Effect of a one‐step self‐etch adhesive on endogenous dentin matrix metalloproteinases

Apolônio, Fabianni Magalhães; Mazzoni, Annalisa; Angeloni, Valeria; Scaffa, Polliana Mendes Cándia; Santi, Spartaco; Sabóia, Vicente de Paulo Aragão; Tay, Franklin R.; Pashley, David H.; Breschi, Lorenzo

doi:10.1111/eos.12337

Cited by 20 publications

(12 citation statements)

References 29 publications

(69 reference statements)

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“…The tooth-cavity model method described in this study allowed us to quantitate MMP-9 in a clinicallyrelevant sample of dentin isolated from the prepared dentinal cavity walls of a single, extracted tooth. It has been confirmed that the concentrations, the distribution pattern of MMP-9 and the gelatinolytic potential of dentin matrix are variable along different dentin depths 24) . It has also been shown that the predentin and dentinal tubule regions presented higher gelatinolytic activity compared to intertubular dentin.…”

Section: Discussionmentioning

confidence: 78%

“…Previous studies of endogenous dentin MMPs had been focused on large dentin samples pooled from several extracted teeth. In these studies, the investigators were able to detect gelatinolytic/collagenolytic enzyme activities of endogenous dentin MMPs from large dentin powder samples (between 500 mg and 1 gram) pooled from several extracted teeth and internally quenched fluorescent collagen/gelatin substrate 16,18,[22][23][24] .…”

Section: Discussionmentioning

confidence: 99%

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Assaying endogenous matrix metalloproteinases (MMPs) in acid-etched dentinal cavity walls

et al. 2019

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Endogenous dentinal matrix metalloproteinases (MMPs) have been implicated in the auto-degradation of collagen fibrils within resin infiltrated layers of dentinal attachment. In order to target these proteinases, we must know which MMPs are produced and activated at the resin/dentin interface. In this study, we have optimized an extraction procedure and quantitated levels of endogenous MMPs in samples of dentin removed from the cavity walls of a single, extracted tooth. In our tooth-cavity model, an occlusal cavity (2×4×2 mm) was prepared and removed from the tooth crown, leaving surrounding dentinal walls of 1-mm-thick. The samples were pulverized with an analytic mill. Using enzyme-linked immunosorbent assay (ELISA), an average of 34.7 picograms of MMP-9 was detected in less than 300 mg of dentinal powder. This is the first study of its kind to quantitate endogenous levels of MMP in dentinal protein isolated from the cavity walls of a single, extracted tooth.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Assaying endogenous matrix metalloproteinases (MMPs) in acid-etched dentinal cavity walls

et al. 2019

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“…The neutral zinc and calcium dependent matrix metalloproteinases (MMPs) enzymes adjust the physiological and pathological metabolism of collagen-based tissues (Chaussain-Miller, Fioretti, Goldberg, & Menashi, 2006;Visse & Nagase, 2003). When dentin adhesive is applied regardless of self-etch or etch & rinse modes, MMPs can become active due to the calcium ions or acidic components (Apolonio et al, 2017;Mazzoni et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Does Q‐MIX pretreatment improve the dentin bond durability of a two‐step self‐etch adhesive?

Tuncer

Tekçe

Demırcı

et al. 2020

Microscopy Res & Technique

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This study aims to analyze and assess the effects of three particular dentin pretreatment solutions on the bond durability of a two-step self-etch adhesive (Optibond XTR) applied to dentin after ageing for 2 years. Thirty-five third molars which were extracted (n = 5 for μTBS, n = 2 for nanoleakage) were divided into five groups: Group 1:Control (no pretreatment), Group 2:17% EDTA, Group 3:2% CHX, Group 4:17% EDTA plus 2% CHX, Group 5: Q-Mix. After the pretreatments of dentin, the dentin adhesive was applied as per the guidelines provided by the manufacturer. Half of the specimens were subjected to μTBS tests for 24 hr, while the remaining half were subjected to the tests after being kept for 2 years in water storage. Also, nanoleakage was evaluated with FE-SEM by examining silver nitrate deposits. The data obtained were evaluated using a two-way analysis of variance and Tukey Post Hoc test. The dentin pretreatments did not affect the 24 hr and 2 years μTBS values for OptiBond XTR. At 24 hr, the EDTA + CHX group (50.3 ± 4.9) showed that the highest μTBS value was obtained. Water ageing significantly reduced the μTBS results and after 2 years the highest μTBS value was obtained from the Q-Mix group (37.7 ± 5.2). Different dentin pretreatments do not alter the 24-hr μTBS and were not able to preserve the bond strength after 2 years of ageing. Q-Mix was able to slow down the regression in the strength of the dentin bond as well as nanoleakage over time.

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“…These MMPs are believed to participate in practically all dental hard tissue pathologies, including dentinal caries [9,10], degradation of the resin-dentin interface of resin composite restorations [11] and dentin erosion [12]. In particular, MMP-2 and MMP-9 are important in caries progression [13,14] and degradation of the resindentin interface [10,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and immunohistochemical identification of MMP-7 in human dentin

Mazzoni

Maravić

Tezvergil‐Mutluay³

et al. 2018

Journal of Dentistry

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Objectives: Matrix metalloproteinases (MMPs) are dentinal endogenous enzymes claimed to have a vital role in dentin organic matrix breakdown. The aim of the study was to investigate presence, localization and distribution of MMP-7 in sound human dentin. Methods: Dentin was powdered, demineralized and dissolved in isoelectric focusing buffer. Resolved proteins were transferred to nitrocellulose membranes for western blotting (WB) analyses. For the zymographic analysis, aliquots of dentin protein were electrophoresed in 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis containing fluorescently labeled gelatin. Further, the concentrations of dentinal MMPs were measured using Fluorescent Microsphere Immunoassay with a human MMP-MAP multiplex kit. Pre-and post-embedding immunolabeling technique was used to investigate the localization and distribution of MMP-7 in dentin. Dentin was cryo-fractured, the fragments partially decalcified and labeled with a primary monoclonal anti-MMP-7 and a secondary antibody conjugated with gold nanoparticles. MMP-7 labelings were identified in the demineralized dentin matrix as highly electron-dense dispersed gold particles. Results: WB and zymographic analysis of extracted dentin proteins showed presence of MMP-7 (∼20-28 KDa). Further, MMP-7 was found in the supernatants of the incubated dentin beams using Fluorescent Microsphere Immunoassay. FEI-SEM and TEM analyses established MMP-7 as an intrinsic constituent of the human dentin organic matrix. Conclusion: This study demonstrated that MMP-7 is an endogenous component of the human dentin fibrillar network. Clinical significance: It is pivotal to understand the underlying processes behind dentin matrix remodeling and degradation in order to develop the most optimal clinical protocols and ensure the longevity of dental restorations.

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Effect of a one‐step self‐etch adhesive on endogenous dentin matrix metalloproteinases

Cited by 20 publications

References 29 publications

Assaying endogenous matrix metalloproteinases (MMPs) in acid-etched dentinal cavity walls

Assaying endogenous matrix metalloproteinases (MMPs) in acid-etched dentinal cavity walls

Does Q‐MIX pretreatment improve the dentin bond durability of a two‐step self‐etch adhesive?

Biochemical and immunohistochemical identification of MMP-7 in human dentin

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