Low intensity pulsed ultrasound (LIPUS) for bone healing: a clinical practice guideline

Poolman, Rudolf W.; Agoritsas, Thomas; Siemieniuk, Reed A C; Harris, Ian A.; Schipper, I.B.; Mollon, Brent; Smith, Maureen; Albin, Alexandra; Nador, S.; Sasges, W.; Schandelmaier, Stefan; Lytvyn, Lyubov; Kuijpers, Ton; Beers, Loes W A H van; Verhofstad, Michael H J; Vandvik, Per Olav

doi:10.1136/bmj.j576

Cited by 71 publications

(50 citation statements)

References 5 publications

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“…In our study, except for GCL, YAP and p-YAP were also found expressed in the INL, ONL, and OPL ( Figures 5B,C ). As LIPUS are a type of non-focus ultrasound and have been clinically used as fracture healing treatment from 1994 ( Poolman et al, 2017 ), the acoustic effect on tissue could reach to the deep layer of the retina. Therefore, the LIPUS may trigger the expression pattern of YAP and p-YAP in retina layers other than GCL, which need further elucidation.…”

Section: Discussionmentioning

confidence: 99%

Low-Intensity Pulsed Ultrasound Protects Retinal Ganglion Cell From Optic Nerve Injury Induced Apoptosis via Yes Associated Protein

Zhou

Liu

Chen

et al. 2018

Front. Cell. Neurosci.

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Background: Low-intensity pulsed ultrasound (LIPUS) has been used in clinical studies. But little is known about its effects on the central nervous system (CNS), or its mechanism of action. Retinal ganglion cells (RGCs) are CNS neuronal cells that can be utilized as a classic model system to evaluate outcomes of LIPUS protection from external trauma-induced retinal injury. In this study, we aim to: (1) determine the pulse energy and the capability of LIPUS in RGC viability, (2) ascertain the protective role of LIPUS in optic nerve (ON) crush-induced retinal injury, and 3) explore the cellular mechanisms of RGC apoptosis prevention by LIPUS.Methods: An ON crush model was set up to induce RGC death. LIPUS was used to treat mice eyes daily, and the retina samples were dissected for immunostaining and Western blot. The expression of yes-associated protein (YAP) and apoptosis-related proteins was detected by immunostaining and Western blot in vitro and in vivo. Apoptosis of RGCs was evaluated by TUNEL staining, the survival of RGCs and retained axons were labeled by Fluoro-gold and Tuj1 antibody, respectively. Rotenone was used to set up an in vitro cellular degenerative model and siYAP was used to interfering the expression of YAP to detect the LIPUS protective function.Results: LIPUS protected RGC from loss and apoptosis in vivo and in vitro. The ratio of cleaved/pro-caspase3 also decreased significantly under LIPUS treatment. As a cellular mechanical sensor, YAP expression increased and YAP translocated to nucleus in LIPUS stimulation group, however, phospho-YAP was found to be decreased. When YAP was inhibited, the LIPUS could not protect RGC from caspase3-dependent apoptosis.Conclusion: LIPUS prevented RGCs from apoptosis in an ON crush model and in vitro cellular degenerative model, which indicates a potential treatment for further traumatic ON injury. The mechanism of protection is dependent on YAP activation and correlated with caspase-3 signaling.

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Section: Discussionmentioning

confidence: 99%

Low-Intensity Pulsed Ultrasound Protects Retinal Ganglion Cell From Optic Nerve Injury Induced Apoptosis via Yes Associated Protein

Zhou

Liu

Chen

et al. 2018

Front. Cell. Neurosci.

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“…Цей метод здебільшого застосовується при лікуванні переломів трубчастих кісток (зокрема у курців), а також показаний пацієнтам, яким було проведено остеотомію [5][6][7]. У клінічних рекомендаціях Poolman RW et al, 2017 [8] визначають застосування LIPUS-терапії як найбільш безпечного методу лікування неконсолідованих переломів. Втім, автори рекомендацій зазначають, що процес лікування вимагає значних часових затрат (згідно протоколу, курс складається із щоденних 20 хвилинних сеансів упродовж 5 місяців).…”

Section: огляд та обговорення низько-інтенсивні пульсуючі ультразвукоunclassified

Modern Physical Methods of Stimulating the Bone Tissue Healing Processes

Vares¹,

Shtybel²

2019

Ukr. ž. med. bìol. sportu

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“…[ 2 ] Moreover, it is suggested that >33% of people will experience a fracture in their life time. [ 3 ] Meanwhile, 5% to 10% of these fractures show delayed healing or nonunion. [ 4 ] The delay unions or nonunions often require further intervention and may cause serious complications, such as pain and functional limitations.…”

Section: Introductionmentioning

confidence: 99%