Stem cell therapy for ischemic heart diseases

Yu, Hong; Lü, Kai; Zhu, Junfeng; Wang, Jianan

doi:10.1093/bmb/ldw059

Cited by 125 publications

(108 citation statements)

References 181 publications

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“…Second, because UA-ADRCs are autologous cells, their application does not bear the risk of HLA mismatch associated with allogeneic cells (compromised clinical outcome after application of allogeneic cells was reported in [20][21][22]). Third, adipose tissue typically has a significantly higher stem cell density than bone marrow (5 to 10% vs. 0.1%), and harvesting adipose tissue can be less invasive than harvesting bone marrow [23,24]. Fourth, unlike the use of adipose derived stem cells (ASCs) which are culture-expanded from the SVF [25][26][27], the use of UA-ADRCs allows immediate usage at point of care [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Winnier¹,

Valenzuela²,

Peters-Hall³

et al. 2019

PLoS ONE

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Freshly isolated, uncultured, autologous adipose derived regenerative cells (ADRCs) have emerged as a promising tool for regenerative cell therapy. The Transpose RT system (InGeneron, Inc., Houston, TX, USA) is a system for isolating ADRCs from adipose tissue, commercially available in Europe as a CE-marked medical device and under clinical evaluation in the United States. This system makes use of the proprietary, enzymatic Matrase Reagent for isolating cells. The present study addressed the question whether the use of Matrase Reagent influences cell yield, cell viability, live cell yield, biological characteristics, physiological functions or structural properties of the ADRCs in final cell suspension. Identical samples of subcutaneous adipose tissue from 12 subjects undergoing elective lipoplasty were processed either with or without the use of Matrase Reagent. Then, characteristics of the ADRCs in the respective final cell suspensions were evaluated. Compared to non-enzymatic isolation, enzymatic isolation resulted in approximately twelve times higher mean cell yield (i.e., numbers of viable cells/ml lipoaspirate) and approximately 16 times more colony forming units. Despite these differences, cells isolated from lipoaspirate both with and without the use of Matrase Reagent were independently able to differentiate into cells of all three germ layers. This indicates that biological characteristics, physiological functions or structural properties relevant for the intended use were not altered or induced using Matrase Reagent. A comprehensive literature review demonstrated that isolation of ADRCs from lipoaspirate using the Transpose RT system and the Matrase Reagent results in the highest viable cell yield among published data regarding isolation of ADRCs from lipoaspirate.

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Section: Introductionmentioning

confidence: 99%

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Winnier¹,

Valenzuela²,

Peters-Hall³

et al. 2019

PLoS ONE

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show abstract

“…Second, because UA-ADRCs are autologous cells, their application does not bear the risk of HLA mismatch associated with allogeneic cells (compromised clinical outcome after application of allogeneic cells was reported in [20][21][22]). Third, adipose tissue typically has a significantly higher stem cell density than bone marrow (5 to 10% vs. 0.1%), and harvesting adipose tissue can be less invasive than harvesting bone marrow [23,24]. Fourth, unlike the use of adipose derived stem cells (ASCs) which are culture-expanded from the SVF (e.g., [25][26][27]), the use of UA-ADRCs allows immediate usage at point of care (e.g., [8][9][10]).…”

Section: Introductionmentioning

confidence: 99%

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Valenzuela

Alt

Winnier

et al. 2018

Preprint

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Background: Freshly isolated, unmodified autologous adipose derived regenerative cells (ADRCs) have emerged as a promising tool for regenerative cell therapy. The Transpose RT system (InGeneron, Inc., Houston, TX, USA) is a system for isolating ADRCs from adipose tissue, commercially available in Europe as a CE-marked medical device and under clinical evaluation in the United States. This system makes use of the proprietary, enzymatic Matrase Reagent for isolating cells. The present study addressed the question whether the use of Matrase Reagent influences cell yield, cell recovery, cell viability, biological characteristics, physiological functions or structural properties of the ADRCs in final cell suspension. Methods: Identical samples of subcutaneous adipose tissue from 12 subjects undergoing elective lipoplasty were processed either with or without the use of Matrase Reagent. Then, characteristics of the ADRCs in the respective final cell suspensions were evaluated. Results: Compared to non-enzymatic isolation, enzymatic isolation resulted in approximately twelve times higher mean cell yield (i.e., numbers of viable cells/ml lipoaspirate) and approximately 16 times more colony forming units (CFUs). Despite these differences, cells isolated from lipoaspirate both with and without the use of Matrase Reagent were independently able to differentiate into cells of all three germ layers. Discussion: The data of this study indicate that biological characteristics, physiological functions or structural properties relevant for the intended use were not altered or induced using Matrase Reagent. A comprehensive literature review demonstrated that isolation of ADRCs from lipoaspirate using the Transpose RT system and the Matrase Reagent results in the highest viable cell yield among all published data regarding isolation of ADRCs from lipoaspirate. ABSTRACTBackground: Freshly isolated, unmodified autologous adipose derived regenerative cells (ADRCs) have emerged as a promising tool for regenerative cell therapy. The Transpose RT system (InGeneron, Inc., Houston, TX, USA) is a system for isolating ADRCs from adipose tissue, commercially available in Europe as a CE-marked medical device and under clinical evaluation in the United States. This system makes use of the proprietary, enzymatic Matrase Reagent for isolating cells. The present study addressed the question whether the use of Matrase Reagent influences cell yield, cell recovery, cell viability, biological characteristics, physiological functions or structural properties of the ADRCs in final cell suspension. Methods: Identical samples of subcutaneous adipose tissue from 12 subjects undergoing elective lipoplasty were processed either with or without the use of Matrase Reagent. Then, characteristics of the ADRCs in the respective final cell suspensions were evaluated. Results: Compared to non-enzymatic isolation, enzymatic isolation resulted in approximately twelve times higher mean cell yield (i.e., numbers of viable cells/ml lipoaspirate) and approximately 16 tim...

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“…1). The latter includes the direct cardiac differentiation of injected SCs and the integration into the myocardium to compensate the loss of cardiomyocytes or endothelial cells [259]. However, data obtained from numerous in vitro and in vivo studies led to the conclusion that paracrine signaling is the fundamental mechanism that mediates the beneficial effects of SC therapy [260,261].…”

Section: Potential Mechanisms Of Adult Scs In Cardiovascular Regeneramentioning

confidence: 99%

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

Müller

Lemcke

Dávid

2018

Cell Physiol Biochem

169

140

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A large number of clinical trials have shown stem cell therapy to be a promising therapeutic approach for the treatment of cardiovascular diseases. Since the first transplantation into human patients, several stem cell types have been applied in this field, including bone marrow derived stem cells, cardiac progenitors as well as embryonic stem cells and their derivatives. However, results obtained from clinical studies are inconsistent and stem cell-based improvement of heart performance and cardiac remodeling was found to be quite limited. In order to optimize stem cell efficiency, it is crucial to elucidate the underlying mechanisms mediating the beneficial effects of stem cell transplantation. Based on these mechanisms, researchers have developed different improvement strategies to boost the potency of stem cell repair and to generate the “next generation” of stem cell therapeutics. Moreover, since cardiovascular diseases are complex disorders including several disease patterns and pathologic mechanisms it may be difficult to provide a uniform therapeutic intervention for all subgroups of patients. Therefore, future strategies should aim at more personalized SC therapies in which individual disease parameters influence the selection of optimal cell type, dosage and delivery approach.

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Stem cell therapy for ischemic heart diseases

Abstract: Recently, stem cell-derived exosomes emerge as a critical player in paracrine mechanism of stem cell-based therapy. It is promising to exploit exosomes-based cell-free therapy for ischemic heart diseases in the future.

Cited by 125 publications

References 181 publications

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

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