Effects of high temperature on life history traits and heat shock protein expression in chlorpyrifos-resistant Laodelphax striatella

Wang, Lihua; Shan, Dan; Zhang, Yueliang; Liu, Xiangdong; Sun, Yuhan; Zhang, Zhichun; Fang, Jichao

doi:10.1016/j.pestbp.2016.08.002

Cited by 17 publications

(7 citation statements)

References 45 publications

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“…Maximum temperatures have exceeded 36°C in spring and 39°C in autumn in this region in the last 50 years (China Meteorological Data Service Center). An inducible response like the one documented here not only can protect cells against stress but also ensure that expression of the sHsps is normally very low to reduce wasteful protein synthesis, as also postulated for Hsp70 family genes (Wang et al 2017).…”

Section: Discussionmentioning

confidence: 58%

Induced expression of small heat shock proteins is associated with thermotolerance in female Laodelphax striatellus planthoppers

Wang

Zhang

Pan

et al. 2019

Cell Stress and Chaperones

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Insects are often exposed to high temperature stress in natural environments, but the mechanisms involved in thermotolerance in many insect groups like Hemiptera are not well known. To explore possible mechanisms of thermotolerance in the hemipteran pest Laodelphax striatellus, which damages rice through direct feeding and viral transmission, small heat shock proteins (sHsps) implicated in thermotolerance in other insect groups were identified. The seven sHsps identified have a conserved alpha crystallin domain, a variable N-terminal region, and shared relative low identities to each other. Three of the sHsp genes (LsHsp20.5, LsHsp21.5, and LsHsp21.6) exhibited higher basal expression than the other four genes but showed weak or no heat-induced expression. The other four genes (LsHsp20.1, LsHsp21.2, LsHsp21.4, and LsHsp22.0) were induced up to 3306-fold by heat. Injection of dsRNA indicated that expression of these sHsps was associated with thermotolerance, and Escherichia coli transformed with LsHsp21.2 and LsHsp20.1 showed relatively higher thermotolerance. These results point to an important functional role of these sHsps for thermotolerance in L. striatellus.

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Section: Discussionmentioning

confidence: 58%

Induced expression of small heat shock proteins is associated with thermotolerance in female Laodelphax striatellus planthoppers

Wang

Zhang

Pan

et al. 2019

Cell Stress and Chaperones

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“…Fecundity was lower for the resistant strain in all treatments except 18°C, and the resistant strain had a longer recovery time from heat shock. Most measured life history traits of chlorpyrifos‐resistant Laodelphax striatella under two temperature conditions, 24 and 30°C, were not different between strains (larval development time, larval survival, or adult longevity) 28 . At 24°C, the susceptible strain had higher fertility than the resistant strain, but at 30°C the strains were not different.…”

Section: Op and Carbamate Resistancementioning

confidence: 89%

Fitness studies of insecticide resistant strains: lessons learned and future directions

Freeman

Smith

Silva

et al. 2021

Pest Management Science

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The evolution of insecticide resistance is generally thought to be associated with a fitness cost in the absence of insecticide exposure. However, it is not clear how these fitness costs manifest or how universal this phenomenon is. To investigate this, we conducted a literature review of publications that studied fitness costs of insecticide resistance, selected papers that met our criteria for scientific rigor, and analyzed each class of insecticides separately as well as in aggregate. The more than 170 publications on fitness costs of insecticide resistance show that in 60% of the experiments there is a cost to having resistance, particularly for measurements of reversion of resistance and reproduction. There were differences between classes of insecticides, with fitness costs seen less commonly for organochlorines. There was considerable variation in the experiments performed. We suggest that future papers will have maximum value to the community if they quantitatively determine resistance levels, identify the resistance mechanisms present (and the associated mutations), have replicated experiments, use related strains (optimally congenic with the resistance mutation introgressed into different genetic backgrounds) and measure fitness by multiple metrics. Studies on the fitness costs of insecticide resistance will continue to enlighten our understanding of the evolutionary process and provide valuable information for resistance management. © 2021 Society of Chemical Industry

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“…The transcriptional expression profile results also indicated that the expression of AlHSP90 in female adults treated with chlorpyrifos and emamectin benzoate and in male adults treated with cyhalothrin were higher than that with other treatments [14]. Earlier studies show that one HSP70 gene was significantly upregulated in the DDT-resistant strains of Aedes aegypti [56], two HSP70s in a chlorpyrifos-resistant population of Plutella xylostella [15], one HSP70 in organophosphorus insecticide resistant Chironomus yoshimatsu [20], and two HSP70s in chlorpyrifos-resistant Laodelphax striatella [57]. One HSP70 gene has been reported to involve in cellular damage in reproductive tissues induced by cypermethrin insecticide in Drosophila melanogaster [58], and Colorado potato beetles produce more HSP70 in response of imidacloprid [59].…”

Section: Discussionmentioning

confidence: 99%

HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance

Qiao

et al. 2019

Malar J

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Background Heat shock proteins (HSPs) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress. There is still no report regarding the diversity and phylogenetics research of HSP superfamily of genes at whole genome level in insects, and the HSP gene association with pyrethroid resistance is also not well known. The present study investigated the diversity, classification, scaffold location, characteristics, and phylogenetics of the superfamily of genes in Anopheles sinensis genome, and the HSP genes associated with pyrethroid resistance. Methods The present study identified the HSP genes in the An. sinensis genome, analysed their characteristics, and deduced phylogenetic relationships of all HSPs in An. sinensis , Anopheles gambiae , Culex quinquefasciatus and Aedes aegypti by bioinformatic methods. Importantly, the present study screened the HSPs associated with pyrethroid resistance using three field pyrethroid-resistant populations with RNA-seq and RT-qPCR, and looked over the HSP gene expression pattern for the first time in An. sinensis on the time-scale post insecticide treatment with RT-qPCR. Results There are 72 HSP genes in An. sinensis genome, and they are classified into five families and 11 subfamilies based on their molecular weight, homology and phylogenetics. Both RNA-seq and qPCR analysis revealed that the expression of AsHSP90AB , AsHSP70 - 2 and AsHSP21.7 are significantly upregulated in at least one field pyrethroid-resistant population. Eleven genes are significantly upregulated in different period after pyrethroid exposure. The HSP90, sHSP and HSP70 families are proposed to be involved in pyrethroid stress response based in expression analyses of three field pyrethroid-resistant populations, and expression pattern on the time scale post insecticide treatment. The AsHSP90AB gene is proposed to be the essential HSP gene for pyrethroid stress response in An. sinensis . Conclusions This study provides the information frame for HSP superfamily of genes, and lays an important basis for the better understanding and further research of HSP function in insect adaptability to diverse environments. Electronic supplementary material The online version of this article (10.1186/s12936-019-2770-6) contains supplementary material, which is available to authorized users.

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Effects of high temperature on life history traits and heat shock protein expression in chlorpyrifos-resistant Laodelphax striatella

Cited by 17 publications

References 45 publications

Induced expression of small heat shock proteins is associated with thermotolerance in female Laodelphax striatellus planthoppers

Induced expression of small heat shock proteins is associated with thermotolerance in female Laodelphax striatellus planthoppers

Fitness studies of insecticide resistant strains: lessons learned and future directions

HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance

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