Molecular characterization of ABC transporters in marine ciliate, Euplotes crassus: Identification and response to cadmium and benzo[a]pyrene

Kim, Hokyun; Yim, Bora; Kim, Ji-Soo; Kim, Haeyeon; Lee, Young-Mi

doi:10.1016/j.marpolbul.2017.01.046

Cited by 30 publications

(9 citation statements)

References 79 publications

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“…Unfortunately, there are only a few available studies on the role of ABCC in PAHs detoxification in mollusks for establishing comparisons. In the present study, a significant induction of McoABCC was also observed, suggesting that it possibly takes part in Bap scavenging, as ABCB1 does (Costa et al, 2012;Yuan et al, 2014;Kim et al, 2017). Notably, in 100 µg/L Bap group, the mRNA expression levels of McoABCB1 and McoABCC were significantly induced at 1 dpe, sharply decreased over time, and ultimately reached a lower level compared to control group.…”

Section: Discussionsupporting

confidence: 63%

Novel ABCB1 and ABCC Transporters Are Involved in the Detoxification of Benzo(α)pyrene in Thick Shell Mussel, Mytilus coruscus

Guo

Yan

et al. 2020

Front. Mar. Sci.

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ATP-binding cassette (ABC) transporters play an essential role in xenobiotic resistance through transporting xenobiotics into or out of cells. However, their functional role in mollusk's defense against polycyclic aromatic hydrocarbons (PAHs) remains unclear. In the present study, novel McoABCB1 and McoABCC transporters were identified in the gills of Mytilus coruscus, and their benzo(α)pyrene (Bap) detoxification function was determined by qRT-PCR and efflux assays. McoABCB1 and McoABCC genes were constitutively expressed in all tissue types, suggesting the primary physiological function of these transporters. Bap, a chemical stress agent, could induce the mRNA expression of McoABCB1 and McoABCC in gills. However, at high concentration, Bap inhibited their expression levels at the late stage. Besides, the efflux activities in M. coruscus gills showed a dose-dependent elevation in a Bap pre-exposure group, compared with those in a reversin 205 or MK571 treatment alone group. These results demonstrated the presence of ABCB1 and ABCC transporters in Mytilus coruscus, and their involvement in cellular Bap detoxification.

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Section: Discussionsupporting

confidence: 63%

Novel ABCB1 and ABCC Transporters Are Involved in the Detoxification of Benzo(α)pyrene in Thick Shell Mussel, Mytilus coruscus

Guo

Yan

et al. 2020

Front. Mar. Sci.

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“…Especially, micro-RNAs(miRNAs) extensively affect their protein level through interaction with target mRNA [ 38 ]. Previous research reported B[a]P induces the mRNA expression of ABCCs, while inhibiting the protein level and function of ABCC transporters [ 39 , 40 ]. In our results, quercetin or isorhamnetin in combination with B[a]P increased both mRNA and protein levels of ABCCs compared to only the B[a]P treated group ( Figure 4 and Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Quercetin and Isorhamnetin Attenuate Benzo[a]pyrene-Induced Toxicity by Modulating Detoxification Enzymes through the AhR and NRF2 Signaling Pathways

et al. 2021

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Benzo[a]pyrene, classified as a Group 1 carcinogen, is metabolized to B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), causing DNA mutations and eventually cancer. Quercetin is a dietary flavonoid abundant in fruits and vegetables. After quercetin intake, quercetin’s metabolites isorhamnetin and miquelianin are more highly concentrated than quercetin in the human plasma. In this study, we investigated the molecular mechanisms associated with the cytoprotective effect of quercetin and its metabolites against benzo[a]pyrene from a detoxification perspective. Quercetin and its metabolite isorhamnetin reduced benzo[a]pyrene-induced cytotoxicity, whereas the metabolite miquelianin did not mitigate benzo[a]pyrene-induced cytotoxicity. Moreover, quercetin and isorhamnetin reduced intracellular levels of BPDE-DNA adducts. The formation and elimination of BPDE is mediated by the xenobiotic detoxification process. Quercetin and isorhamnetin increased the gene and protein expression levels of phase I, II, and III enzymes involved in xenobiotic detoxification. Furthermore, quercetin and isorhamnetin induced the translocation of aryl hydrocarbon receptor (AhR) and nuclear factor erythroid 2-related factor 2 (NRF2), which regulate the expression level of phase enzymes. Our results suggest that quercetin and isorhamnetin promote the metabolism, detoxification, and elimination of B[a]P, thereby increasing anti-genotoxic effects and protecting against B[a]P-induced cytotoxicity.

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“…This last antecedent suggests that the BaP can get into the cell causing toxic effects. As an example, there is Euplotes crassus, where it was observed that an ABC transporter allowed the access of BaP into the cell which then inhibited the activity of the transporter, causing the cellular toxicity of the protozoan (Kim et al, 2017). However, in the strain H23 we observed that the presence of BaP did not have any toxic effect and this result is attributed to the last ORF that encodes for a monooxygenase protein.…”

Section: Discussionmentioning

confidence: 75%

“…The second ORF codes for a membrane transport protein type major facilitator superfamily (MFS), which are proteins that facilitate movement of small solutes across cell membranes in response to chemiosmotic gradients in prokaryote cells. They participate in the detoxification of endogenous and exogenous metabolites (Dean et al, 2001;Biemans-Oldehinkel et al, 2006;Oldham et al, 2008;Kim et al, 2017). This last antecedent suggests that the BaP can get into the cell causing toxic effects.…”

Section: Discussionmentioning

confidence: 99%

Research Article Genetic expression of pobA and fabHB in Bacillus licheniformis M2-7 in the presence of benzo[a]pyrene

Rojas-Aparicio¹,

Hernández-Eligio²,

Toribio-Jiménez³

et al. 2018

Genet. Mol. Res.

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Bacillus licheniformis M2-7 is a heat-resistant bacterium able to biotransform polycyclic aromatic hydrocarbons. It can transform a wide range of these compounds as naphthalene, phenanthrene, pyrene and benzo[a]pyrene. Benzo[a]pyrene is a polycyclic aromatic hydrocarbon of high molecular weight considered as potentially toxic and carcinogenic for humans. Aiming to discover the genes involved in the biotransformation of benzo[a]pyrene, we made a B. licheniformis M2-7 genomic library in E. coli. We isolated two E. coli strains that were able to grow in minimal salt medium supplemented with benzo[a]pyrene. From the analysis of the DNA fragments in the clones H23 and H38, we identified open reading frames coding for 5 possible genes, among them pobA and fabHB, which products are the enzymes 4hydroxybenzoate 3-monooxygenase and the ketoacyl-ACP synthase Genetics and Molecular Research 17 (2): gmr16039916 III, respectively. To evaluate the role of these genes in the metabolism of benzo[a]pyrene in B. licheniformis M2-7, we estimated their relative expression through reverse transcription quantitative PCR. Finally, we observed that the genes pobA and fabHB were overexpressed after 3 h under induction with benzo[a]pyrene, suggesting that this strain could use these genes during the metabolism of this PAH, plus it does it in a faster time than that reported for other bacterial genera

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Molecular characterization of ABC transporters in marine ciliate, Euplotes crassus: Identification and response to cadmium and benzo[a]pyrene

Cited by 30 publications

References 79 publications

Novel ABCB1 and ABCC Transporters Are Involved in the Detoxification of Benzo(α)pyrene in Thick Shell Mussel, Mytilus coruscus

Novel ABCB1 and ABCC Transporters Are Involved in the Detoxification of Benzo(α)pyrene in Thick Shell Mussel, Mytilus coruscus

Quercetin and Isorhamnetin Attenuate Benzo[a]pyrene-Induced Toxicity by Modulating Detoxification Enzymes through the AhR and NRF2 Signaling Pathways

Research Article Genetic expression of pobA and fabHB in Bacillus licheniformis M2-7 in the presence of benzo[a]pyrene

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