfolobus spp.—Homeoviscous Adaption and Biotechnological Applications

Rastädter

Wurm

Spadiut

et al. 2020

IJMS

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The microbial cell membrane is affected by physicochemical parameters, such as temperature and pH, but also by the specific growth rate of the host organism. Homeoviscous adaption describes the process of maintaining membrane fluidity and permeability throughout these environmental changes. Archaea, and thereby, Sulfolobus spp. exhibit a unique lipid composition of ether lipids, which are altered in regard to the ratio of diether to tetraether lipids, number of cyclopentane rings and type of head groups, as a coping mechanism against environmental changes. The main biotechnological application of the membrane lipids of Sulfolobus spp. are so called archaeosomes. Archaeosomes are liposomes which are fully or partly generated from archaeal lipids and harbor the potential to be used as drug delivery systems for vaccines, proteins, peptides and nucleic acids. This review summarizes the influence of environmental parameters on the cell membrane of Sulfolobus spp. and the biotechnological applications of their membrane lipids.

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Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids fromSulfolobus acidocaldarius

Cited by 25 publications

References 27 publications

Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

Photodynamic therapy – hypericin tetraether liposome conjugates and their antitumor and antiangiogenic activity

The Cell Membrane of Sulfolobus spp.—Homeoviscous Adaption and Biotechnological Applications

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