Comparison between in vitro properties of washed platelet concentrates suspended in M-sol and those in BRS-A, both of which were prepared with an automated cell processor

Comparison of administration of platelet concentrates suspended in M‐sol or BRS‐A for pediatric patients

“…To effectively prevent ATRs with PC transfusion, it is important to sufficiently remove PCs during the preparation of washed PCs . In our manual method of RPC adjustment, it is possible to remove approximately 90%‐95% of plasma proteins in the preparation process, but it is difficult to remove as many plasma proteins (approximately 99%) as obtained from reported automated equipment methods . However, together with evidence from other reports targeting the same disease population of pediatric patients, our manual method of RPC preparation can effectively prevent ATRs, regardless of the type of PAS (M‐sol or BRS‐A).…”

“…Conversely, BRS‐A has the advantage of being easily adjusted by mixing two bicarbonated Ringer solutions with ACD‐A solution . A comparison of washed PC using M‐sol and BRS‐A in vitro has confirmed that the maintenance effect of platelet quality can be achieved at the same level . Washed PCs, using the classical PAS (G‐sol) have been used in Japan for many years, and the manual washing method was considered as a complicated method .…”

48‐hour interruption of agitation: effect on quality of washed PLT suspended in BRS‐A

“…The residual plasma level of this product, which is prepared using an automated cell processor (ACP215, Haemonetics Corp.) is approximately 1%, and PLT properties can be preserved in vitro for at least 7 days. 1,2 Recently, a retrospective multicenter study reported that this product was effective and safe for prevention of recurrent or severe transfusion reactions. 3 PLT products are generally stored with continuous agitation to maintain their quality; however, the interruption of agitation for 24 hours has only a slight impact on the in vitro properties of washed PLTs.…”

“…PCs (3.0 × 10 11 PLTs) were washed on Day 1 after blood collection using the automated closed-system cell processor as described previously (N = 6). 1,2 Washed PLTs, which were rested 1 hour after preparing, were divided equally into control (120 AE 11 mL) and test units (123 AE 12 mL) using polyolefin containers (KBP600FPN, Kawasumi Co., Ltd.) on Day 1. Control units were agitated from Days 1 to 7.…”

“…Both units were stored at 20 to 24 C. in vitro properties were measured as previously described. 2 Statistical analyses of were performed by a 2-way repeated-measures analysis of variance with the Bonferroni post hoc test (Prism 7, Version 7.02, GraphPad Software, Inc.). A p value of less than 0.05 was considered to be significant.…”