Isochoric and isobaric freezing of fish muscle

Năstase, Gabriel; Lyu, Chenang; Ukpai, Gideon; Şerban, Alexandru; Rubinsky, Boris

doi:10.1016/j.bbrc.2017.02.091

Cited by 36 publications

(18 citation statements)

References 12 publications

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“…For example, increased lipid packing was monitored for fibroblasts frozen in the absence of a cryoprotectant, whereas 10% glycerol or 10% DMSO preserved the cell membrane structure and post-thaw viability [ 59 ]. Interestingly, cell dehydration has been shown not to occur during isochoric (constant volume) freezing, although this novel technology remains untested in cell immunotherapy [ 60 ].…”

Section: Freeze and Thaw Of T Cell Drug Productmentioning

confidence: 99%

Formulation Considerations for Autologous T Cell Drug Products

et al. 2021

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Genetically modified autologous T cells have become an established immunotherapy in the fight against cancer. The manufacture of chimeric antigen receptor (CAR) and αβ-T cell receptor (TCR) transduced T cells poses unique challenges, including the formulation, cryopreservation and fill–finish steps, which are the focus of this review. With an increasing number of marketing approvals for CAR-T cell therapies, comparison of their formulation design and presentation for administration can be made. These differences will be discussed alongside the emergence of automated formulation and fill-finish processes, the formulation design space, Monte Carlo simulation applied to risk analysis, primary container selection, freezing profiles and thaw and the use of dimethyl sulfoxide and alternative solvents/excipients as cryopreservation agents. The review will conclude with a discussion of the pharmaceutical solutions required to meet the simplification of manufacture and flexibility in dosage form for clinical treatment.

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Section: Freeze and Thaw Of T Cell Drug Productmentioning

confidence: 99%

Formulation Considerations for Autologous T Cell Drug Products

et al. 2021

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“…The survival of several types of animal and plant tissues during isochoric frozen storage at high sub‐zero temperatures have been previously investigated. For example, rat hearts after being stored at −4°C in an isochoric system were physiologically functional afterward (Wan et al, 2018), and tilapia muscle frozen at −5°C appeared identical to fresh tissue (Năstase, Lyu, Ukpai, Şerban, & Rubinsky, 2017). Additionally, Caenorhabditis elegans has been previously shown to be capable of surviving isochoric freezing at −6°C while conventional (isobaric) freezing at the same temperature was lethal (Mikus et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Viability of Listeria monocytogenes and Salmonella Typhimurium after isochoric freezing

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Bilbao‐Sainz

Powell‐Palm

et al. 2020

Journal of Food Safety

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Isochoric freezing, different from isobaric (conventional) freezing, allows for storage below freezing temperatures without significant damage from ice formation. While several types of tissues have been successfully stored in sub-zero isochoric conditions, it is unknown how isochoric freezing affects pathogenic microorganisms. Thus, the objective of this study was to investigate the survival of Salmonella Typhimurium and Listeria monocytogenes at below freezing storage (<0 C) in isochoric conditions. Tested conditions included storage at −4, −7, and −15 C for 24 hr and at −15 C for 1, 2, 3, 6, 12, and 24 hr. A comparison of bacterial survival during isobaric freezing was included with every trial. Additionally, bacterial cells were examined for morphological damage using transmission electron and field-emission scanning electron microscopes. Isochoric freezing at −15 C for 24 hr reduced both species of bacteria down to unrecoverable levels and maximum efficacy achieved after the 6 hr timepoint for L. monocytogenes and the 12 hr timepoint for S. Typhimurium. When viewed using electron microscopy, S. Typhimurium cells were noticeably disfigured with regions of cytosol separated from the cell wall. The results of this study demonstrate that isochoric freezing is capable of substantial levels of pathogen reduction. Unlike conventional nonthermal interventions, isochoric freezing does not require additional devices such as elevated pressure machines or pulsed electric fields and can be achieved with simple, inexpensive, rigid closed volume containers such as household freezers or commercial cold storage facilities.

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“…Elegans in an isochoric system at a temperature of– 5°C and pressure of 60 MPa [ 28 ]. The use of isochoric systems for food preservation was studied in another recent set of publications [ 29 – 31 ]. Last, a recent publication by Perez et al has shown that the thermodynamic properties of a two phase undissolved gaseous air–water mixture isochoric system, are substantially different from those of a single phase liquid isochoric aqueous system [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Pressure in isochoric systems containing aqueous solutions at subzero Centigrade temperatures

et al. 2017

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ObjectivePreservation of biological materials at subzero Centigrade temperatures, cryopreservation, is important for the field of tissue engineering and organ transplantation. Our group is studying the use of isochoric (constant volume) systems of aqueous solution for cryopreservation. Previous studies measured the pressure-temperature relations in aqueous isochoric systems in the temperature range from 0°C to – 20°C. The goal of this study is to expand the pressure-temperature measurement beyond the range reported in previous publications.Materials and methodsTo expand the pressure-temperature measurements beyond the previous range, we have developed a new isochoric device capable of withstanding liquid nitrogen temperatures and pressures of up to 413 MPa. The device is instrumented with a pressure transducer than can monitor and record the pressures in the isochoric chamber in real time. Measurements were made in a temperature range from – 5°C to liquid nitrogen temperatures for various solutions of pure water and Me2SO (a chemical additive used for protection of biological materials in a frozen state and for vitrification (glass formation) of biological matter). Undissolved gaseous are is carefully removed from the system.ResultsTemperature-pressure data from – 5°C to liquid nitrogen temperature for pure water and other solutions are presented in this study. Following are examples of some, temperature-pressure values, that were measured in an isochoric system containing pure water: (- 20°C, 187 MPa); (-25°C, 216 MPa); (- 30°C, 242.3 MPa); (-180°C, 124 MPa). The data is consistent with the literature, which reports that the pressure and temperature at the triple point, between ice I, ice III and water is, - 21.993°C and 209.9 MPa, respectively. It was surprising to find that the pressure in the isochoric system increases at temperatures below the triple point and remains high to liquid nitrogen temperatures. Measurements of pressure-temperature relations in solutions of pure water and Me2SO in different concentrations show that, for concentrations in which vitrification is predicted, no increase in pressure was measured during rapid cooling to liquid nitrogen temperatures. However, ice formation either during cooling or warming to and from liquid nitrogen temperatures produced an increase in pressure.ConclusionsThe data obtained in this study can be used to aid in the design of isochoric cryopreservation protocols. The results suggest that the pressure measurement is important in the design of “constant volume” systems and can provide a simple means to gain information on the occurrence of vitrification and devitrification during cryopreservation processes of aqueous solutions in an isochoric system.

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Isochoric and isobaric freezing of fish muscle

Cited by 36 publications

References 12 publications

Formulation Considerations for Autologous T Cell Drug Products

Formulation Considerations for Autologous T Cell Drug Products

Viability of Listeria monocytogenes and Salmonella Typhimurium after isochoric freezing

Pressure in isochoric systems containing aqueous solutions at subzero Centigrade temperatures

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