Biopsy of bovine embryos produced in vivo and in vitro does not affect pregnancy rates

Sousa, R. V.; Cardoso, Célia Regina da Silva; Butzke, Guilberth; Dode, M. A. N.; Rumpf, R.; Franco, M. M.

doi:10.1016/j.theriogenology.2016.11.003

Cited by 34 publications

(27 citation statements)

References 39 publications

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“…More importantly when considering bovine embryo production as a model for ART in human, in vitro production is accompanied by a decrease in “embryo quality” compared to in vivo development. Indeed, pregnancy rates after transfer of in vitro produced bovine embryos are 10–30% lower than for in vivo developed embryos (de Sousa et al, ; Pontes et al, ; Rasmussen et al, ). Because the ability to develop to term is difficult to test systematically due to pregnancy length in cattle (9 months) and to the fact that development to term not only depends on embryo quality but also relies on the recipient cow ability to carry out the pregnancy, embryo quality is most often assessed experimentally using experimental criteria such as embryo cryotolerance and gene expression at early stages of development.…”

Section: Effects Of Art Process On Early Embryo Developmentmentioning

confidence: 99%

Long term effects of ART: What do animals tell us?

Duranthon

Chavatte‐Palmer

2018

Molecular Reproduction Devel

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Early stages of mammalian embryonic development are now known to be very sensitive to their microenvironment, with long term effects on fetal, postnatal, and adult health, thus extending to these early stages the concept of Developmental Origin of Health and Disease (DoHaD). In this scientific context, and with 3% of births in developed countries, safety of Assisted Reproductive Techniques procedures becomes a matter of concern. Besides, embryo technologies in domestic mammals, using huge number of embryos, do not seem to evidence heavy impacts on adult phenotypes. This paper first discusses what can or cannot be concluded from farm animal data, then develops long term effects of ART procedures (ovarian stimulation, in vitro fertilization and embryo culture) evidenced in model species (mainly mouse model). Recent literature demonstrates both individual and cumulative effects of each ART procedure on fetal and postnatal phenotypes. In a second part, because they are sources for further perturbations, immediate effects of ART on early embryo phenotypes at the cellular and molecular levels are described in both farm animals and model species. Mechanistic hypotheses supporting these ART induced phenotypic alterations are subsequently considered. Finally, taking into account interspecies differences in the mechanisms likely to be involved, the relevance of results obtained in animal models for human ART are discussed.

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Section: Effects Of Art Process On Early Embryo Developmentmentioning

confidence: 99%

Long term effects of ART: What do animals tell us?

Duranthon

Chavatte‐Palmer

2018

Molecular Reproduction Devel

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“…In addition, the conjunction of biopsies obtained from non-implanted embryos or amniocentesis with GS in younger heifers has increased the genetic selection pressure even further (Kasinathan et al, 2015). Although some limitations of these approaches have been found (e.g., extra cost and ethical considerations (Kasinathan et al, 2015)), a recent study indicated that embryo biopsy does not affect the viability and pregnancy rate of IVP-derived embryo (de Sousa et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Ferré¹,

Kjelland²,

Strøbech³

et al. 2020

Animal

177

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In vitro production (IVP) of embryos and associated technologies in cattle have shown significant progress in recent years, in part driven by a better understanding of the full potential of these tools by end users. The combination of IVP with sexed semen (SS) and genomic selection (GS) is being successfully and widely used in North America, South America and Europe. The main advantages offered by these technologies include a higher number of embryos and pregnancies per unit of time, and a wider range of potential female donors from which to retrieve oocytes (including open cyclic females and ones up to 3 months pregnant), including high index genomic calves, a reduced number of sperm required to produce embryos and increased chances of obtaining the desired sex of offspring. However, there are still unresolved aspects of IVP of embryos that limit a wider implementation of the technology, including potentially reduced fertility from the use of SS, reduced oocyte quality after in vitro oocyte maturation and lower embryo cryotolerance, resulting in reduced pregnancy rates compared to in vivo–produced embryos. Nevertheless, promising research results have been reported, and work is in progress to address current deficiencies. The combination of GS, IVP and SS has proven successful in the commercial field in several countries assisting practitioners and cattle producers to improve reproductive performance, efficiency and genetic gain.

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“…When selection is based on TBV and not carrier status, more efficient editing procedures generally produce greater responses. Recent research has shown that biopsies of bovine embryos, such as might be used for genotyping, do not affect pregnancy rate [23]; therefore, success rates might be improved through more rigorous ET protocols for edited embryos even when editing technologies differ. Although the cost of producing gene-edited animals decreases as the technology becomes more efficient, this study did not examine those differences because no data on actual costs of production were publicly available.…”

Section: Resultsmentioning

confidence: 99%

Management of Mendelian Traits in Breeding Programs by Gene Editing: A Simulation Study

Cole

2017

Preprint

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Biopsy of bovine embryos produced in vivo and in vitro does not affect pregnancy rates

Cited by 34 publications

References 39 publications

Long term effects of ART: What do animals tell us?

Long term effects of ART: What do animals tell us?

Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods

Management of Mendelian Traits in Breeding Programs by Gene Editing: A Simulation Study

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