Smoking status regulates a novel panel of PIWI-interacting RNAs in head and neck squamous cell carcinoma

Krishnan, Aswini R.; Korrapati, Avinaash; Zou, Angela E.; Qu, Yuanhao; Wang, Xiao Qi; Califano, Joseph A.; Wang‐Rodriguez, Jessica; Lippman, Scott M.; Hovell, Melbourne F.; Ongkeko, Weg M.

doi:10.1016/j.oraloncology.2016.12.022

Cited by 25 publications

(20 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, we found a variety of piRNAs to be expressed in KC from healthy skin. Of 190 piRNAs identified in KC in this study, 33 have previously been described to be expressed in squamous epithelial cells [24] supporting the specificity of our findings. Presently, the significance of piRNA originating outside of transposable elements is largely unknown and piRNAs have already been shown to play a crucial role in host gene regulation [38].…”

Section: Discussionsupporting

confidence: 90%

PIWIL-2 and piRNAs are regularly expressed in epithelia of the skin and their expression is related to differentiation

et al. 2020

View full text Add to dashboard Cite

PIWI proteins play multiple roles in germline stem cell maintenance and self-renewal. PIWI-interacting RNAs (piRNAs) associate with PIWI proteins, form effector complexes and maintain genome integrity and function in the regulation of gene expression by epigenetic modifications. Both are involved in cancer development. In this study, we investigated the expression of PIWIL-2 and piRNAs in normal human skin and epithelial tumors and its regulation during keratinocyte (KC) differentiation. Immunohistochemistry showed that PIWIL-2 was regularly expressed in the epidermis and adnexal tissue with strongest expression in sebaceous glands. Cell culture studies revealed an association of PIWIL-2 expression with the state of differentiated KC. In contrast, the PIWIL-2 expression pattern did not correlate with stem cell compartments or malignancy. piRNAs were consistently detected in KC in vitro by next-generation sequencing and the expression levels of numerous piRNAs were regulated during KC differentiation. Epidermal piRNAs were predominantly derived from processed snoRNAs (C/D-box snoRNAs), tRNAs and protein coding genes. Our data indicate that components of the PIWIL-2-piRNA pathway are present in epithelial cells of the skin and are regulated in the context of KC differentiation, suggesting a role of somatic gene regulation. However, putative roles in the maintenance of stem cell compartments or the development of malignancy in the skin were not supported by this study.

show abstract

Section: Discussionsupporting

confidence: 90%

PIWIL-2 and piRNAs are regularly expressed in epithelia of the skin and their expression is related to differentiation

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Intriguingly the PIWI-piRNA system has been suggested to regulate essential cellular functions of relevance to the repair of somatic tissues, including cell proliferation, viability, cell invasion and migration [22]. However, most of the newly gained knowledge of the functional role of piRNA is based on cancer studies, including studies on skin cancers [23,24] and oral cancers [25][26][27][28]. It is tempting to hypothesize that piRNAs play a role in wound healing.…”

Section: Discussionmentioning

confidence: 99%

Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing

Lin

Chen

Simões

et al. 2020

IJMS

View full text Add to dashboard Cite

The oral mucosa exhibits exceptional healing capability when compared to skin. Recent studies suggest that intrinsic differences in coding genes and regulatory small non-coding RNA (sncRNA) genes (e.g., microRNAs) may underlie the exceptional healing that occurs in the oral mucosa. Here, we investigate the role of a novel class of sncRNA—Piwi-interacting RNA (piRNA)—in the tissue-specific differential response to injury. An abundance of piRNAs was detected in both skin and oral mucosal epithelium during wound healing. The expression of PIWI genes (the obligate binding partners of piRNAs) was also detected in skin and oral wound healing. This data suggested that PIWI-piRNA machinery may serve an unknown function in the highly orchestrated wound healing process. Furthermore, unique tissue-specific piRNA profiles were obtained in the skin and oral mucosal epithelium, and substantially more changes in piRNA expression were observed during skin wound healing than oral mucosal wound healing. Thus, we present the first clue suggesting a role of piRNA in wound healing, and provide the first site-specific piRNA profile of skin and oral mucosal wound healing. These results serve as a foundation for the future investigation of the functional contribution(s) of piRNA in wound repair and tissue regeneration.

show abstract

“…It was also reported that a collection of 13 piRNAs was found in HNSCC related to smoking. Among those 13 piRNAs, 2 piRNAs are shown to be linked with tumor stage while one piRNA (NON-HSAT067200) is shown to be a potential indicator of patient survival rate [70].…”

Section: Pirnas In Cancermentioning

confidence: 99%

The disease-related biological functions of PIWI-interacting RNAs (piRNAs) and underlying molecular mechanisms

Han

2019

ExRNA

View full text Add to dashboard Cite

More than a decade ago, PIWI-interacting RNA (piRNA) was discovered almost simultaneously by four different research groups. The length of this type of single stranded noncoding RNA is 24~31 nucleotides (nt), with most of the piRNAs fall into the range of 29~30 nt. PiRNAs form specific RNA-induced silencing complex with PIWI subfamily proteins, which is how piRNA got its name. PiRNAs are initially famous for the crucial roles they played in germline cells. Binding with PIWI family proteins, piRNAs is able to affect methylation of genomic DNA in germline cells and, therefore, maintaining genomic stability and suppressing transposons. Since mammalian PIWI subfamily proteins are mainly germline specific, it was once thought that piRNAs might only function in the gonadal cells. However, evidence from the later research suggest that piRNAs are broadly expressed in many kinds of somatic cells and are involved in numerous pathological condition far beyond those have been reported in the germline. For example, piRNAs were discovered to be abnormally expressed in several kinds of cancers. PiRNAs are also shown to be promising prognostic markers for various types of cancers. Interestingly, a recent research indicated that piRNAs are also regulators for pancreatic beta cell function. PiRNAs are promising regulators for the development of type 2 diabetes. From a disease-oriented point of view, this review will focus on both confirmed and proposed biological functions of piRNAs mostly in those fields beyond germline cells. Meanwhile, some of the underlying molecular mechanisms will also be mentioned.

show abstract

Smoking status regulates a novel panel of PIWI-interacting RNAs in head and neck squamous cell carcinoma

Cited by 25 publications

References 39 publications

PIWIL-2 and piRNAs are regularly expressed in epithelia of the skin and their expression is related to differentiation

PIWIL-2 and piRNAs are regularly expressed in epithelia of the skin and their expression is related to differentiation

Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing

The disease-related biological functions of PIWI-interacting RNAs (piRNAs) and underlying molecular mechanisms

Contact Info

Product

Resources

About