Early changes of the pH of the apoplast are different in leaves, stem and roots of Vicia faba L. under declining water availability

Karuppanapandian, Thirupathi; Geilfus, Christoph‐Martin; Mühling, Karl H.; Novák, Ondřej; Gloser, Vít

doi:10.1016/j.plantsci.2016.11.010

Cited by 24 publications

(28 citation statements)

References 41 publications

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“…Kleaf decreased when the BSCs were brought into direct contact with ABA fed to the detached leaf, and not when ABA was smeared on the leaf surface. Our current work outlines a mechanism for this phenomenon: stress-induced xylem alkalinization (already reported by others (Jia and Davies, 2007;Wang et al, 2012;Korovetska et al, 2014;Karuppanapandian et al, 2017), likely due to ABA inhibition of the BSCs AHA2…”

Section: Aha2 Role In the Regulation Of The Whole Leaf Water Balancesupporting

confidence: 71%

“…xylem sap and alkalinization in the shoot xylem sap (Karuppanapandian et al, 2017). demonstrated that three aquaporins expressed in veins, and in particular, PIP2;1, contributed to darkness-enhanced hydraulic water conductance of the Arabidopsis leaves rosette (Prado et al, 2013).…”

Section: Extracellular Ph Regulation Of the Membrane Osmotic Water Pementioning

confidence: 93%

“…Barley leaf substomatal apoplast also alkalinized upon roots exposure to salts (KCl, NaCl, NaNO3 (Felle et al, 2005)). Drying soil in tomato (Jia and Davies, 2007) and in Vicia faba (Karuppanapandian et al, 2017) or cold stress at the root level in barley (Felle et al, 2005) also caused alkalinization (measured, respectively, in the tomato xylem sap, in the V. faba…”

Section: Introductionmentioning

confidence: 99%

“…For example, ABA accumulated in the alkalinizing xylem sap of a gradually pressure-dehydrated detached cotton leaf, which could be prevented by pretreatment with an H + -ATPase-activating fungal toxin, fusicoccin (Hartung et al, 1988). Leaf xylem alkalinization in Vicia faba as a result of gradually drying soil was followed by elevated ABA levels in the xylem (even if with a few days delay (Karuppanapandian et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H⁺-ATPase of vascular bundle sheath cells

Grunwald

Wigoda

Sade

et al. 2017

Preprint

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The leaf vascular bundle sheath cells (BSCs) that tightly envelop the leaf veins, are a selective and dynamic barrier to xylem-sap water and solutes radially entering the mesophyll cells. Under normal conditions, xylem-sap pH of <6 is presumably important for driving and regulating the transmembranal solute transport. Having discovered recently a differentially high expression of a BSC proton pump, AHA2, we now test the hypothesis that it regulates this pH and leaf radial water fluxes.We monitored the xylem-sap pH using the ratiometric fluorescent probe FITC-dextran fed into veins of detached leaves of WT Arabidopsis, AHA mutants, and aha2 mutants complemented with AHA2 gene solely in BSCs. We tested an AHA inhibitor and stimulator, and different pH buffers. We monitored their impact on the xylem-sap pH, the whole leaf hydraulic conductance (Kleaf) and the water osmotic permeability of isolated BSCs protoplasts (Pf).Our results demonstrated AHA2 indispensability for xylem-sap acidification, necessary, in turn, for elevating Pf and Kleaf. Conversely, elevating xylem-sap pH to 7.5, reduced significantly both Pf and Kleaf.All these demonstrate a causative link between AHA2 activity in BSCs and leaf water influx. This positions the BSCs as a pH-controlled transpiration valve in series with the stomata.One-sentence summaryBundle-sheath cells can control the leaf hydraulic conductance by proton-pump-regulated xylem sap pH

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Section: Aha2 Role In the Regulation Of The Whole Leaf Water Balancesupporting

confidence: 71%

Section: Extracellular Ph Regulation Of the Membrane Osmotic Water Pementioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H⁺-ATPase of vascular bundle sheath cells

Grunwald

Wigoda

Sade

et al. 2017

Preprint

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“…Abscisic acid exists naturally in plants as both an anionic form (ABA − ) and a protonated form (ABAH). ABAH can diffuse passively through the plasma membrane, and the diffusion of ABA largely declines with alkalization of the cytoplasm which increases during osmotic stresses (Wilkinson and Davies 1997; Karuppanapandian et al 2017). The active transport of ABA relies on: (i) ATP‐binding cassette (ABCG) transporters; (ii) NRT1/PTR (NPF); (iii) multidrug and toxic compound extrusion (MATE)‐type/DTX transporters (DTX50); and (iv) AWPM‐19 family proteins (OsPM1), which were originally identified in rice (Kuromori et al 2010, 2011; Kanno et al 2012; Zhang et al 2014; Kang et al 2015; Yao et al 2018).…”

Section: Metabolic Control Of Aba Levelsmentioning

confidence: 99%

Abscisic acid dynamics, signaling, and functions in plants

Chen

Bressan

et al. 2020

JIPB

926

628

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Abscisic acid (ABA) is an important phytohormone regulating plant growth, development, and stress responses. It has an essential role in multiple physiological processes of plants, such as stomatal closure, cuticular wax accumulation, leaf senescence, bud dormancy, seed germination, osmotic regulation, and growth inhibition among many others. Abscisic acid controls downstream responses to abiotic and biotic environmental changes through both transcriptional and posttranscriptional mechanisms. During the past 20 years, ABA biosynthesis and many of its signaling pathways have been well characterized. Here we review the dynamics of ABA metabolic pools and signaling that affects many of its physiological functions.

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Root pH variation of herbaceous plants among plant functional groups in response to climate and soil gradients on the Tibetan alpine grasslands

Zhao,

Pang,

Hong

et al. 2024

Ecology and Evolution

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Plant pH is an emerging functional trait that plays important roles in physiological processes and nutrient cycling. However, how root pH varies among plant functional groups (PFGs) and the regulatory factors on a large scale remain unclear. Therefore, we quantified root pH variation of herbaceous plants in four PFGs from 20 sites on the Tibetan Plateau along a 1600 km transect and explored the correlations between root pH and different PFGs, climate and soil conditions. The results showed that the root pH of herbaceous plants was slightly acidic (6.46 ± 0.05). Grasses had the highest root pH (6.91 ± 0.10) across all functional groups (p < .05), whereas legumes had the lowest (5.90 ± 0.08; p < .05). The root pH decreased with mean annual precipitation, aridity index, soil water content and soil stress coefficient, whereas the significant positive correlation with soil pH. PFGs, climate and soil explained 5.39, 11.15 and 24.94% of the root pH variance, respectively. This study provided a comprehensive analysis of root pH patterns in herbaceous plants over a large spatial scale. Root pH was controlled by the combined influence of PFGs, climate and soil properties, with moisture status being the main influential factor. In contrast to the leaf pH, the root pH of herbaceous plants is strongly affected by the soil pH along environmental gradients. Our findings provide new insights into root functional traits and survival strategies of herbaceous plants in alpine ecosystems.

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Early changes of the pH of the apoplast are different in leaves, stem and roots of Vicia faba L. under declining water availability

Cited by 24 publications

References 41 publications

Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H⁺-ATPase of vascular bundle sheath cells

Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H⁺-ATPase of vascular bundle sheath cells

Abscisic acid dynamics, signaling, and functions in plants

Root pH variation of herbaceous plants among plant functional groups in response to climate and soil gradients on the Tibetan alpine grasslands

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Early changes of the pH of the apoplast are different in leaves, stem and roots of Vicia faba L. under declining water availability

Cited by 24 publications

References 41 publications

Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H+-ATPase of vascular bundle sheath cells

Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H+-ATPase of vascular bundle sheath cells

Abscisic acid dynamics, signaling, and functions in plants

Root pH variation of herbaceous plants among plant functional groups in response to climate and soil gradients on the Tibetan alpine grasslands

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Product

Resources

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Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H⁺-ATPase of vascular bundle sheath cells

Arabidopsis leaf hydraulic conductance is regulated by xylem-sap pH, controlled, in turn, by a P-type H⁺-ATPase of vascular bundle sheath cells