Cystatin C peptidomimetic derivative with antimicrobial properties as a potential compound against wound infections

Pikuła, Michał; Smużyńska, Maria; Krzystyniak, Adam; Zieliński, Maciej; Langa, Paulina; Deptuła, Milena; Schumacher, Adriana; Łata, J; Cichorek, M; Grubb, Anders; Trzonkowski, Piotr; Kasprzykowski, Franciszek; Rodziewicz‐Motowidło, Sylwia

doi:10.1016/j.bmc.2017.01.004

Cited by 10 publications

(9 citation statements)

References 33 publications

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“…Therefore, FBS is often removed for a short period of time during the cell culture (e.g. testing drug candidates) or shortly before the clinical application of cellular product 9 , 10 . However, the effect of FBS deprivation on ACSs has not been evaluated in comprehensive way.…”

Section: Discussionmentioning

confidence: 99%

“…proliferation, differentiation) 9 . Therefore, FBS is often removed for a short period of time during cell culture (e.g., testing drug candidates) or shortly before the clinical application of cellular product 9 , 10 . Hence, it is critical to evaluate the impact of short-term serum deprivation of FBS on the adipose-derived stem cells activity 2 , 11 .…”

Section: Introductionmentioning

confidence: 99%

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Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells

Mieczkowska

Schumacher

Filipowicz

et al. 2018

Sci Rep

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Adipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell surface marker transcripts and the encoded proteins after each passage. In parallel, we investigated the differentiation potential of ASCs into chondrocytes, osteocytes and adipocytes. We further determined the effects of FBS supplementation and subsequent deprivation on the whole transcriptome by comprehensive mRNA and miRNA sequencing. Our results show that ASCs maintain differentiation potential and consistent profile of key mesenchymal markers, with apparent expression of distinct isoforms, in long-term cultures. No significant differences were observed between plastic and oncological surgery cohorts. ASCs in FBS supplemented primary cultures are almost committed to mesenchymal lineages as they express key epithelial-mesenchymal transition genes including early mesenchymal markers. Furthermore, combined mRNA/miRNA expression profiling strongly supports a modulatory role for the miR-30 family in the commitment process to mesenchymal lineages. Finally, we propose improvements to existing qPCR based assays that address alternative isoform expression of mesenchymal markers.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells

Mieczkowska

Schumacher

Filipowicz

et al. 2018

Sci Rep

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“…In the BAT protocol, whole blood basophils are challenged with the tested compound or one of the basophil activators anti-FcɛRI or fMLP, which activates basophils in an immunologic or nonimmunologic way. The expression of three markers is then checked: CCR3 (basophil population marker) and CD63 and CD203c (markers of basophil activation) [ 61 ]. In our laboratory, we have used the BAT test to evaluate the allergic potential of antimicrobial compounds (A20, camel, citropin).…”

Section: Tests Useful In Prediction Of Peptide Immunogenicity and mentioning

confidence: 99%

“…The results showed that antimicrobial peptides and peptidomimetics can have different allergic potentials. A20, also called Cystapep 1 (peptidomimetic), did not activate basophils obtained from healthy donors, but camel and citropin (peptides) had an allergic potential in hypersensivitive patients [ 54 , 61 ]. This shows that people suffering from allergies should be taken into consideration in preclinical testing of new potential peptide drugs.…”

Section: Tests Useful In Prediction Of Peptide Immunogenicity and mentioning

confidence: 99%

“…Second, challenging of activated PBMCs (e.g., by Concanavalin A, a T-cell mitogen, which stimulates release of different cytokines) with the tested peptide can show whether it decreases secretion of cytokines and exerts immunomodulatory properties. Release of TNF-α, an early marker of immune system activation, by PBMCs can also be assessed [ 61 ]. The secretion of proinflammatory cytokines (i.e., TNF-α, IL-1 etc.)…”

Section: Tests Useful In Prediction Of Peptide Immunogenicity and mentioning

confidence: 99%

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Antibacterial Peptides in Dermatology–Strategies for Evaluation of Allergic Potential

et al. 2018

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During recent decades, the market for peptide-based drugs, including antimicrobial peptides, has vastly extended and evolved. These drugs can be useful in treatment of various types of disorders, e.g., cancer, autoimmune diseases, infections, and non-healing wounds. Although peptides are less immunogenic than other biologic therapeutics, they can still induce immune responses and cause allergies. It is important to evaluate the immunogenic and allergic potential of peptides before they are forwarded to the expensive stages of clinical trials. The process of the evaluation of immunogenicity and cytotoxicity is complicated, as in vitro models and bioinformatics tools cannot fully simulate situations in the clinic. Nevertheless, several potentially promising tests for the preclinical evaluation of peptide drugs have been implemented (e.g., cytotoxicity assays, the basophil activation test, and lymphocyte activation assays). In this review, we focus on strategies for evaluation of the allergic potential of peptide-based therapeutics.

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Rapid Conductometric Sensing of Chronic Kidney Disease Biomarkers: Specific and Precise Detection of Creatinine and Cystatin C in Artificial Saliva

Yang,

Perera,

Dhanabalan

et al. 2024

Advanced Sensor Research

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Chronic kidney disease (CKD) has asymptomatic early stages, whereby early detection is crucial to prevent its complications and progression. Creatinine and cystatin C (cysC) assays are known for assessing kidney function but there are limited point‐of‐care diagnostics which are rapid, precise, and easy to use. Here, high resistivity silicon conductometric sensors for detection of creatinine and cysC with a 10 min sample incubation is introduced. The sensors provide resistance‐based signals that can be quantified and measured wirelessly. The sensors successfully detect creatinine and cysC in both phosphate buffer saline (PBS) and artificial saliva in the nanomolar range, being able to distinguish their critical concentrations at 8.8 and 20 nm, respectively, for diagnosis of early stage of CKD. The detection limit for both creatinine and cysC is determined as 0.01 nm which is more than 500× and 1000× times lower than critical concentrations for the two biomarkers, respectively. Finally, these sensors are incorporated into a battery‐free, miniaturized electronic device for wireless biomarker detection as a proof‐of‐concept demonstration of a point‐of‐care tool for assessing kidney functionality.

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Cystatin C peptidomimetic derivative with antimicrobial properties as a potential compound against wound infections

Cited by 10 publications

References 33 publications

Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells

Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells

Antibacterial Peptides in Dermatology–Strategies for Evaluation of Allergic Potential

Rapid Conductometric Sensing of Chronic Kidney Disease Biomarkers: Specific and Precise Detection of Creatinine and Cystatin C in Artificial Saliva

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