Physiological and comparative genomic analysis of Acidithiobacillus ferrivorans PQ33 provides psychrotolerant fitness evidence for oxidation at low temperature

Ccorahua-Santo, Robert; Eca, Anika; Abanto, Michel; Guerra, Gregory; Ramírez, Pablo

doi:10.1016/j.resmic.2017.01.007

Cited by 21 publications

(24 citation statements)

References 45 publications

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“…A. ferrivorans is considered the only Acidithiobacillus genus member capable of growing at low temperatures, being the first reported acidophilic psychrotolerant [ 21 , 30 ]. In addition to the ACH strain, only four other strains have been described in the literature: SS3 (Russia), CF27 (USA), YL15 (China), and PQ33 (Peru) [ 21 , 22 , 23 , 24 , 25 ]. The genomes of all five A. ferrivorans strains (available in GenBank: Table S3 ) were searched and compared for the presence of copper resistance genes.…”

Section: Resultsmentioning

confidence: 99%

“…Among the Acidithiobacillus genus, the A. ferrivorans species was initially described as the unique psychrotolerant member, characterized by its ability to oxidize ferrous iron and sulfur, as well as to oxidize inorganic sulfur compounds and sulphide minerals [21]. To date, only five strains (SS3, CF27, ACH, PQ33, and YL15) have been described for this species, isolated from several countries around the world (Russia, USA, China, Peru, and Chile) [21][22][23][24][25]. However, A. ferrivorans' ability to tolerate high heavy metal concentrations remains mostly unexplored, mainly because its psychrotolerant capacity draws greater research interest.…”

Section: Introductionmentioning

confidence: 99%

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Determinants of Copper Resistance in Acidithiobacillus Ferrivorans ACH Isolated from the Chilean Altiplano

Barahona

Castro‐Severyn

Dorador

et al. 2020

Genes

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The use of microorganisms in mining processes is a technology widely employed around the world. Leaching bacteria are characterized by having resistance mechanisms for several metals found in their acidic environments, some of which have been partially described in the Acidithiobacillus genus (mainly on ferrooxidans species). However, the response to copper has not been studied in the psychrotolerant Acidithiobacillus ferrivorans strains. Therefore, we propose to elucidate the response mechanisms of A. ferrivorans ACH to high copper concentrations (0–800 mM), describing its genetic repertoire and transcriptional regulation. Our results show that A. ferrivorans ACH can grow in up to 400 mM of copper. Moreover, we found the presence of several copper-related makers, belonging to cop and cus systems, as well as rusticyanins and periplasmatic acop protein in the genome. Interestingly, the ACH strain is the only one in which we find three copies of copB and copZ genes. Moreover, transcriptional expression showed an up-regulation response (acop, copZ, cusA, rusA, and rusB) to high copper concentrations. Finally, our results support the important role of these genes in A. ferrivorans copper stress resistance, promoting the use of the ACH strain in industrial leaching under low temperatures, which could decrease the activation times of oxidation processes and the energy costs.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determinants of Copper Resistance in Acidithiobacillus Ferrivorans ACH Isolated from the Chilean Altiplano

Barahona

Castro‐Severyn

Dorador

et al. 2020

Genes

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“…Psychrotolerant bacterium A. ferrivorans has been successfully characterized due to its ability to oxidize Fe 2+ more efficiently than A. ferrooxidans at 5 °C (Kupka et al 2007;Escobar et al 2010;Hallberg et al 2010;Ccorahua-Santo et al 2017). Compared to other Acidithiobacillus sp.…”

Section: Introductionmentioning

confidence: 99%

“…Compared to other Acidithiobacillus sp. strains, A. ferrivorans grows more quickly at 5 °C (t d >50 h) in a standard 9K medium (33.3% Fe 2+ ) and in the presence of other substrates such as sulphides (Ccorahua-Santo et al 2017) and pyrite (Barahona et al 2014). Moreover, the microorganism's capacity to fix atmospheric carbon dioxide and its tolerance to heavy metals makes it the best candidate for industrial-scale bioleaching in extremely hostile environments, even at temperatures below 10°C.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we reported the strain PQ33 of A. ferrivorans, which performs better at oxidizing ferrous iron at pH 1.6 and copper sulphide at low temperatures than other A. ferrivorans strains: A. ferrivorans SS3 (Kupka et al 2007;Liljeqvist et al 2011), CF27 (Talla et al 2014) and ACH (Barahona et al 2014). Additionally, subsequent genome and gene expression analyses have determined the principal traits of evolutionary fitness in A. ferrivorans for adaptation to low temperatures and oxidative capacity (Liljeqvist et al 2015;Christel et al 2016;Ccorahua-Santo et al 2017). However, there is only one report of other genomic traits related to elements outside of the chromosomal genome (Tran et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Comparative genomic analysis of two novel plasmids from Acidithiobacillus ferrivorans strain PQ33

Ccorahua

Eca

Ramírez³

et al. 2021

Rev peru biol

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Acidithiobacillus ferrivorans is a psychrotolerant acidophile capable of growing and oxidizing ferrous and sulphide substrates at low temperatures. To date, six genomes of this organism have been characterized; however, evidence of a plasmid in this species has been reported only once, whereby there is no conclusive role of the plasmids in the species. Herein, two novel plasmids of A. ferrivorans PQ33 were molecularly characterized and compared at a genomic scale. The genomes of two plasmids (12 kbp and 10 kbp) from A. ferrivorans PQ33 (NZ_LVZL01000000) were sequenced and annotated. The plasmids, named pAfPQ33-1 (NZ_CP021414.1) and pAfPQ33-2 (NZ_CP021415.1), presented 9 CDS and 13 CDS, respectively. In silico analysis showed proteins involved in conjugation (TraD, MobA, Eep and XerD), toxin-antitoxin systems (HicA and HicB), replication (RepA and DNA binding protein), transcription regulation (CopG), chaperone DnaJ, and a virulence gene (vapD). Furthermore, the plasmids contain sequences similar to phosphate-selective porins O and P and a diguanylate cyclase-phosphodiesterase protein. The presence of these genes suggests the possibility of horizontal transfer, a regulatory system of plasmid maintenance, and adhesion to substrates for A. ferrivorans species and PQ33. This is the first report of plasmids in this strain.

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Microbial Adaptations Under Low Temperature

Singh¹,

Thapa²,

Pandiyan³

et al. 2021

Survival Strategies in Cold-Adapted Microorganisms

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Physiological and comparative genomic analysis of Acidithiobacillus ferrivorans PQ33 provides psychrotolerant fitness evidence for oxidation at low temperature

Cited by 21 publications

References 45 publications

Determinants of Copper Resistance in Acidithiobacillus Ferrivorans ACH Isolated from the Chilean Altiplano

Determinants of Copper Resistance in Acidithiobacillus Ferrivorans ACH Isolated from the Chilean Altiplano

Comparative genomic analysis of two novel plasmids from Acidithiobacillus ferrivorans strain PQ33

Microbial Adaptations Under Low Temperature

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