Synthetic Biomarker Design by Using Analyte‐Responsive Acetaminophen

Abstract

The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte-responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof-of-concept, we designed hydrogen peroxide (H O )-responsive APAP (HR-APAP) and succeeded in H O detection with cellular and animal experiments. In fact, for blood samples following HR-APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR-APAP] accurately reflects in vivo differences in H O levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.

A detection system using sensing motif-tethered oligodeoxynucleotides for multiplex biomolecular analysis

“…PB-DNA 1 has selectivity for H 2 O 2 over other reactive oxygen species (ROS). Extracellular ROS 18 generated almost no signal derived from NH 2 -DNA 1 (Fig. S3, ESI†).…”