Biomedical Applications of Enzymes From Marine Actinobacteria

Kannan, K.; Pitchiah, Sivaperumal

doi:10.1016/bs.afnr.2016.11.002

Cited by 6 publications

(4 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the minimum inhibitory concentration of protease enzyme that prevented the growth of the bacterial strains was very close to that of the standard tetracycline (Table 3). In addition to that, our researchers are done the research area of biological activities from the different sources [38, [43][44][45][46][47]7,42,[48][49][50][51][52]3,4,[53][54][55][56][57][58][59][60].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Production of Protease Enzyme and Antimicrobial Activities from Marine Actinobacterium of Streptomyces SP

Ananya

Sivaperumal

Anitha

et al. 2021

JPRI

Self Cite

View full text Add to dashboard Cite

Introduction: Actinobacteria are Gram-positive bacteria that have traits of both bacteria and fungus. They are found in both terrestrial and aquatic habitats and aid in the breakdown of deceased species' organic matter. Marine actinobacteria are unusual in that they have antibacterial, anticancer, antiviral, insecticidal, and enzyme inhibitory activities. The biggest genus of Actinobacteria is Streptomyces. They create more than two-thirds of all-natural antibiotics that are therapeutically relevant (e.g. Neomycin, Cypemycin, Grisemycin, Bottromycins, and Chloramphenicol). Alkaline proteases are generated by a variety of microorganisms, including yeast, bacteria, and plants. The process of destroying or suppressing disease-causing bacteria is referred to as antimicrobial activity. This is accomplished using a variety of antimicrobial potential as well as antiviral properties. Aim: To find out the antimicrobial activity of protease enzymes produced by the Streptomyces sp of marine actinobacterium. Materials and Methods: A sediment sample was collected and marine Actinobacteria were isolated in Kuster’s Agar Medium (KUA) and were identified based on their chemotaxonomical characteristics. Results: Screening for protease was carried out to assess enzyme production and enzyme assay was carried out to assess the enzymatic activity. The effect and pH on enzyme production were observed. Then the antimicrobial potential of protease enzymes was tested against a few bacterial strains at optimum temperature and pH. Conclusion: From the above study, we conclude that the protease enzyme obtained from Streptomyces shows a potential antimicrobial (antibacterial) effect.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The colonies were counted and the population density has been expressed as colony-forming units per gram (CFU/g) of sediments. Morphologically distinct colonies were selected and pure cultures were obtained [42].…”

Section: Isolation Of Actinobacteriamentioning

confidence: 99%

Production of Protease Enzyme and Antimicrobial Activities from Marine Actinobacterium of Streptomyces SP

Ananya

Sivaperumal

Anitha

et al. 2021

JPRI

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to the immense potential of their secondary metabolic products, marine Actinobacteria have been regarded as an untapped treasure trove. Actinobacteria may be used to obtain medications in the future to treat important ailments such as antibiotic-resistant bacteria, cancer, a variety of viral diseases, malaria, and several infestations [ 17 , 18 ]. Actinobacteria generate a distinct class of biochemical compounds with several carbon skeletons.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of Zinc Nanoparticles From Actinobacterium Streptomyces Species and Their Biological Potential

Sivakumar,

Suresh,

Sethuraman

et al. 2024

Cureus

View full text Add to dashboard Cite

Background: In today's world, antibiotic-resistant microorganisms are a major concern. There is solid evidence that metal nanoparticles (NPs) tend to have antimicrobial properties. The most effective substitute for antibiotic resistance is the incorporation of metal NPs. The antibacterial properties of NPs are currently being explored and shown to be successful. Zinc (Zn) NPs that are biosynthesized from marine Actinobacterium proved to be more biocompatible, bioactive, and affordable. Aim: This study aims to investigate the synthesis of ZnNPs from Actinobacterium Streptomyces species and their antimicrobial effects against gram-positive and gram-negative bacteria. Materials and methods: The current study uses natural, considerably safer processes to synthesize ZnNPs from marine Actinobacteria with little to no negative side effects. It involves sample collection, identification, and isolation of Actinobacterium Streptomyces species. The isolated sample was air-dried, and extracts of ZnNPs were taken. Among the isolates from marine sediment, two Actinobacteria that generate bioactive secondary metabolites- Streptomyces species (MOSEL-ME28) and Rhodococcus rhodochrous (MOSEL-ME29)-were selected for extracellular synthesis of ZnNPs. The antimicrobial activity of the biosynthesized ZnNPs from marine Actinobacteria was analyzed against Staphylococcus (MRSA), Klebsiella pneumoniae , and Streptococcus mutans. The results were statistically analyzed and graphs were created. Results: ZnNPs obtained from Actinobacterium Streptomyces species exhibited antimicrobial effects against Staphylococcus (MRSA), Klebsiella , and Streptococcus mutans . At 280 nm wavelength, analysis of the UV spectrum showed a notable absorbance value of 1.8. The antibacterial efficacy against Staphylococcus MRSA, Klebsiella species, and Streptococcus mutans was assessed by measuring the zone of inhibition in diameter. The zones of inhibition were 8, 8, and 7 mm on the evaluation for Streptococcus mutans , S. aureus , and Klebsiella species, respectively, at a dose of 75 μg/mL. When the dosage was increased to 100 μg/mL, the inhibition zones were found to be 9.5, 9, and 7.5 mm for the respective bacterial strains. Conclusion: ZnNPs are biosynthesized from marine Actinobacterium Streptomyces species in this research study. They have a significant antimicrobial activity against both gram-positive and negative bacteria. This indicates that ZnNPs have enormous antimicrobial potential and have an extensive spectrum of applications. However, clinical trials must be completed before it can ...

show abstract

“…Further characterisation and bio active properties should be done in the further studies, and more articles in future are yet to come in various properties of extracellular polymeric substances. Streptomyces derived from extreme and understudied ecosystems such as the mangrove forests are potential sources of biologically active and therapeutically useful compounds [4,32,33].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Potential of Extra Polymeric Substances From Marine Actinobacteria Streptomyces Species

Ganta

Arivarasu

Sivaperumal

2021

JPRI

Self Cite

View full text Add to dashboard Cite

Introduction: The Actinobacteria are a phylum of Gram-positive bacteria. They can be terrestrial or aquatic. They are of great economic importance to humans because agriculture and forests depend on their contributions to soil systems. They are more abundant in soils than other media, especially in alkaline soils and soils rich in organic matter, where they constitute an important part of the microbial population. Material and Methods: The sediment sample was collected from the Gulf of Mannar, Tamilnadu. The collected sample was sun dried for 48 hrs and turned into fine powder by mortar and pestle. The colour of the mature sporulating aerial mycelium was recorded in naked eye. Results: The antioxidant potential of actinobacterial EPS was determined on the basis of their scavenging activity of the stable 1,1- diphenyl-2-picryl hydrazyl (DPPH) free radical. Different concentrations (0.5-3mg/ml) of samples were mixed with 2.9ml diphenylpicrylhydrazyl (DPPH) solution (120µM) in methanol and incubated in darkness at 37°c for 30 minutes. The absorbance was recorded at 517 nm. Data were implied as mean ± SEM. Conclusion: The produced melanin pigment from the Actinobacteria of streptomyces species was found to have potent antioxidant potential activity. Further characterisation and bio active properties should be done in the further studies, and more articles in future are yet to come in various properties of melanin pigment.

show abstract

Biomedical Applications of Enzymes From Marine Actinobacteria

Cited by 6 publications

References 86 publications

Production of Protease Enzyme and Antimicrobial Activities from Marine Actinobacterium of Streptomyces SP

Production of Protease Enzyme and Antimicrobial Activities from Marine Actinobacterium of Streptomyces SP

Biosynthesis of Zinc Nanoparticles From Actinobacterium Streptomyces Species and Their Biological Potential

Antioxidant Potential of Extra Polymeric Substances From Marine Actinobacteria Streptomyces Species

Contact Info

Product

Resources

About