Molecular cloning and expression analysis of toll-like receptor genes (TLR7, TLR8 and TLR9) of golden pompano (Trachinotus ovatus)

Wei, Youchuan; Shu, Hu; Sun, Baobao; Zhang, Qihuan; Qiao, Guo; Shao, Rong; Huang, Guoqiang; Qi, Zhitao

doi:10.1016/j.fsi.2017.02.026

Cited by 31 publications

(6 citation statements)

References 39 publications

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“…TLR7 and TLR8 in golden pompano were reported to exhibit the typical TLR domain architecture, including a TIR domain located in the cytoplasmic region ( 64 ). When TLRs recognize PAMPs, the TLR conformation changes, and then adaptor molecules, including Myd88, are recruited ( 65 , 66 ).…”

Section: Discussionmentioning

confidence: 99%

R848 Is Involved in the Antibacterial Immune Response of Golden Pompano (Trachinotus ovatus) Through TLR7/8-MyD88-NF-κB-Signaling Pathway

et al. 2021

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R848 is an imidazoquinoline compound that is a specific activator of toll-like receptor (TLR) 7/8 and is often used in immunological research in mammals and teleosts. However, the immune responses initiated by R848 through the TLR7/8 pathway in response to bacterial infection remain largely unexplored in teleosts. In the current study, we investigated the antibacterial response and the participating signaling pathway initiated by R848 in golden pompano (Trachinotus ovatus). We found that R848 could stimulate the proliferation of head kidney lymphocytes (HKLs) in a dose-dependent manner, enhance the survival rate of HKLs, and inhibit the replication of bacteria in vivo. However, these effects induced by R848 were significantly reduced when chloroquine (CQ) was used to blocked endosomal acidification. Additionally, an in vivo study showed that R848 strengthened the antibacterial immunity of fish through a TLR7/8 and Myd88-dependent signaling pathway. A cellular experiment showed that Pepinh-MYD (a Myd88 inhibitor) significantly reduced the R848-mediated proliferation and survival of HKLs. Luciferase activity analysis showed that R848 enhanced the nuclear factor kappa B (NF-κB) activity, whereas this activity was reduced when CQ and Pepinh-MYD were present. Additionally, when an NF-κB inhibitor was present, the R848-mediated pro-proliferative and pro-survival effects on HKLs were significantly diminished. An in vivo study showed that knockdown of TLR7, TLR8, and Myd88 expression in golden pompano via siRNA following injection of R848 resulted in increased bacterial dissemination and colonization in fish tissues compared to that of fish injection of R848 alone, suggesting that R848-induced antibacterial immunity was significantly reduced. In conclusion, these results indicate that R848 plays an essential role in the antibacterial immunity of golden pompano via the TLR7/8-Myd88-NF-κB- signaling pathway.

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Section: Discussionmentioning

confidence: 99%

R848 Is Involved in the Antibacterial Immune Response of Golden Pompano (Trachinotus ovatus) Through TLR7/8-MyD88-NF-κB-Signaling Pathway

et al. 2021

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“…Among all known PRRs families, TLRs have been the most intensely studied group in a variety of species (Basu, Swain, Sahoo, Maiti, & Samanta, ; Chi et al., ; De Nardo, ; Gasiorowski et al, ; Li, Yang, Ma, Li, Yang et al. ; Samanta et al., ; Silk, Zhao, Weng, & Ma, ; Wei et al., ) although NLRs are the subject of a growing number of studies. Due to their diversified roles in recognizing pathogens and regulating the subsequent immune response, the NLR family of intracellular sensors has emerged as an important component of the innate immune response.…”

Section: Introductionmentioning

confidence: 99%

Nucleotide‐binding and oligomerization domain (NOD)‐like receptors in teleost fish: Current knowledge and future perspectives

Zhang

Gao

et al. 2018

Journal of Fish Diseases

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Nucleotide-binding and oligomerization domain (NOD)-like receptors (NLRs) are a group of intracellular pathogen recognition receptors (PRRs) that play key roles in pathogen recognition and subsequent activation of innate immune signalling pathways. Expressions of several NLR subfamily members, including NOD1, NOD2, NLR-C3, NLR-C5 and NLR-X1 have been reported in many different teleost fish species. These receptors are activated by a variety of ligands, including lipopolysaccharides (LPS), peptidoglycans (PGN) and polyinosinic-polycytidylic acid [Poly(I:C)]. Synthetic dsRNA and bacterial or viral infections are known to stimulate these receptors both in vitro and in vivo. In this review, we focus on the identification, expression and function of teleost NLRs in response to bacterial or viral pathogens. Additionally, NLR ligand specificity and signalling pathways involved in the recognition of bacterial or viral stimuli are also summarized. This review focuses on current knowledge in this area and provides future perspectives regarding topics in need of additional investigation. Understanding the response of innate immune system to bacterial or viral infections in diverse species could inform the development of more effective therapies and vaccines.

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“…While TLR2 homodimers have been demonstrated to recognise the conventional ligands (lipoteichoic acid and peptidoglycan) in C. carpio, its heterodimer with TLR1 was seen to be upregulated in response to the conventional TLR3 and 4 ligands-polyI:C and lipopolysaccharide, respectively 39,40 . PolyI:C challenge in T. ovatus also showed upregulation of TLR7 and 8, however TLR7 and 8a1 in O. mykiss did not respond to either to polyI:C or R848 41,42 . This is justified at molecular level by our results indicating the diversification of teleost TLRs.…”

Section: Comparative Domain Analysismentioning

confidence: 89%

Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus

Priyam

Gupta

Sarkar

et al. 2020

Sci Rep

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The high degree of conservation of toll-like receptors (TLRs), and yet their subtle variations for better adaptation of species in the host–pathogen arms race make them worthy candidates for understanding evolution. We have attempted to track the trend of TLR evolution in the most diverse vertebrate group—teleosts, where Clarias batrachus was given emphasis, considering its traits for terrestrial adaptation. Eleven C. batrachus TLRs (TLR1, 2, 3, 5, 7, 8 9, 13, 22, 25, 26) were identified in this study which clustered in proximity to its Siluriformes relative orthologues in the phylogenetic analysis of 228 TLRs from 25 teleosts. Ten TLRs (TLR1, 2, 3, 5, 7, 8 9, 13, 21, 22) with at least 15 member orthologues for each alignment were processed for selection pressure and coevolutionary analysis. TLR1, 7, 8 and 9 were found to be under positive selection in the alignment-wide test. TLR1 also showed maximum episodic diversification in its clades while the teleost group Eupercaria showed the maximum divergence in their TLR repertoire. Episodic diversification was evident in C. batrachus TLR1 and 7 alignments. These results present a strong evidence of a divergent TLR repertoire in teleosts which may be contributing towards species-specific variation in TLR functions.

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Molecular cloning and expression analysis of toll-like receptor genes (TLR7, TLR8 and TLR9) of golden pompano (Trachinotus ovatus)

Cited by 31 publications

References 39 publications

R848 Is Involved in the Antibacterial Immune Response of Golden Pompano (Trachinotus ovatus) Through TLR7/8-MyD88-NF-κB-Signaling Pathway

R848 Is Involved in the Antibacterial Immune Response of Golden Pompano (Trachinotus ovatus) Through TLR7/8-MyD88-NF-κB-Signaling Pathway

Nucleotide‐binding and oligomerization domain (NOD)‐like receptors in teleost fish: Current knowledge and future perspectives

Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus

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